“…In addition, it has also been reported that a number of metal compounds show their carcinogenic effects due to damaging of DNA in the presence of H 2 O 2 [8][9][10][11]. 0162 HEPES (2-[4-(2-hydroxyethyl)piperazinyl]ethanesulfonic acid) is a popular pH buffer (GoodÕs buffer) that is extensively used in laboratories of analytical, inorganic, physical, biological, biochemical and also in tissue culture [12,13]. This is because of its convenient pK a (7.55), high solubility, and minimal complexation with metal ions [14,15].…”