An efficient micropropagation protocol for direct organogenesis from nodal explants of medicinal climber, Tylophora indica

Najar, Rafeeq Ahmad; Fayaz, Mufi da; Bhat, Musadiq Hussain; Bashir, Mudasir; Kumar, Amit; Jain, Ashok Kumar

doi:10.21786/bbrc/11.1/20

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…In several cases, due to the presence of endogenous auxin in plants, callus is formed [20]. The current findings on the effects of auxin were also supported by a previous study on Tylophora indica in which a combination of BAP and IBA in as low as 1.23 µM of a basal medium significantly reduced the growth of the plant compared to same cultured in BAP alone [21]. Similarly, another study on Spilanthes acmella Murr.…”

Section: Discussionsupporting

confidence: 86%

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In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

Haida

Nakasha

Hakiman

2020

Plants

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Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia’s herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-cancer, anti-venom and anti-viral activities. Due to its high pharmacological properties, the species has been overexploited to meet the demands of the pharmaceutical industry. The present study was conducted to establish a suitable in vitro culture procedure for the mass propagation of C. nutans. Murashige and Skoog (MS) basal medium, supplemented with different types of cytokinins, auxins, basal medium strength and sucrose concentrations, were tested. Based on the results, a full-strength MS basal medium supplemented with 12 µM 6-benzylaminopurine (BAP) and 30 g/L sucrose was recorded as the best outcome for all the parameters measured including the regeneration percentage, number of shoots, length of shoots, number of leaves and fresh weight of leaves. In the analysis of the phenolics content and antioxidant activities, tissue-cultured leaf extracts assayed at 100 °C exhibited the highest phenolic content and antioxidant activities. The propagation of C. nutans via a plant tissue culture technique was recorded to be able to produce high phenolic contents as well as exhibit high antioxidant activities.

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Section: Discussionsupporting

confidence: 86%

“…reported that the supplementation of cytokinin alone in the basal medium produced better growth [ 22 ]. In both of these studies, the callus formation was observed in the basal regions of the explants [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

Haida

Nakasha

Hakiman

2020

Plants

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“…Several studies reported the success of direct shoot organogenesis from nodal explants. Direct shoot organogenesis of maize was carried out with nodal explants cultured on medium supplemented with 1.8 mg/l benzyladenine ( Mushke, Yarra & Bulle, 2016 ), while compared with three different nodal explants, Tylophora indica showed the highest efficiency of direct shoot organogenesis ( Najar et al, 2018 ). New shoots emerged from the leaf axils of nodal explants, possibly influenced by apical dominance that promoted multiple shoots ( Oliveira et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of pokeweed (Phytolacca americana L.) and comparison of phenolic, flavonoid content, and antioxidant activity between pokeweed callus and other parts

Trunjaruen

Luecha

Taratima

2022

PeerJ

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Background Pokeweed (Phytolacca americana L.) is regarded as an invasive plant in many parts of the world but possesses therapeutic characteristics used for antitumor and rheumatism treatment. This study investigated the effects of auxins and four explants on pokeweed callus induction. The effects of cytokinins and combinations between cytokinins and NAA on shoot and root induction were also studied. TPC, TFC and antioxidant activity of calli were screened and compared with other pokeweed plant parts. Methods Four explants were used to induce callus using 2,4-D and IBA at 1, 2, 3 and 4 mg/l for each auxin. Direct shoot organogenesis from nodal explants was investigated using BAP, kinetin and TDZ (1, 2 and 4 mg/l for each cytokinin). Combined effects between cytokinins and NAA at 0.1, 0.2 and 0.3 mg/l were further simultaneously estimated with root induction. Calli derived from the leaves were compared with other plant parts for TPC, TFC and antioxidant activity using the Folin-Ciocalteu, AlCl3 colorimetric assay and DPPH assays, respectively. Results Results showed that MS medium containing 2 mg/l 2,4-D induced callus formation on leaf explants that provided highest fresh and dry weights. Three types of synthetic cytokinins as kinetin, TDZ and BAP were used for direct shoot organogenesis from pokeweed nodes. MS medium containing 2 mg/l kinetin was effective in stimulating normal shoots, with the largest number of shoots and leaves and the longest shoots. The combination between cytokinins and NAA showed no positive effect on shoot and root induction from pokeweed nodal explants. For TPC and TFC determination, pokeweed seeds and leaves possessed the highest phenolic and flavonoid contents, respectively. Highest phenolic content of pokeweed seeds led to lowest IC50 by DPPH assay. Phenolic content was higher than flavonoid content. Conclusion Results suggested promising conditions for callus induction. Leaf explants cultured on MS medium with 2 mg/l 2,4-D and nodal explants cultured on MS medium with 2 mg/l kinetin provided the largest number of normal shoots and leaves. NAA did not show positive effects on shoot and root induction when combined with cytokinins. Chemical constituent screening indicated that seeds and leaves provided highest TPC and TFC, respectively, while pokeweed calli contained higher phenolic than flavonoid content. This is the first report describing chemical constituent screening and antioxidant activity of calli and other parts of the pokeweed plant. Results provided significant information to further enhance bioactive compound contents of pokeweed calli using elicitation methods.

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“…The effect of culture media's strength on root induction has been studied by Deepa and Thomas [7], Shekhawat et al [40], Slesak et al [41], and Abdelaleem [42] and concluded that half-strength MS is superior in root induction than full strength MS medium. The efficiency of IBA in inducing roots was well documented in other systems such as Tylophora indica [43], Oldenlandia corymbose [44], and Gymnostachyum febrifugum [45]. In other plant species, such as gallnut trees [46], Chinese tallow trees [47], cherry trees [48] and apple trees [49], a low level of macronutrients in the medium was more favorable for in vitro rooting.…”

Section: (A) (C) (B) (D) (F) (E)mentioning

confidence: 98%

Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil

Klaocheed,

Rittirat,

Kalawong

et al. 2024

Trends Sci

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Petiole segments of an economically ornamental plant Oxalis triangularis A.st.-Hil have been used in the present study for rapid, reliable and systematic in vitro propagation. The morphogenic effect of α-Naphthalene acetic acid (NAA) at various concentrations (0.5 - 3.0 mg/L) was studied individually or in combination with 6-benzylaminopurine (BAP). Superior multiplication rates were achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP. Of the tested combinations, maximum shoot regeneration (100.00 %), mean shoot number (14.60 ± 0.37 shoots per segment), and shoot length (18.11 ± 0.30 mm) were recorded on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP after 6 weeks of incubation. Half-strength MS medium augmented with 1.0 mg/L indole-3-butyric acid (IBA) was the optimal medium for in vitro root induction, (24.60 ± 0.37 roots per shoot with 9.10 ± 0.32 mm in length) after 6 weeks of incubation. The regenerated plantlets showed 100 % survival success during acclimatization and exhibited normal growth characteristics and morphology. This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil. HIGHLIGHTS The tissue culture technology was successfully employed for developing a practicable simple, efficient, and amenable protocol for mass multiplication of Oxalis triangularis A.st.-Hil, an economically ornamental plant Our studies based on interaction and combinations of auxins with BAP provided better shoot proliferation The regenerated plantlets survived well under field conditions This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil GRAPHICAL ABSTRACT

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An efficient micropropagation protocol for direct organogenesis from nodal explants of medicinal climber, Tylophora indica

Cited by 7 publications

References 9 publications

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

Micropropagation of pokeweed (Phytolacca americana L.) and comparison of phenolic, flavonoid content, and antioxidant activity between pokeweed callus and other parts

Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil

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