The decrease of soybean productivity was caused by low quality of seed. To improve the quality of seed, soybean seed were coated with Trichoderma sp. and adding bokashi organic fertilizer. This research aimed at finding the best dose combination of Trichoderma sp. and bokashi fertilizer to improve the quality of soybeans. The research was conducted in the experiment field and Laboratory of Seed Technology at Padjadjaran University in April - August 2017. The experimental design in this research was Randomized Block Design (RBD). The treatments were the combination of four dose values of coating the seed by Trichoderma sp. (0g/100 seeds, 1g/100 seeds, 2g/100 seeds, and 3g/100 seeds) and three dose of bokashi (0g/polybag, 300g/polybag, and 600g/polybag) and each treatment was replicated three times. The experiment result showed that all treatments on soybean seeds did not affect the germination percentange, vigor index, and conductivity value, but affected the seed quantityof 600g/polybag of bokashi and without seed coating with Trichoderma sp. on 100 seed mass and seed weight per plant.
Sari. Trichoderma spp merupakan golongan fungi yang bersifat antipatogen, khususnya layu kecambah, sedangkan bokashi merupakan kompos yang diperkaya dengan beberapa mikroba yang bermanfaat bagi pertumbuhan tanaman.Tulisan ini merupakan rangkuman penelitian pot menggunakan kompos Trichoderma spp sebagai pelapis benih terdeteriorasi disertai bokashi kotoran hewan yang dilakukan pada tanah lahan kering dan tanah sawah pada tahun 2014 dan 2017. Percobaan pertama membandingkan efektivitas beberapa agen hayati sebagai pelapis benih pengaruhnya terhadap vigor benih dan hasil tanaman. Hasil percobaan menunjukkan penggunaan kompos Trichoderma hasilnya lebih baik dibandingkan agen hayati lainnya. Hasil percobaan selanjutnya menunjukkan bahwa pelapisan benih dengan 1 sampai 3 g kompos Trichoderma spp per 100 butir benih pengaruhnya tidak nyata dibandingkan dengan penggunaan pelapis benih berupa pestisida, baik terhadap vigor maupun hasil biji per tanaman. Pemberian bokashi sebaliknya secara nyata mampu meningkatkan hasil biji per tanaman . Pemberian bokashi 15 t ha-1menghasilkan biji seberat 19.83 g per tanaman atau setara dengan 2,379 t ha-1 atau 27,3 % lebih tinggi dari kontrol.Kata kunci : Benih terdeteriorasi, pelapisan benih, Trichoderma, Bokashi Abstract. Trichoderma spp is a group of antipatogenic fungi, especially seedling wilted, while bokashi is a compost enriched with some microbes that are beneficial to plant growth. This paper is a compilation of pot experiment using Trichoderma compost for coating deteriorated seed and accompanied by bokashi on dry land soil and paddy soil in 2014 and 2017 respectively. The first experiment compares the effectiveness of several biological agents as a seed coating of its effect on seed vigor and yield. The results of first experiment showed that the use of 2 g Trichoderma compost 100 seeds-1 was better than other biological agents. However between 1.2 and 3 g of Trichoderma compost per 100 seeds was not significant effect on seed vigor and yield. The results of experiment showed that the coating of seeds with 1 – 3 g of Trichoderma spp compost 100 compared with pesticide seed coatings. Increased of yield only affected bokashi application. Application of bokashi 15 t ha-1 abble to produce 19.83 g seed per plant or equivalent to 2.379 t ha-1 or 27.3% higher than the control.Key words: deteriorated seeds, seed coating, Trichoderma, bokashi
Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture
Kunyit merupakan salah satu tanaman yang banyak dimanfaatkan oleh masyarakat sebagai bahan baku obat tradisional juga bahan bumbu dapur serta zat pewarna alami sehingga kebutuhannya mengalami peningkatan setiap tahunnya Untuk memenuhi kebutuhan kunyit baik dalam maupun luar negeri diperlukan upaya untuk pengembangan bahan tanam kunyit yang bermutu terutama dalam penyediaan bibit yang baik agar produktivitas tinggi. Metode kultur in vitro dapat digunakan untuk menghasilkan bibit yang bermutu dalam jumlah banyak dalam waktu yang singkat, namun perlu optimasi media yang sesuai. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan konsentrasi sitokinin (9 mg L-1 BAP; 1.0 mg L-1 TDZ; 0.1 mg L-1 Zeatin) dan auksin (0.01 mg L-1 NAA; 1 mg L-1 NAA). Pengamatan dilakukan terhadap perubah persentase eksplan hidup, jumlah tunas, tinggi tunas, dan jumlah daun. Hasil percobaan menunjukkan bahwa kombinasi 9 mg L-1 dan 1 mg L-1 NAA menghasilkan rata-rata jumlah tunas yang lebih tinggi asal eksplan tunas secara in vitro.
Peanuts are a potential commodity to be developed in Indonesia after
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