An autologous platelet-rich plasma hydrogel compound restores left ventricular structure, function and ameliorates adverse remodeling in a minimally invasive large animal myocardial restoration model: A translational approach

Vu, Thang Duc; Pal, Shripad N.; Ti, Lian Kah; Martinez, Eliana C.; Rufaihah, Abdul Jalil; Ling, Lieng H.; Lee, Chuen Neng; Richards, A. Mark; Kofidis, Theo

doi:10.1016/j.biomaterials.2014.12.013

Cited by 43 publications

(35 citation statements)

References 39 publications

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“…Released growth factors activate a cascade of signals leading to tissue repair (Nurden, ). Autologous PRP gel has been applied to promote tissue regeneration and repair in different surgical fields, such as dentistry, orthopaedics, plastic surgery and cosmetics (Alsousou et al , ; Dold et al , ; Intini, ; Martínez‐Zapata et al , ; Marx et al , ; Vu et al , ; Zheng et al , ). In particular, randomized controlled trials were performed to evaluate the effectiveness of PRP gel for the treatment of chronic and diabetic ulcers (Driver et al , ; Greer et al , ; Martínez‐Zapata et al , ).…”

Section: Discussionmentioning

confidence: 99%

“…Since then, several clinicians have adopted the autologous PRP to stimulate the repair of different tissue damages. These include chronic skin ulcers (Martínez‐Zapata et al , ), articular cartilage damage (Dold et al , ), bone and tendon lesions (Alsousou et al , ; Intini, ), peripheral nerve wound (Zheng et al , ), cardiac muscle injury (Vu et al , ), and plastic and maxillo‐facial applications (Martínez‐Zapata et al , ; Marx et al , ). PRP has also been used for the treatment of injuries in professional sportspersons (Dold et al , ; Taylor et al , ).…”

Section: Introductionmentioning

confidence: 99%

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Platelet-rich plasma-based bioactive membrane as a new advanced wound care tool

Spanò

Muraglia

Todeschi

et al. 2017

J Tissue Eng Regen Med

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Chronic skin ulcers, consequence of diabetes and other pathological conditions, heavily compromise the patient life quality and represent a high and constantly growing cost for National Health Services. Autologous platelet-rich plasma (PRP), has been proposed to treat these lesions. The absence of guidelines for the PRP production and the need of a fresh preparation for each treatment lead us to develop a protocol for the production of an allogenic PRP-based bioactive membrane (BAM), standardized for platelet concentration and growth factor release. This work compares BAMs obtained starting from two different platelet concentrations. There was no direct correlation between the amount of growth factors released by BAM in vitro and the initial platelet count. However, different release kinetics were noticed for different growth factors, suggesting that they were differently retained by the two BAMs. The angiogenic potential of both BAMs was determined by Luminex Angiogenesis Assay. The biological activity of the factors released by the two BAMs was confirmed by cell proliferation and migration. A diabetic mouse chronic ulcer model was used to define the best PRP therapeutic dose in vivo. Both BAMs induced wound healing by increasing the thickness of the regenerated epidermis and the vessel number. However, a too high platelet concentration resulted in a slowdown of the membrane resorption that interfered with the skin healing. Overall, the results indicate that the BAMs could represent a natural and effective wound healing tool for the treatment of skin ulcers. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma-based bioactive membrane as a new advanced wound care tool

Spanò

Muraglia

Todeschi

et al. 2017

J Tissue Eng Regen Med

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“…These include chronic skin ulcers (Martínez-Zapata et al, 2009), articular cartilage damage (Dold et al, 2014), bone and tendon lesions (Alsousou et al, 2009; Intini, 2009), peripheral nerve wound (Zheng et al, 2014), cardiac muscle injury (Vu et al, 2015), plastic and maxillofacial applications (Marx et al, 1998; Martínez-Zapata et al, 2009). PRP has also been used for the treatment of injuries in professional sportspersons (Taylor et al, 2011; Dold et al, 2014) and in racing animals (Waselau et al, 2008; Witte et al, 2016).…”

Section: Endogenous Regenerationmentioning

confidence: 99%

Learning from Mother Nature: Innovative Tools to Boost Endogenous Repair of Critical or Difficult-to-Heal Large Tissue Defects

Cancedda

Descalzi

Mastrogiacomo

et al. 2017

Front. Bioeng. Biotechnol.

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For repair of chronic or difficult-to-heal tissue lesions and defects, major constraints exist to a broad application of cell therapy and tissue engineering approaches, i.e., transplantation of “ex vivo” expanded autologous stem/progenitor cells, alone or associated with carrier biomaterials. To enable a large number of patients to benefit, new strategies should be considered. One of the main goals of contemporary regenerative medicine is to develop new regenerative therapies, inspired from Mother Nature. In all injured tissues, when platelets are activated by tissue contact, their released factors promote innate immune cell migration to the wound site. Platelet-derived factors and factors secreted by migrating immune cells create an inflammatory microenvironment, in turn, causing the activation of angiogenesis and vasculogenesis processes. Eventually, repair or regeneration of the injured tissue occurs via paracrine signals activating, mobilizing or recruiting to the wound site cells with healing potential, such as stem cells, progenitors, or undifferentiated cells derived from the reprogramming of tissue differentiated cells. This review, largely based on our studies, discusses the identification of new tools, inspired by cellular and molecular mechanisms overseeing physiological tissue healing, that could reactivate dormant endogenous regeneration mechanisms lost during evolution and ontogenesis.

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“…However, the endogenous antioxidant defense systems may fail to provide appropriate protection against oxidative stress in many acute and chronic CVD [17,18]. Therefore, exogenous application of antioxidants may be a potential treatment for CVD [19][20][21].…”

Section: Introductionmentioning

confidence: 98%