An Antifungal Exo-α-1,3-Glucanase (AGN13.1) from the Biocontrol FungusTrichoderma harzianum

Abstract

Trichoderma harzianum secretes α-1,3-glucanases when it is grown on polysaccharides, fungal cell walls, or autoclaved mycelium as a carbon source (simulated antagonistic conditions). We have purified and characterized one of these enzymes, named AGN13.1. The enzyme was monomeric and slightly basic. AGN13.1 was an exo-type α-1,3-glucanase and showed lytic and antifungal activity against fungal plant pathogens. Northern and Western analyses indicated that AGN13.1 is induced by conditions that simulated antagonism. We propose that AGN13.1 contributes to the antagonistic response of T. harzianum.

Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride

“…The mechanism of mycoparasitism involves nutrient competition, hyperparasitism and antibiosis (Ajith and Lakshmidevi 2010). Recently, the role of extracellular enzymes has been well documented by several researchers such as proteolytic enzymes (Pozo et al 2004; Kredics et al 2005), β-1,3-glucanolytic system (Kubicek et al 2001), β-1,6-glucanases (De La Cruz et al 1995; De La Cruz and Llobell 1999), α-1,3-glucanases (Ait-Lahsen et al 2001), chitinase (Zeilinger et al 1999; Hoell et al 2005) and proteases (Geremia et al 1993), which are considered as key factors in the pathogen cell wall lysis during mycoparasitism (Brito-Vega et al 2013). …”

Cloning and Expression of an α-1,3-Glucanase Gene fromBacillus circulansKA-304: The Enzyme Participates in Protoplast Formation ofSchizophyllum commune

“…28,29) -1,3-Glucanases of T. harzianum and T. asperellum (glycosyl hydrolase family 71), 28,29) which secrete when the organisms grow on the fungal cell-wall as a carbon source, have been investigated in detail, and the enzymes showed lytic and antifungal activity against fungal plant pathogens. The enzyme of Trichoderma strains is also a modular enzyme, composed of an N-terminal catalytic domain, a proline-serine-threoninerich linker domain, and a C-terminal -1,3-glucan binding domain (carbohydrate-binding module family 24).…”

“…The presence of mutanases has been also reported in several bacteria [14][15][16][17][18][19][20][21][22] and filamentous fungi. [23][24][25][26][27][28][29][30][31][32][33] Among these -1,3-glucanases, the genes of y To whom correspondence should be addressed. Tel: +81-77-566-1111 ex.…”

Evaluación de hongos antagonistas de <i>botrytis cinerea</i> pers., en plantaciones de mora, Costa Rica

“…También se atribuye este efecto a enzimas que interfieren con el desarrollo del patógeno porque degradan componentes estructurales de la pared celular, como las quitinasas, exo-y endoglucanasas y proteasas principalmente producidas por T. harzianum (Elad y Kapat 1999, Ait-Lahsen et al 2001.…”