An Easy-to-Use Polystyrene Microchip-based Cell Culture System

Tazawa, Hidekatsu; Sunaoshi, Shohei; Tokeshi, Manabu; Kitamori, Takehiko; Ohtani-Kaneko, Ritsuko

doi:10.2116/analsci.32.349

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…Many small operation details can affect model properties and experimental reproducibility, and unintended variations may result in widely different results. For example, it is difficult to seed cells at a particular place in a sealed chamber because bubbles in the channel can interfere with the process [101]. In order to improve experimental reproducibility, much more attention will be required to standardize details such as those involved in chip fabrication, cell seeding, cell localization, and other design factors.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Recent Progress in Microfluidic Models of the Blood-Brain Barrier

Jiang

Zheng

et al. 2019

Micromachines

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The blood-brain barrier (BBB) is a critical physical and chemical barrier that maintains brain homeostasis. Researchers in academia and industry are highly motivated to develop experimental models that can accurately mimic the physiological characteristics of the BBB. Microfluidic systems, which manipulate fluids at the micrometer scale, are ideal tools for simulating the BBB microenvironment. In this review, we summarized the progress in the design and evaluation of microfluidic in vitro BBB models, including advances in chip materials, porous membranes, the use of endothelial cells, the importance of shear stress, the detection specific markers to monitor tight junction formation and integrity, measurements of TEER and permeability. We also pointed out several shortcomings of the current microfluidic models. The purpose of this paper is to let the readers understand the characteristics of different types of model design, and select appropriate design parameters according to the research needs, so as to obtain the best experimental results. We believe that the microfluidics BBB models will play an important role in neuroscience and pharmaceutical research.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Recent Progress in Microfluidic Models of the Blood-Brain Barrier

Jiang

Zheng

et al. 2019

Micromachines

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“…Recently, microfluidic cell culture platforms mimicking vascular flow or vascular constriction have been developed [6][7][8][9][10][11][12][13]. A microdevice with a stretchable poly(dimethylsiloxane) (PDMS) membrane as a cell culture substrate, to which cyclic stimulation of circumferential strain was added, has been developed for vascular research [14].…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Cell Stretch Device to Investigate the Effects of Stretching Stress on Artery Smooth Muscle Cell Proliferation in Pulmonary Arterial Hypertension

2018

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A microfluidic cell stretch device was developed to investigate the effects of stretching stress on pulmonary artery smooth muscle cell (PASMC) proliferation in pulmonary arterial hypertension (PAH). The microfluidic device harbors upper cell culture and lower control channels, separated by a stretchable poly(dimethylsiloxane) membrane that acts as a cell culture substrate. The lower channel inlet was connected to a vacuum pump via a digital switch-controlled solenoid valve. For cyclic stretch at heartbeat frequency (80 bpm), the open or close time for each valve was set to 0.38 s. Proliferation of normal PASMCs and those obtained from patients was enhanced by the circumferential stretching stimulation. This is the first report showing patient cells increased in number by stretching stress. These results are consistent with the abnormal proliferation observed in PAH. Circumferential stretch stress was applied to the cells without increasing the pressure inside the microchannel. Our data may suggest that the stretch stress itself promotes cell proliferation in PAH.

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“…11,12 We previously developed immuno-pillar chips as a rapid, inexpensive, easy-touse, and highly sensitive chip applicable for multiplex assays. [13][14][15] Here we report the detection of cat Cys-C (cCys-C) using the immuno-pillar chips.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

et al. 2016

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We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 mu L versus 100 mu L). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL(-1) is comparable to that of the conventional method (1 ng mL(-1)) and it is in the clinically relevant range

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An Easy-to-Use Polystyrene Microchip-based Cell Culture System

Cited by 7 publications

References 21 publications

Recent Progress in Microfluidic Models of the Blood-Brain Barrier

Recent Progress in Microfluidic Models of the Blood-Brain Barrier

A Microfluidic Cell Stretch Device to Investigate the Effects of Stretching Stress on Artery Smooth Muscle Cell Proliferation in Pulmonary Arterial Hypertension

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

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