An Agglutinin Associated with the P and the ABO Blood Group Systems

Tippett, Patricia; Sanger, Ruth; Race, R. R.; Swanson, Jane L.; Busch, Shirley

doi:10.1111/j.1423-0410.1965.tb01390.x

Cited by 41 publications

(32 citation statements)

References 4 publications

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“…However, the pattern of the score distribution in this study is very similar to that reported by Tippett et al [1], whereas a study based on the monoclonal antibody MC813-70 (anti-SSEA-4) did not quite reach this conclu sion [2). The obviously increased number of weakly LKE-positive donors in group A as compared to group 0 might reflect an interference on the level of synthesis or presentation between the ABO and Luke systems rather than a linkage disequili brium on a genetic basis.…”

Section: Discussionsupporting

confidence: 60%

“…The previously described association of LKE and P| [1] was confirmed, and seemed restricted to group A donors. The anti-LKE serum also contained anti-P| reactivity.…”

Section: Discussionmentioning

confidence: 60%

“…The Luke (LKE) phenotype of human erythrocytes was described in 1965 by Tip pett et al [1], LKE+ is very common in Caucasians, but few studies have been done to determine the pheno-or genotype frequencies. A monoclonal antibody (antiStage Specific Embryonic Antigen-4) against LKE antigens has been made and used by Tippett et al [2] to determine the LKE phenotype frequency in an English donor population.…”

Section: Introductionmentioning

confidence: 99%

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Phenotype Frequency of LKE in the Danish Population

Møller

Jørgensen²

1988

Hum Hered

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A panel of 897 randomly chosen Danish donors of blood groups 0 and A was tested for the LKE antigen using a human anti-LKE. In this sample, 78.7% were LKE positive, 20.6% were weakly positive, whereas 0.7% were LKE negative. These phenotype frequencies are similar to those obtained by the first discovered human alloserum and by the monoclonal anti-SSEA-4 in previous studies. The previously reported lower LKE antigen strength in group A versus group 0 donors, and the association of LKE to the P system was confirmed.

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Section: Discussionsupporting

confidence: 60%

“…The previously described association of LKE and P| [1] was confirmed, and seemed restricted to group A donors. The anti-LKE serum also contained anti-P| reactivity.…”

Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

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Phenotype Frequency of LKE in the Danish Population

Møller

Jørgensen²

1988

Hum Hered

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“…Based on MAb MC813-70 staining, SSEA-4 has been identified in undifferentiated human embryonic carcinoma cells and seminomas (30). Agglutination of papain-treated human erythrocytes also occurs with MAb MC813-70, identifying the Luke antigen (38,39), but the molecule on which the Luke antigen is carried on erythrocytes has not been isolated and structurally characterized. Thus, the antibody staining data previously reported by Karr et al suggested, but did not prove, that SGG was expressed in human kidney tissue.…”

Section: Discussionmentioning

confidence: 99%

The Globoseries Glycosphingolipid Sialosyl Galactosyl Globoside is Found in Urinary Tract Tissues and is a Preferred Binding Receptor In Vitro for Uropathogenic Escherichia Coli Expressing Pap-Encoded Adhesins

et al. 1999

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Women with a history of recurrent Escherichia coli urinary tract infections (UTIs) are significantly more likely to be nonsecretors of blood group antigens than are women without such a history, and vaginal epithelial cells (VEC) from women who are nonsecretors show enhanced adherence of uropathogenic E. coli isolates compared with cells from secretors. We previously extracted glycosphingolipids (GSLs) from native VEC and determined that nonsecretors (but not secretors) selectively express two extended globoseries GSLs, sialosyl galactosyl globoside (SGG) and disialosyl galactosyl globoside (DSGG), which specifically bound uropathogenic E. coli R45 expressing a P adhesin. In this study, we demonstrated, by purifying the compounds from this source, that SGG and DSGG are expressed in human kidney tissue. We also demonstrated that SGG and DSGG isolated from human kidneys bind uropathogenic E. coli isolates expressing each of the three classes of pap-encoded adhesins, including cloned isolates expressing PapG from J96, PrsG from J96, and PapG from IA2, and the wild-type isolates IA2 and R45. We metabolically 35 S labeled these five E. coli isolates and measured their relative binding affinities to serial dilutions of SGG and DSGG as well as to globotriaosylceramide (Gb 3 ) and globotetraosylceramide (Gb 4 ), two other globoseries GSLs present in urogenital tissues. Each of the five E. coli isolates bound to SGG with the highest apparent avidity compared with their binding to DSGG, Gb 3 , and Gb 4 , and each isolate had a unique pattern of GSL binding affinity. These studies further suggest that SGG likely plays an important role in the pathogenesis of UTI and that its presence may account for the increased binding of E. coli to uroepithelial cells from nonsecretors and for the increased susceptibility of nonsecretors to recurrent UTI.Several epidemiological studies have shown that women who are nonsecretors of blood group antigens have a three-to fourfold-increased risk of developing recurrent urinary tract infection (UTI) (5,17,32). In addition, uroepithelial cells from nonsecretors have a two-to threefold-greater capacity for adherence of uropathogenic Escherichia coli than do cells from secretors (22). Colonization of the vaginal and periurethral epithelium precedes the development of E. coli UTI, and E. coli isolates expressing pap-encoded adhesins are overrepresented among isolates causing these infections (6). Uropathogenic E. coli isolates expressing pap-encoded adhesins bind to globoseries glycosphingolipids (GSLs) (6, 19), which are amphipathic molecules embedded in the outer leaflets of eukaryotic cell membranes. There are several families of GSLs which are differentiated by their molecular structures, and these molecules serve as bacterial and viral adhesion sites on mammalian cells and as markers of eukaryotic cell differentiation and oncogenesis (4).In previous investigations, we collected vaginal epithelial cells from secretors and nonsecretors and extracted the GSLs from pooled cells from women in eac...

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“…In contrast, normal P+, LKEN donors still have low levels of MSGG when examined by sensitive methods [3,4]. Despite the incidence of LKEN in the general population, examples of antiLKE are quite rare, with only six examples mentioned in the literature [1,59]. We had the opportunity to reexamine a historical 'anti LKE' from an untransfused, group A, P2, 32 years old woman in her second pregnancy [5,6].…”

Section: Original Article Open Accessmentioning

confidence: 99%