Amperometric measurements of catecholamine release from single vesicles in MN9D cells

Dong, Yan; Heien, Michael L.; Maxson, Marc M.; Ewing, Andrew G.

doi:10.1111/j.1471-4159.2008.05721.x

Cited by 28 publications

(21 citation statements)

References 28 publications

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“…First we established that our model, MN9Dsyn cells, express the rate-limiting enzyme for catecholamine synthesis, tyrosine hydroxylase, dopamine transporter and vesicular monoamine transporter 2, which is consistent with previous studies that demonstrated the ability of the MN9D parental cells to produce, transport and store dopamine (Choi et al, 1991a; Chen et al, 2005; Zhou et al, 2006; Dong et al, 2008). We also demonstrated an enhancement of α-synuclein-induced toxicity in the presence of both dopamine and paraquat.…”

Section: Discussionsupporting

confidence: 89%

Dopamine and Paraquat Enhance α-Synuclein-Induced Alterations in Membrane Conductance

Feng

Maguire‐Zeiss

2011

Neurotox Res

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We have previously demonstrated that α-synuclein overexpression increases the membrane conductance of dopaminergic-like cells. Although α-synuclein is thought to play a central role in the pathogenesis of several neurodegenerative diseases including Parkinson’s disease, multiple system atrophy and diffuse Lewy body disease the mechanism of action is not completely understood. In this study we sought to determine whether multiple factors act together with α-synuclein to engender cell vulnerability through an augmentation of membrane conductance. Here we employed a cell model that mimics dopaminergic neurons coupled with α-synuclein overexpression and oxidative stressors. We demonstrate an enhancement of α-synuclein-induced toxicity in the presence of combined treatment with dopamine and paraquat, two molecules known to incite oxidative stress. In addition we show that combined dopamine and paraquat treatment increases the expression of heme oxygenase-1, an antioxidant response protein. Finally, we demonstrate for the first time that combined treatment of dopaminergic cells with paraquat and dopamine enhances α-synuclein-induced leak channel properties resulting in increased membrane conductance. Importantly, these increases are most robust when both paraquat and dopamine are present suggesting the need for multiple oxidative insults to augment α-synuclein-induced disruption of membrane integrity.

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Section: Discussionsupporting

confidence: 89%

Dopamine and Paraquat Enhance α-Synuclein-Induced Alterations in Membrane Conductance

Feng

Maguire‐Zeiss

2011

Neurotox Res

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“…With current sampling rates in the kHz range, this technique can resolve signals on time scales below milliseconds. This technique has been successfully used for studies of catecholamine concentrations in the brain and in brain slices [125, 126], exocytosis of the small synaptic vesicles [127], neuroblastoma, and other cells [128, 129]; it has the best temporal resolution because of sampling rates down to 1 ms. However, the disadvantage of DC amperometry is that it is essentially nonselective, because all electroactive compounds that oxidize (or reduce) at the applied potential will produce a faradaic response at the electrode.…”

Section: Electrochemical Techniques For Detection Of Dopamine With Immentioning

confidence: 99%

New trends in the electrochemical sensing of dopamine

2012

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Since the early 70s electrochemistry has been used as a powerful analytical technique for monitoring electroactive species in living organisms. In particular, after extremely rapid evolution of new micro and nanotechnology it has been established as an invaluable technique ranging from experiments in vivo to measurement of exocytosis during communication between cells under in vitro conditions. This review highlights recent advances in the development of electrochemical sensors for selective sensing of one of the most important neurotransmitters—dopamine. Dopamine is an electroactive catecholamine neurotransmitter, abundant in the mammalian central nervous system, affecting both cognitive and behavioral functions of living organisms. We have not attempted to cover a large time-span nor to be comprehensive in presenting the vast literature devoted to electrochemical dopamine sensing. Instead, we have focused on the last five years, describing recent progress as well as showing some problems and directions for future development.

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“…2–6 For instance, several reports using amperometry have found significant quantitative and kinetic information, and have proposed various cellular or molecular mechanisms controlling exocytosis. 7–11 …”

Section: Introductionmentioning

confidence: 99%

Carbon-Ring Microelectrode Arrays for Electrochemical Imaging of Single Cell Exocytosis: Fabrication and Characterization

et al. 2012

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Fabrication of carbon microelectrode arrays, with up to 15 electrodes in total tips as small as 10 to 50 μm, is presented. The support structures of microelectrodes were obtained by pulling multiple quartz capillaries together to form hollow capillary arrays before carbon deposition. Carbon ring microelectrodes were deposited by pyrolysis of acetylene in the lumen of these quartz capillary arrays. Each carbon deposited array tip was filled with epoxy, followed by beveling of the tip of the array to form a deposited carbon-ring microelectrode array (CRMA). Both the number of the microelectrodes in the array and the tip size are independently tunable. These CRMAs have been characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, and electrogenerated chemiluminescence. Additionally, the electrochemical properties were investigated with steady-state voltammetry. In order to demonstrate the utility of these fabricated microelectrodes in neurochemistry, CRMAs containing eight microring electrodes were used for electrochemical monitoring of exocytotic events from single PC12 cells. Subcellular temporal heterogeneities in exocytosis (ie. cold spots vs. hot spots) were successfully detected with the CRMAs.

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Amperometric measurements of catecholamine release from single vesicles in MN9D cells

Cited by 28 publications

References 28 publications

Dopamine and Paraquat Enhance α-Synuclein-Induced Alterations in Membrane Conductance

Dopamine and Paraquat Enhance α-Synuclein-Induced Alterations in Membrane Conductance

New trends in the electrochemical sensing of dopamine

Carbon-Ring Microelectrode Arrays for Electrochemical Imaging of Single Cell Exocytosis: Fabrication and Characterization

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