An automatic versatile system integrating solid-phase extraction with ultra-high performance liquid chromatography–tandem mass spectrometry using a dual-dilution strategy for direct analysis of auxins in plant extracts

Zhong, Qisheng; Qiu, Xiongxiong; Lin, Cai-Yong; Shen, Lingling; Huo, Yin; Zhan, Song; Yao, Jing; Huang, Taohong; Hashi, Yuki; Xiao, Langtao; Zhou, Ting

doi:10.1016/j.chroma.2014.07.031

Cited by 12 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the loading solvent that diluted the injected sample solution could greatly affect the extraction of SPE. The mixer 1 used in this on‐line system was a patent product of Shimadzu Corporation, which was a high‐efficient solvent mixer with 12 balls with inert surface inside . Several loading solvents were tested in this study: 5% v/v acetonitrile aqueous solution with 5 mM ammonium acetate, 5% v/v acetonitrile aqueous solution with 0.2% v/v formic acid, and water/acetonitrile (95:5, v/v).…”

Section: Resultsmentioning

confidence: 99%

“…The automatic analysis on‐line system coupling SPE with UHPLC–MS/MS was converted from the Shimadzu LC–MS/MS system (Shimadzu, Kyoto, Japan), which has been described elsewhere (shown in Fig. ).…”

Section: Methodsmentioning

confidence: 99%

“…Many different hyphenated SPE–LC on‐line systems have been developed, such as SPE–HPLC , SPE–LC–MS/MS , SPE–LC–TOF‐MS , and so on. In our previous study, an automatic analysis on‐line system coupling SPE with UHPLC–MS/MS using a dual‐dilution strategy was developed . In this system, diverse commercial SPE columns can be used under an ambient pressure.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Zhong

Shen

Liu

et al. 2016

J of Separation Science

Self Cite

View full text Add to dashboard Cite

An automatic on-line solid-phase extraction with ultra-high performance liquid chromatography and tandem mass spectrometry method was developed for the simultaneous determination of ten antipsychotics in human plasma. The plasma sample after filtration was injected directly into the system without any pretreatment. A Shim-pack MAYI-C8 (G) column was used as a solid-phase extraction column, and all the analytes were separated on a Shim-pack XR-ODS III column with a mobile phase consisting of 0.1% v/v formic acid in water with 5 mM ammonium acetate and acetonitrile. The method features were systematically investigated, including extraction conditions, desorption conditions, the equilibration solution, the valve switching time, and the dilution for column-head stacking. Under the optimized conditions, the whole analysis procedure took only 10 min. The limits of quantitation were in the range of 0.00321-2.75 μg/L and the recoveries ranged from 75.9 to 122%. Compared with the off-line ultra-high performance liquid chromatography and the reported methods, this validated on-line method showed significant advantages such as minimal pretreatment, shortest analysis time, and highest sensitivity. The results indicated that this automatic on-line method was rapid, sensitive, and reliable for the determination of antipsychotics in plasma and could be extended to other target analytes in biological samples.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Zhong

Shen

Liu

et al. 2016

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Currently, organic extraction and solid-phase extraction are the most frequently used methods in phytohormone extraction and purification prior to their analysis by LC-MS/MS. MIPs and some developed operational strategies, such as two-dimensional HPLC, online 2D HPLC and high-performance thin-layer chromatography (HPTLC), have been employed to purify phytohormones [23, 55, 56]. …”

Section: Discussionmentioning

confidence: 99%

Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

Luo

Chen

et al. 2017

Biol Proced Online

Self Cite

View full text Add to dashboard Cite

BackgroundIndole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE columns are commercially available at the moment. Therefore, the development of IAA-specific recognition materials and IAA extraction and purification methods will help researchers meet the need for more precise analytical methods for research on phytohormones.ResultsSince the AUXIN RESISTANT/INDOLE-3-ACETIC ACID INDUCIBLE (Aux/IAA) proteins show higher specific binding capability with auxin, recombinant IAA1, IAA7 and IAA28 proteins were used as sorbents to develop an IAA extraction and purification method. A GST tag was used to solidify the recombinant protein in a column. Aux/IAA proteins solidified in a column have successfully trapped trace IAA in aqueous solutions. The IAA7 protein showed higher IAA binding capability than the other proteins tested. In addition, expression of the IAA7 protein in Drosophila Schneider 2 (S2) cells produced better levels of binding than IAA7 expressed in E. coli.ConclusionThis work validated the potential of Aux/IAA proteins to extract and purify IAA from crude plant extracts once we refined the techniques for these processes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12575-016-0050-1) contains supplementary material, which is available to authorized users.

show abstract

“…FSS is a strategy based on online dividing a sample solution into fractions by plugs of weak mobile phase, followed by head‐column stacking process. The other configuration of automatic SPE‐UHPLC‐MS/MS system using a dual‐dilution strategy is shown in Figure C . The first dilution that takes place before SPE enables the direct injection of complex samples with minimal pretreatment, and the second dilution that takes place after SPE process and before the UHPLC procedure achieves the direct introduction of large volume of strong eluent into the UHPLC column without causing peak broadening or distortion.…”

Section: Online Sample Preparation‐uhplc Techniquesmentioning

confidence: 99%

Recent advances of online coupling of sample preparation techniques with ultra high performance liquid chromatography and supercritical fluid chromatography

Liang

Zhou

2018

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Ultra high performance liquid chromatography and supercritical fluid chromatography techniques are favored because of their high efficiency and fast analysis speed. Although many sample preparation techniques have been coupled with common liquid chromatography online, the online coupling of sample preparation with the two popular chromatography techniques have gained increasing attention owing to the increasing requirements of efficiency and sensitivity. In this review, we have discussed and summarized the recent advances of the online coupling of sample preparation with ultra high performance liquid chromatography and supercritical fluid chromatography techniques. The main sample preparation techniques that have been coupled with ultra high performance liquid chromatography online are solid-phase extraction and in-tube solid-phase microextraction, while solid-phase extraction and supercritical fluid extraction are the main techniques that have been coupled with supercritical fluid chromatography online. Especially, the strategies for online coupling of sample preparation with chromatography techniques were summarized. Typical applications and growing trends of the online coupling techniques were also discussed in detail. With the increasing demands of improving the efficiency, throughput, and analytical capability toward complex samples of the analysis methods, online coupling of sample preparation with chromatography techniques will acquire further development.

show abstract

An automatic versatile system integrating solid-phase extraction with ultra-high performance liquid chromatography–tandem mass spectrometry using a dual-dilution strategy for direct analysis of auxins in plant extracts

Cited by 12 publications

References 41 publications

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

Recent advances of online coupling of sample preparation techniques with ultra high performance liquid chromatography and supercritical fluid chromatography

Contact Info

Product

Resources

About