An Automated Method for Supporting Busulfan Therapeutic Drug Monitoring

Abstract

Availability of TDM of busulfan can be improved by the use of commercially available reagents and automated platforms.

Effects of Carob (Ceratonia siliqua) on Sperm Quality, Testicular Structure, Testosterone Level and Oxidative Stress in Busulfan-Induced Infertile Mice

“…1 Despite the many clinical applications of busulfan, it has most important complications which are spermatogenesis disorder and infertility. [2][3][4][5] Therefore, injections of various busulfan dosages are used for induction of infertility in animal models. [2][3][4][5] On the other hand, the administration of medicinal herbs with little side effects has a long history in treatment of infertility.…”

“…[2][3][4][5] Therefore, injections of various busulfan dosages are used for induction of infertility in animal models. [2][3][4][5] On the other hand, the administration of medicinal herbs with little side effects has a long history in treatment of infertility. Evidences show herbs such as ginsing, ginger, black seed and saffron are effective on male fertility.…”

A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography/tandem mass spectrometry

“…Recently, a simple LC/MS method for rapid measurement of Bu from plasma and serum has been described using a turbulent flow online extraction procedure . Simple measurement techniques such as immunoassay and automated photometric methods have also been developed to obtain quick and reliable results with good accuracy and precision comparable to the GC/MS method . Unlike GC/MS, LC, LC/MS/MS methods, immunoassay methods for Bu determination are not widely studied for their clinical utility.…”

Therapeutic Drug Monitoring of Busulfan for the Management of Pediatric Patients: Cross-Validation of Methods and Long-Term Performance

“…Bu TDM combined with model-based repeat-dose adjustment has been reported to be beneficial in children. 20,21 Several Bu quantification methods have been published using enzymelinked immunosorbent assay, 22,23 liquid chromatography with UV detection, 24 gas chromatography with mass spectrometry, 25 and liquid chromatography with tandem mass spectrometry (LC-MS/MS), 26,27 all with varying plasma volumes, sensitivities, and process durations. Because the estimation of PK parameters requires collecting repeated blood samples, taking small volumes is highly recommended for pediatric patients.…”