An efficient method for genetic certification of Bacillus subtilis strains, prospective producers of biopreparations

Terletsky, Valeriy P.; Tyshchenko, V. I.; Novikova, I. I.; Boikova, I. V.; Tyulebaev, S. D.; Shakhtamirov, I. Ya.

doi:10.1134/s0026261716010136

Cited by 6 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Precise knowledge of the genetic diversity of microorganisms is useful for multiple purposes, including understanding of the emergence of pathogens, tracking and controlling their spread, as well as certifying and patenting strains of interest for biotechnologies [1][2][3]. DNA "fingerprinting" of bacteria began to develop with the understanding of the role of this molecule and improved throughout the development of DNA technologies.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan

et al. 2021

View full text Add to dashboard Cite

Tularemia is a highly dangerous zoonotic infection due to the bacteria Francisella tularensis. Low genetic diversity promoted the use of polymorphic tandem repeats (MLVA) as first-line assay for genetic description. Whole genome sequencing (WGS) is becoming increasingly accessible, opening the perspective of a time when WGS might become the universal genotyping assay. The main goal of this study was to describe F. tularensis strains circulating in Kazakhstan based on WGS data and develop a MLVA assay compatible with in vitro and in silico analysis. In vitro MLVA genotyping and WGS were performed for the vaccine strain and for 38 strains isolated in Kazakhstan from natural water bodies, ticks, rodents, carnivores, and from one migratory bird, an Isabellina wheatear captured in a rodent burrow. The two genotyping approaches were congruent and allowed to attribute all strains to two F. tularensis holarctica lineages, B.4 and B.12. The seven tandem repeats polymorphic in the investigated strain collection could be typed in a single multiplex PCR assay. Identical MLVA genotypes were produced by in vitro and in silico analysis, demonstrating full compatibility between the two approaches. The strains from Kazakhstan were compared to all publicly available WGS data of worldwide origin by whole genome SNP (wgSNP) analysis. Genotypes differing at a single SNP position were collected within a time interval of more than fifty years, from locations separated from each other by more than one thousand kilometers, supporting a role for migratory birds in the worldwide spread of the bacteria.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan

et al. 2021

View full text Add to dashboard Cite

show abstract

“…В 60-80 % случаев при злокачественном отеке у животных и газовой гангрене у человека выделяют Cl. perfringens типа А в чистом виде или в ассоциации с другими микроорганизмами [8]. Морфология может варьировать, например, на углеводных средах могут образовывать форму коккобактерии, а на белковых средах -нити с заостренными концами длинной до 145 мкм., образуют капсулы (единственный капсулообразующий вид среди патогенных клостридий).…”

Section: Introductionunclassified

Biological Properties of Cl. Perfringens Strains

Toleukhan,

Kirkimbayeva,

Zhylkaidar

2023

jour

View full text Add to dashboard Cite

show abstract

The use of single-nucleotide polymorphism in creating a crossline of meat simmentals

Tyulebaev

Kadysheva

Литовченко

et al. 2019

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The article presents the materials of the study on bull cross of the Bredy meat type simmentals, which include the genotyping of animals for the CAST and CAPN1 genes with the identification of frequencies of genotype and alleles, as well as testing the production of these animals expressed in the evaluation of aged within 8 days of meat for the indicators tenderness, juiciness, organoleptic assessment of taste. The results of the studies did not confirm, with certainty, the influence of the CAST gene polymorphism on the beef tenderness indicators, as well as no associations of this gene with the studied juiciness and meat taste indicators, while the carriers of the homozygous CC genotype CAPN1 exceeded other genotypes of the sample in tenderness, juiciness and taste of beef. And indicators of bull meat with a combination of TT* genes on CAST and CC* on CAPN1 had superiority over the average indicators of the total sample (P <0.001), as well as over the group of animals with the most preferred CC genotype for the CAPN1 gene by 1.22 points or 15.4% (P <0.01) by tenderness, by 1.44 points or 18.2% (P <0.05) by juiciness, by 1.56 points or 19.2% (P <0.001) by taste. Studies have confirmed the effect of the CAPN1 gene CC homozygotes polymorphic state itself, as well as the CAST and CAPN1 genes on beef tenderness in the part where the combination of TT genotypes in the CAST gene and CC in the CAPN1 gene gives a positive effect on beef tenderness, probably associated with activation the activity of μ-calpain in connection with the weakening of the effect of calpastatin as an inhibitor on μ-calpain, as a result of which the enzymatic effect on myofibrillary proteins acquires a different intensity, and possibly a different manifestation. What was the reason for the increase in sensory sensitivity to taste of prepared samples, tenderness and juiciness of meat.

show abstract

An efficient method for genetic certification of Bacillus subtilis strains, prospective producers of biopreparations

Cited by 6 publications

References 11 publications

Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan

Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan

Biological Properties of Cl. Perfringens Strains

The use of single-nucleotide polymorphism in creating a crossline of meat simmentals

Contact Info

Product

Resources

About