An acyl-CoA-binding protein from grape that is induced through ER stress confers morphological changes and disease resistance in Arabidopsis

Takato, Haruka; Shimidzu, Masafumi; Ashizawa, Yuta; Takei, Hiroki; Suzuki, Shunji

doi:10.1016/j.jplph.2012.11.011

Cited by 23 publications

(24 citation statements)

References 42 publications

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“…Previous work on transgenic Arabidopsis overexpressing AtACBP3 showed that they conferred resistance only to a bacterial biotroph P. syringae accompanied by increased susceptibility to a fungal necrotroph B. cinerea . Earlier reports illustrated that transgenic Arabidopsis overexpressing a grapevine (dicot) Class III ACBP, V. vinifera ACBP , protected against the biotroph P. syringae and a hemibiotroph C. higginsianum , suggesting that both dicot and monocot Class III ACBPs are promising tools for application in disease protection of crop plants.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the overexpression in Arabidopsis of a homologous Class III ACBP from the dicot Vitis vinifera (grapevine) was reported to improve resistance to P. syringae and C. higginsianum . As ACBPs from monocotyledonous plants such as rice (Oryza sativa) are least explored in comparison with ACBPs from dicotyledonous plants, rice ACBP5 (OsACBP5) which represents the homolog of AtACBP3, was selected for investigations herein. Among the six OsACBPs , only OsACBP5 expression was induced upon infection with the hemibiotrophic rice blast fungal pathogen, Magnaporthe grisea .…”

Section: Introductionmentioning

confidence: 99%

“…Among the six OsACBPs , only OsACBP5 expression was induced upon infection with the hemibiotrophic rice blast fungal pathogen, Magnaporthe grisea . Given that the overexpression of dicot Class III ACBPs in Arabidopsis had conferred tolerance to pathogen infection, it would be pertinent to evaluate the response of a monocot ACBP such as OsACBP5 in transgenic Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of a Monocot Acyl‐CoA‐Binding Protein Confers Broad‐Spectrum Pathogen Protection in a Dicot

et al. 2019

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Plants are continuously infected by various pathogens throughout their lifecycle. Previous studies have reported that the expression of Class III acyl-CoA-binding proteins (ACBPs) such as the Arabidopsis ACBP3 and rice ACBP5 were induced by pathogen infection. Transgenic Arabidopsis AtACBP3-overexpressors (AtACBP3-OEs) displayed enhanced protection against the bacterial biotroph, Pseudomonas syringae, although they became susceptible to the fungal necrotroph Botrytis cinerea. A Class III ACBP from a monocot, rice (Oryza sativa) OsACBP5 was overexpressed in the dicot Arabidopsis. The resultant transgenic Arabidopsis lines conferred resistance not only to the bacterial biotroph P. syringae but to fungal necrotrophs (Rhizoctonia solani, B. cinerea, Alternaria brassicicola) and a hemibiotroph (Colletotrichum siamense). Changes in protein expression in R. solani-infected Arabidopsis OsACBP5-overexpressors (OsACBP5-OEs) were demonstrated using proteomic analysis. Biotic stress-related proteins including cell wallrelated proteins such as FASCILIN-LIKE ARABINOGALACTAN-PROTEIN10, LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEINS, XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN4, and PECTINESTERASE INHIBITOR18; proteins associated with glucosinolate degradation including GDSL-LIKE LIPASE23, EPITHIOSPECIFIER MODIFIER1, MYROSINASE1, MYROSINASE2, and NITRILASE1; as well as a protein involved in jasmonate biosynthesis, ALLENE OXIDE CYCLASE2, were induced in OsACBP5-OEs upon R. solani infection. These results indicated that upregulation of these proteins in OsACBP5-OEs conferred protection against various plant pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Overexpression of a Monocot Acyl‐CoA‐Binding Protein Confers Broad‐Spectrum Pathogen Protection in a Dicot

et al. 2019

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“…In plants, ACBPs are involved in many stages of plant growth and development, including flowering, embryo development, seed germination and seedling development [27]. Arabidopsis overexpressing grapevine VvACBP1 shown slowed-down floral transition by suppressing the photoperiodic pathway [38]. During embryogenesis of Arabidopsis, AtACBP1 and AtACBP2 were involved in the synthesis of phospholipid and galactolipid in ER [39].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization, expression and functional analysis of acyl-CoA-binding protein gene family in maize (Zea mays)

Zhu

Zhou

et al. 2019

Preprint

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Background: Acyl-CoA-binding proteins (ACBPs) have a conserved acyl-CoA-binding (ACB) domain which facilitates binding to the acyl-CoA ester and trafficking in eukaryotic cells. Although the various functions of ACBP have been characterized in several plant species, its structural features, molecular evolution, expression profiling and function in Zea mays L . are still not clear. Results: Genome-wide analysis identified nine ZmACBP genes in Z. mays , which were divided into four groups (class I, class II, class III, and class IV) by a phylogenetic tree constructed with 48 ACBP genes from six plant species. The subcellular localizations of the ZmACBPs were displayed subcellular multi-localization via transient expression of green fluorescence protein (GFP) fusions in tobacco ( Nicotiana tabacum ) epidermal cells. The expression profiles of ZmACBPs are dramatically different in different organs and normal growth, as well as abiotic or biotic stress by Quantitative RT-PCR (qRT-PCR). Eight of nine ZmACBP genes located in metaQTL regions associated with agronomic traits in maize. The heterologous constitutive expression of ZmACBP1 and ZmACBP3 in Arabidopsis enhanced the resistance to salinity and osmotic stress. Conclusion: The ACBP genes family in Z. mays were highly conserved. ZmACBP genes had clear tissue and organ expression preference and were involved in abiotic or biotic stress response, suggesting their roles in plant growth and stress resistance.

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“…Thus far, the functions of plant ACBPs have been widely elucidated in the eudicots, such as Arabidopsis and Brassica napus , although there have been many reports on the characterization of ACBP family members from other plant species, such as Oryza sativa , Agave americana , Vernicia fordii , Vitis vinifera , Helianthus annuus , Gossypium hirsutum , Jatropha curcas , and Elaeis guineensis (Du et al 2016 ; Raboanatahiry et al 2018 ; Liao et al 2019 ; Amiruddin et al 2020 ). Other than Arabidopsis and Brassica napus , the functions of ACBPs from other plant species have been mostly investigated using transgenic Arabidopsis (Takato et al 2013 ; Meng et al 2014 ; Panthapulakkal Narayanan et al 2019 ). Previously, we have reported a peroxisome-localized rice Class IV ACBP, OsACBP6, which is currently the only plant ACBP identified to be localized at the peroxisome (Meng et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice

Meng

et al. 2020

Rice

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Backgrounds Acyl-coenzyme A (CoA) esters are important intermediates in lipid metabolism with regulatory properties. Acyl-CoA-binding proteins bind and transport acyl-CoAs to fulfill these functions. RICE ACYL-COA-BINDING PROTEIN6 (OsACBP6) is currently the only one peroxisome-localized plant ACBP that has been proposed to be involved in β-oxidation in transgenic Arabidopsis. The role of the peroxisomal ACBP (OsACBP6) in rice (Oryza sativa) was investigated. Results Here, we report on the function of OsACBP6 in rice. The osacbp6 mutant showed diminished growth with reduction in root meristem activity and leaf growth. Acyl-CoA profiling and lipidomic analysis revealed an increase in acyl-CoA content and a slight triacylglycerol accumulation caused by the loss of OsACBP6. Comparative transcriptomic analysis discerned the biological processes arising from the loss of OsACBP6. Reduced response to oxidative stress was represented by a decline in gene expression of a group of peroxidases and peroxidase activities. An elevation in hydrogen peroxide was observed in both roots and shoots/leaves of osacbp6. Taken together, loss of OsACBP6 not only resulted in a disruption of the acyl-CoA homeostasis but also peroxidase-dependent reactive oxygen species (ROS) homeostasis. In contrast, osacbp6-complemented transgenic rice displayed similar phenotype to the wild type rice, supporting a role for OsACBP6 in the maintenance of the acyl-CoA pool and ROS homeostasis. Furthermore, quantification of plant hormones supported the findings observed in the transcriptome and an increase in jasmonic acid level occurred in osacbp6. Conclusions In summary, OsACBP6 appears to be required for the efficient utilization of acyl-CoAs. Disruption of OsACBP6 compromises growth and led to provoked defense response, suggesting a correlation of enhanced acyl-CoAs content with defense responses.

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An acyl-CoA-binding protein from grape that is induced through ER stress confers morphological changes and disease resistance in Arabidopsis

Cited by 23 publications

References 42 publications

Overexpression of a Monocot Acyl‐CoA‐Binding Protein Confers Broad‐Spectrum Pathogen Protection in a Dicot

Overexpression of a Monocot Acyl‐CoA‐Binding Protein Confers Broad‐Spectrum Pathogen Protection in a Dicot

Molecular characterization, expression and functional analysis of acyl-CoA-binding protein gene family in maize (Zea mays)

RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice

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