AMPK CA(R)Sts a new light on amino acid sensing

Brown, Kristin K.

doi:10.15252/embj.2021109575

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Cellular bioenergetics deficiency is a critical trigger for autophagy induction [45][46] . The elevation of AMP/ATP ratio in cells activates cellular energetic sensor, AMPK, leading to the suppression of anabolism and promotion of catabolism [47][48][49][50] . We identified that eBAG treatment depleted cellular bioenergetics and resulted in AMPK activation.…”

Section: Discussionmentioning

confidence: 99%

3-Epi-betulinic acid 3- O- β- D-glucopyranoside (eBAG) induces autophagy by activation of AMP-activated protein kinase in hepatocellular carcinoma

Liu¹,

Liu²,

He³

et al. 2024

Food Science and Human Wellness

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Epi-betulinic acid 3-O-β-D-glucopyranoside (eBAG) is a pentacyclic triterpene mainly distributed in food and medicinal plants, which exhibits various pharmacological properties. However, whether these functions are attributed to eBAG or additional components in these plants remain unknown. Herein, we report that eBAG exerted an inhibitory activity against hepatocellular carcinoma and esophageal cancer cells. EBAG induced non-apoptotic cell death in hepatocellular carcinoma cells. The eBAG-induced cell death was inhibited by knock-down of autophagy related gene (ATG) 5 and ATG7, by administration of 3-methyladenine, a selective autophagy inhibitor that suppresses phosphoinositide 3-kinase (PI3K), and by chloroquine, a classic autophagy fl ux inhibitor. We demonstrated that eBAG induced an autophagy-mediated cell death. Application of eBAG mimicked cellular bioenergetics depletion leading to the reduction of intracellular ATP, activation of AMP-activated protein kinase (AMPK), and inhibition of mTOR. Co-treatment with compound C, an AMPK inhibitor, abrogated cell death induced by eBAG. We further validated the anti-tumor effect of eBAG in the murine xenograft model of hepatocellular carcinoma and found that eBAG treatment promoted the induction of autophagy and reduction of tumor growth in mice. As a functional food ingredient, eBAG is a potential therapeutic agent for the treatment of hepatocellular carcinoma and esophageal cancer.

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