An aptasensor based on heparin-mimicking hyperbranched polyester with anti-biofouling interface for sensitive thrombin detection

Niu, Yanlian; Chu, Meilin; Xu, Ping; Meng, Shuangshuang; Zhou, Qian; Zhao, Wenbo; Zhao, Bo; Shen, Jian

doi:10.1016/j.bios.2017.10.037

Cited by 26 publications

(16 citation statements)

References 41 publications

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“…For both research and clinical diagnosis purpose, detection of thrombin is very important because thrombin plays a central role in hemostasis and hemolysis. While different approaches have been demonstrated for thrombin detection at low concentrations, we propose a simple and scalable technique for label‐free optical detection of thrombin at ultralow concentrations. Since graphene can selectively adsorb aromatic rings structure biomolecules such as DNA, RNA, peptides, and cytokines through pi‐stacking forces and Sb 2 S 3 –TiN HMM shows enhanced GH shifts at visible wavelengths, the proposed configuration is a unique platform for thrombin detection at ultralow concentrations.…”

mentioning

confidence: 99%

Phase‐Change‐Material‐Based Low‐Loss Visible‐Frequency Hyperbolic Metamaterials for Ultrasensitive Label‐Free Biosensing

Sreekanth

Ouyang

Sreejith

et al. 2019

Advanced Optical Materials

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The extreme anisotropic permittivity of hyperbolic metamaterials (HMMs) represent a unique opportunity to realize effective bulk metastructures with extraordinary optical properties over a broad frequency range from visible to terahertz. [1] HMMs are artificial uniaxial materials that exhibit hyperbolic dispersion because the out of plane dielectric constant (ε zz = ε ⊥ ) has an opposite sign to the in-plane dielectric constants (ε xx = ε yy = ε ll ). HMMs can be classified into two types based on the sign of their dielectric components, i.e., type I (−ε ⊥ and ε ll ) and type II (ε ⊥ and −ε ll ). In comparison to isotropic materials showing elliptical dispersion, HMMs support propagation of optical modes across the structure with infinitely large momentum (high-k modes) in the effective medium limit, [2,3] irrespective of Hyperbolic metamaterials (HMMs) have emerged as a burgeoning field of research over the past few years as their dispersion can be easily engineered in different spectral regions using various material combinations. Even though HMMs have comparatively low optical loss due to a single resonance, the noble-metal-based HMMs are limited by their strong energy dissipation in metallic layers at visible frequencies. Here, the fabrication of noble-metal-free reconfigurable HMMs for visible photonic applications is experimentally demonstrated. The low-loss and active HMMs are realized by combining titanium nitride (TiN) and stibnite (Sb 2 S 3 ) as the phase change material. A reconfigurable plasmonic biosensor platform based on active Sb 2 S 3 -TiN HMMs is proposed, and it is shown that significant improvement in sensitivity is possible for small molecule detection at low concentrations. In addition, a plasmonic apta-biosensor based on a hybrid platform of graphene and Sb 2 S 3 -TiN HMM is developed and the detection and real-time binding of thrombin concentration as low as 1 × 10 −15 m are demonstrated. A biosensor operating in the visible range has several advantages including the availability of sources and detectors in this region, and ease of operation particularly for point-of-care applications.

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mentioning

confidence: 99%

Phase‐Change‐Material‐Based Low‐Loss Visible‐Frequency Hyperbolic Metamaterials for Ultrasensitive Label‐Free Biosensing

Sreekanth

Ouyang

Sreejith

et al. 2019

Advanced Optical Materials

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“…The use of a heparin-mimicking hyperbranched polyester nanoparticle based aptasensor for the detection of thrombin in whole blood has been explored by Niu et al 170 As shown in Figure 19A, the proposed sensor interface was obtained through a layer-by-layer self-assembly approach, with 3mercaptopropyltrimethoxysilane (MPTMS) first grafted onto the surface of GC to enable Au NPs anchoring. Then hyperbranched polyester-sulfonic acid nanoparticles (HBPE-SO 3 NPs) were attached via electrostatic adsorption, followed by immobilization of a 5′-amino thrombin aptamer by physisorption.…”

Section: Sensors Based On Chemical Antifouling Interfacesmentioning

confidence: 99%

“…(A) Schematic illustration of the fabrication of a hyperbranched polyester-sulfonic acid nanoparticle-based aptasensor for electrochemical sensing of thrombin in undiluted serum. Reproduced with permission from ref . Copyright 2017 Elsevier.…”

Section: Chemical Antifouling Strategies and Their Sensing Applicationsmentioning

confidence: 99%

Antifouling Strategies for Selective In Vitro and In Vivo Sensing

et al. 2020

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The ability to fabricate sensory systems capable of highly selective operation in complex fluid will undoubtedly underpin key future developments in healthcare. However, the abundance of (bio)molecules in these samples can significantly impede performance at the transducing interface where nonspecific adsorption (fouling) can both block specific signal (reducing sensitivity) and greatly reduce assay specificity. Herein, we aim to provide a comprehensive review discussing concepts and recent advances in the construction of antifouling sensors that are, through the use of chemical, physical, or biological engineering, capable of operating in complex sample matrix (e.g., serum). We specifically highlight a range of molecular approaches to the construction of solid sensory interfaces (planar and nanoparticulate) and their characterization and performance in diverse in vitro and in vivo analyte (e.g., proteins, nucleic acids, cells, neuronal transmitters) detection applications via derived selective optical or electrochemical strategies. We specifically highlight those sensors that are capable of detection in complex media or those based on novel architectures/approaches. Finally, we provide perspectives on future developments in this rapidly evolving field.

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“…Moreover, the sensor showed good specificity in response to other serum proteins and retained 92.8% of its signal after 20 days. In a more recent study by Niu et al [122], hyperbranched polyester was once again used as an anti-biofouling coating, this time in the form of heparin-mimicking hyperbranched polyester nanoparticles (HBPE-SO 3 NPs). HBPE-SO 3 NPs and positively charged Au NPs were used to modify a GCE and exhibited anticoagulant activity.…”

Section: Polymer-based Thrombin Electrochemical Aptasensorsmentioning

confidence: 99%

Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin

2022

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Thrombin plays a central role in hemostasis and its imbalances in coagulation can lead to various pathologies. It is of clinical significance to develop a fast and accurate method for the quantitative detection of thrombin. Electrochemical aptasensors have the capability of combining the specific selectivity from aptamers with the extraordinary sensitivity from electrochemical techniques and thus have attracted considerable attention for the trace-level detection of thrombin. Nanomaterials and nanostructures can further enhance the performance of thrombin aptasensors to achieve high sensitivity, selectivity, and antifouling functions. In highlighting these material merits and their impacts on sensor performance, this paper reviews the most recent advances in label-free electrochemical aptasensors for thrombin detection, with an emphasis on nanomaterials and nanostructures utilized in sensor design and fabrication. The performance, advantages, and limitations of those aptasensors are summarized and compared according to their material structures and compositions.

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An aptasensor based on heparin-mimicking hyperbranched polyester with anti-biofouling interface for sensitive thrombin detection

Cited by 26 publications

References 41 publications

Phase‐Change‐Material‐Based Low‐Loss Visible‐Frequency Hyperbolic Metamaterials for Ultrasensitive Label‐Free Biosensing

Phase‐Change‐Material‐Based Low‐Loss Visible‐Frequency Hyperbolic Metamaterials for Ultrasensitive Label‐Free Biosensing

Antifouling Strategies for Selective In Vitro and In Vivo Sensing

Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin

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