An Efficient<i>In Vitro</i>Plantlet Regeneration from Shoot Tip Cultures of<i>Curculigo latifolia</i>, a Medicinal Plant

Babaei, Nahid; Abdullah, Nur Ashikin Psyquay; Saleh, Ghizan; Abdullah, Thohirah Lee

doi:10.1155/2014/275028

Cited by 25 publications

(17 citation statements)

References 42 publications

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“…Low concentrations of thidiazuron can stimulate axillary shoot proliferation while higher concentrations can stimulate axillary and adventitious shoot formation. Thidiazuron has also shown promise for micropropagation of some recalcitrant species in the Liliaceae and other plant families [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Issac

Kuriakose

Leung

et al. 2021

Plants

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Xerophyllum asphodeloides (Xerophyllaceae), known as eastern turkeybeard, is an herbaceous perennial found in eastern North America. Due to decline and destruction of its habitat, several states rank X. asphodeloides as “Imperiled” to “Critically Imperiled”. Protocols for seed cryopreservation, in vitro germination, sustainable shoot micropropagation, shoot establishment in soil, and seed germination are presented. Seeds from two tested sources were viable after 20 months of cryopreservation. Germination of isolated embryos in vitro was necessary to overcome strong seed dormancy. Shoot multiplication and elongation occurred on ½ MS medium without PGRs. Shoots rooted in vitro without PGRs or with 0.5 mg/L NAA or after NAA rooting powder treatment and placement in potting mix. When planted in wet, peaty soil mixes, shoots grew for two months and then declined. When planted in a drier planting mix containing aged bark, most plants continued growth. In the field, plant survival was 73% after three growing seasons. Safeguarding this species both ex situ and in situ is possible and offers a successful approach to conservation. Whole seeds germinated after double dormancy was overcome by incubation under warm moist conditions for 12 weeks followed by 12 weeks cold at 4 °C and then warm.

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Section: Discussionmentioning

confidence: 99%

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Issac

Kuriakose

Leung

et al. 2021

Plants

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“…Penggunaan TDZ 1 mg L -1 + NAA 0,1 mg L -1 dan Zeatin 0,1 mg L -1 + NAA 0,01 mg L -1 menghasilkan tinggi tunas lebih rendah dibandingkan dengan perlakuan lainnya. Babaei et al (2014) dalam penelitiannya menyatakan bahwa peningkatan konsentrasi sitokinin dan auksin dapat menurunkan tinggi tunas.…”

Section: H Asil Dan P Em B Ahasanunclassified

Pertumbuhan tunas kunyit tinggi kurkumin pada berbagai jenis sitokinin dan auksin secara in vitro

Suminar¹,

Sobarna²,

Mubarok³

et al. 2021

kultivasi

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Kunyit merupakan salah satu tanaman yang banyak dimanfaatkan oleh masyarakat sebagai bahan baku obat tradisional juga bahan bumbu dapur serta zat pewarna alami sehingga kebutuhannya mengalami peningkatan setiap tahunnya Untuk memenuhi kebutuhan kunyit baik dalam maupun luar negeri diperlukan upaya untuk pengembangan bahan tanam kunyit yang bermutu terutama dalam penyediaan bibit yang baik agar produktivitas tinggi. Metode kultur in vitro dapat digunakan untuk menghasilkan bibit yang bermutu dalam jumlah banyak dalam waktu yang singkat, namun perlu optimasi media yang sesuai. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan konsentrasi sitokinin (9 mg L-1 BAP; 1.0 mg L-1 TDZ; 0.1 mg L-1 Zeatin) dan auksin (0.01 mg L-1 NAA; 1 mg L-1 NAA). Pengamatan dilakukan terhadap perubah persentase eksplan hidup, jumlah tunas, tinggi tunas, dan jumlah daun. Hasil percobaan menunjukkan bahwa kombinasi 9 mg L-1 dan 1 mg L-1 NAA menghasilkan rata-rata jumlah tunas yang lebih tinggi asal eksplan tunas secara in vitro.

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“…So, it is unknown whether C. capitulata is self-compatible or self-incompatible. The owers, roots, stems, and leaves of Curculigo plants have traditionally been used as medicines [10][11][12][13][14][15]. Notably, C. latifolia fruits, but not those of C. capitulata, produce a taste-modifying protein, neoculin, that makes sour-tasting foods or water taste sweet [1,16,17,18].…”

Section: Introductionmentioning

confidence: 99%

De novo transcriptome analysis and comparative expression profiling of genes associated with the taste-modifying protein neoculin in Curculigo latifolia and Curculigo capitulata fruits

Okubo

Terauchi

Okada

et al. 2021

Preprint

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Background Curculigo latifolia is a perennial plant endogenous to Southeast Asia whose fruits contain the taste-modifying protein neoculin, which binds to sweet receptors and makes sour fruits taste sweet. Although similar to snowdrop (Galanthus nivalis) agglutinin (GNA), which contains mannose-binding sites in its sequence and 3D structure, neoculin lacks such sites and has no lectin activity. Whether the fruits of C. latifolia and other Curculigo plants contain neoculin and/or GNA family members was unclear. Results Through de novo RNA-seq assembly of the fruits of C. latifolia and the related C. capitulata and detailed analysis of the expression patterns of neoculin and neoculin-like genes in both species, we assembled 85,697 transcripts from C. latifolia and 76,775 from C. capitulata using Trinity and annotated them using public databases. We identified 70,371 unigenes in C. latifolia and 63,704 in C. capitulata. In total, 38.6% of unigenes from C. latifolia and 42.6% from C. capitulata shared high similarity between the two species. We identified ten neoculin-related transcripts in C. latifolia and 15 in C. capitulata, encoding both the basic and acidic subunits of neoculin in both plants. We aligned these 25 transcripts and generated a phylogenetic tree. Many orthologs in the two species shared high similarity, despite the low number of common genes, suggesting that these genes likely existed before the two species diverged. The relative expression levels of these genes differed considerably between the two species: the transcripts per million (TPM) values of neoculin genes were 60 times higher in C. latifolia than in C. capitulata, whereas those of GNA family members were 15,000 times lower in C. latifolia than in C. capitulata. Conclusions The genetic diversity of neoculin-related genes strongly suggests that neoculin genes underwent duplication during evolution. The marked differences in their expression profiles between C. latifolia and C. capitulata may be due to mutations in regions involved in transcriptional regulation. Comprehensive analysis of the genes expressed in the fruits of these two Curculigo species helped elucidate the origin of neoculin at the molecular level.

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An EfficientIn VitroPlantlet Regeneration from Shoot Tip Cultures ofCurculigo latifolia, a Medicinal Plant

Cited by 25 publications

References 42 publications

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Pertumbuhan tunas kunyit tinggi kurkumin pada berbagai jenis sitokinin dan auksin secara in vitro

De novo transcriptome analysis and comparative expression profiling of genes associated with the taste-modifying protein neoculin in Curculigo latifolia and Curculigo capitulata fruits

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