An assessment of the transmission rate of four pospiviroid species through tomato seeds

Faggioli, F.; Luigi, M.; Šveikauskas, Vaidevutis; Thibault, Olivier; Marn, M. Viršček; Pleško, Irena Mavrič; Jonghe, Kris De; Bogaert, Noémi Van; Grausgruber-Gröger, Sabine

doi:10.1007/s10658-015-0707-7

Cited by 12 publications

(8 citation statements)

References 17 publications

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“…Viroids as non-coding "parasitic" RNAs [12,13] show fast evolutionary changes during the adaptation to new hosts, and their spreading is intensively facilitated by the agrotechnique (predominantly mechanical transmission) [12]. Some viroids are transmissible through plant generative phase, pollen and seeds [14][15][16][17][18][19][20][21][22][23]. Transmission routes via seeds and pollen are the major ways of infection propagation from parental plants to their progeny and distribution among individuals.…”

Section: Introductionmentioning

confidence: 99%

Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes

Matoušek

Steinbachová

Drábková

et al. 2020

IJMS

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Some viroids—single-stranded, non-coding, circular RNA parasites of plants—are not transmissible through pollen to seeds and to next generation. We analyzed the cause for the elimination of apple fruit crinkle viroid (AFCVd) and citrus bark cracking viroid (CBCVd) from male gametophyte cells of Nicotiana tabacum by RNA deep sequencing and molecular methods using infected and transformed tobacco pollen tissues at different developmental stages. AFCVd was not transferable from pollen to seeds in reciprocal pollinations, due to a complete viroid eradication during the last steps of pollen development and fertilization. In pollen, the viroid replication pathway proceeds with detectable replication intermediates, but is dramatically depressed in comparison to leaves. Specific and unspecific viroid degradation with some preference for (−) chains occurred in pollen, as detected by analysis of viroid-derived small RNAs, by quantification of viroid levels and by detection of viroid degradation products forming “comets” on Northern blots. The decrease of viroid levels during pollen development correlated with mRNA accumulation of several RNA-degrading factors, such as AGO5 nuclease, DICER-like and TUDOR S-like nuclease. In addition, the functional status of pollen, as a tissue with high ribosome content, could play a role during suppression of AFCVd replication involving transcription factors IIIA and ribosomal protein L5.

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Section: Introductionmentioning

confidence: 99%

Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes

Matoušek

Steinbachová

Drábková

et al. 2020

IJMS

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“…For citrus exocortis viroid (CEVd), Semancik [ 35 ] reported seed transmission in tomato without further details, whereas Faggioli et al [ 21 ] did not find transmission for 1849 seedlings. For CLVd, Matsushita and Tsuda [ 33 ] reported transmission for 46 out of 793 seedlings, whereas no seed transmission was found by Fox and Monger [ 36 ] and Faggioli et al [ 21 ] for 200 and 1599 seedlings. For PCFVd, Yanagisawa and Matsushita [ 32 ] reported three infected seedlings out of 941.…”

Section: Resultsmentioning

confidence: 99%

“…However, reports on seed transmission in these crops are contradictory. Under experimental conditions, substantial transmission rates, as well as the complete absence of seed transmission, have been reported, e.g., [ 21 , 22 ]. For outbreaks in commercial crops, some were considered to result from infested seeds [ 23 , 24 ], whereas for others, no evidence was found for seed as a source of infection [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Possible Overestimation of Seed Transmission in the Spread of Pospiviroids in Commercial Pepper and Tomato Crops Based on Large-Scale Grow-Out Trials and Systematic Literature Review

Verhoeven¹,

Botermans²,

Schoen³

et al. 2021

Plants

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Several outbreaks of pospiviroids have been reported in pepper and tomato crops worldwide. Tracing back the origin of the infections has led to different sources. In some cases, the infections were considered to result from seed transmission. Other outbreaks were related to transmission from ornamental crops and weeds. Pospiviroids, in particular potato spindle tuber viroid, are regulated by many countries because they can be harmful to potatoes and tomatoes. Seed transmission has been considered an important pathway of introduction and spread. However, the importance of this pathway can be questioned. This paper presents data on seed transmission from large-scale grow-out trials of infested pepper and tomato seed lots produced under standard seed-industry conditions. In addition, it presents the results of a systematic review of published data on seed transmission and outbreaks in commercial pepper and tomato crops. Based on the results of the grow-out trials and review of the literature, it was concluded that the role of seed transmission in the spread of pospiviroids in practice is possibly overestimated.

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“…Viroids are distinguished on the basis of their transmission within host plants following either vertical or horizontal patterns [ 57 ]. Pollen and ovules of a plant are responsible for transmission of viroid infections from parental plants through seeds and then seedlings to the next generation in the vertical mode of transmission; an example is the PSTVd.…”

Section: Biology Of Viroidsmentioning

confidence: 99%

An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids

Venkataraman¹,

Badar²,

Hashim³

et al. 2021

IJMS

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An assessment of the transmission rate of four pospiviroid species through tomato seeds

Cited by 12 publications

References 17 publications

Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes

Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes

Possible Overestimation of Seed Transmission in the Spread of Pospiviroids in Commercial Pepper and Tomato Crops Based on Large-Scale Grow-Out Trials and Systematic Literature Review

An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids

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