An Amperometric Glucose Biosensor Based on Titania Sol-gel/Prussian Blue Composite Film

Abstract

An improved amperometric glucose biosensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared by a vapor deposition method, on a Prussian Blue (PB)-modified electrode. The method combined the merits of immobilizing biomolecules in the titania sol-gel film by vapor deposition method and the synergic catalysis effects of PB and GOD molecules. Results showed that the fabricated titania sol-gel/PB membrane possessed high surface area, good mechanical stability, and good hydrophilicity, which provided a biocompatible microenvironment for maintaining the bioactivity of the immobilized enzyme and prevented the enzyme from leaking out of the film. Therefore, the present biosensor exhibited fast response time (10 s), high sensitivity (12.74 mA cm -2 mM -1 ), long-term operational stability, good suppression of interference, and a wide linear range from 0.02 to 15 mM with a low detection limit of 5 mM for the detection of glucose. In addition, this simple and controllable method could fabricate biosensors in batches with a very small amount of enzyme.

A contact lens with embedded sensor for monitoring tear glucose level

“…The unused sensor can retain its activity for one week if stored in buffer at 4 °C directly after preparation. The long lifetime is because the sol-gel film can prevent GOD from leaking out and the immobilization process does not influence the biological activity of enzyme, as reported (Yu et al, 2003; Liang et al, 2008). …”

“…Considering the small area of the working electrode (about 0.22 mm 2 ), the sensitivity of our sensor is about 240 µAcm −2 mM −1 , higher than conventional disc electrodes with sol-gel film (Yu et al, 2003; Liang et al, 2008), and nanotube array sensors (Ekanavake et al, 2007). The good performance is due to both the micro-scale electrode design and the titania sol-gel film.…”

One‐step preparation and application of mussel‐inspired poly(norepinephrine)‐coated polydimethylsiloxane microchip for separation of chiral compounds

“…PDMS microchip with a simple cross‐configuration was fabricated as previously described procedure . Briefly, PDMS oligomer and curing agent were thoroughly mixed at a ratio of 10:1 w/w, degassed, and then poured on the SU‐8 2035 photoresist molding.…”

Titanium Dioxide Nanomaterials for Sensor Applications

“…It is well biocompatible and can retain the catalytic activities of biomolecule to a large extent. Various sol–gel made TiO 2 materials have been integrated into work electrode with binding molecules or polymer networks to form crack-free porous structures and have been characterized and applied to biosensing. − Yu et al developed an amperometric H 2 O 2 sensor by immobilizing horseradish peroxidase (HRP) in the TiO 2 film made by sol–gel method. When applied at −150 mV in the PBS/catechol electrolyte, the reduced form of catechol reacted further with the oxidized form of HRP to form reduced HRP, which catalyzes the reduction of H 2 O 2 .…”