An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran, P.; Jayabalan, N.

doi:10.1079/ivp2005667

Cited by 101 publications

(63 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). Similar results have been reported from studies in Carlina acaulis (Trejgell et al, 2009) and other Asteraceae species (Joshi and Dhar, 2003;Baskaran and Jayabalan, 2005;Trejgell et al, 2010). The presence of auxin was necessary for induction of roots on C. onopordifolia shoots (Tab.…”

Section: Resultssupporting

confidence: 89%

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell¹,

Tretyn²

2011

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

This paper is the first published report describing micropropagation of Carlina onopordifolia, using shoot tip and hypocotyl explants. The explants were excised from 10-day-old seedlings and transferred to proliferation medium supplemented with 6-benzylaminopurine (BA; 1.0 or 3.0 mg l -1 ), kinetin (Kn; 1.0 or 3.0 mg l -1 ) or zeatin (ZEA; 1.0 or 3.0 mg l -1 ) in combination with naphthaleneacetic acid (NAA; 0.1 mg l -1 ). The shoot tips were significantly better than hypocotyls as initial material for shoot regeneration. For shoot multiplication, MS medium supplemented with BA proved superior to the other cytokinins tested. Medium supplemented with 1.0 mg l -1 BA gave the highest shoot propagation frequency (66.9%) and number of shoots per explant (2.5). Single shoots were separated from each other and rooted on MS supplemented with IBA for the whole period of culture, with longor short-pulse IBA application. The highest rooting frequency (84.8%) and root number (18.8) were for shortpulse (1 min) 1000 mg l -1 IBA solution. The higher IBA concentration stimulated callus formation and the development of short roots. The shoots were transferred to MS medium without growth regulators. Survival was highest (54.4%) for the plants from the short-pulse 100 mg l -1 IBA treatment. After 8 weeks of acclimatization the plantlets were removed to field conditions and grew normally.K Ke ey y w wo or rd ds s: : Carlina onopordifolia, micropropagation, cytokinins, rooting, auxin.

show abstract

Section: Resultssupporting

confidence: 89%

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell¹,

Tretyn²

2011

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

show abstract

“…The shoots obtained in the medium with Kn (0.5 -5 mg/l), showed significant increase in leaf size, internode elongation and stem thickness but at the optimum concentration of kinetin (2 mg/l) simultaneous rooting was minimized and the increase in leaf size, internode elongation and stem thickness was also negligible. Kinetin was also found to be more effective than BAP for promoting shoot length as reported earlier (Baskaran and Jayabalan 2005) (Table 2). For shoot proliferation, shoot bud induced from shoot tip and nodal explants on Kn (2 mg/l ) was sectored into clumps (2 -3 shoots each) and subcultured on the medium containing Kn/ BAP in combination with GA3 (0.5 mg/l ) at an interval of 10 -15 days.…”

Section: Resultssupporting

confidence: 79%

Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

Bardar

Kaul

Kachhwaha

et al. 2015

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

This study highlights the effect of different inorganic micronutrients like copper, cobalt, molybdenum, zinc, boron, iodine, iron and manganese in accelerating and amplifying in vitro shoot bud induction and proliferation of a medicinally important plant, Eclipta alba (L.) Hassk. Direct shoot bud induction was observed on MS fortified with Kn (2 mg/l). However, maximum number of shoots was achieved when GA3, 0.5 mg/l was added to induction medium along with 1μM copper sulphate (ten times the normal MS level). Optimization of nutrient level in the basal medium promoted maximum regeneration response from both shoot tips and nodal explants. Elongated shoots were rooted in MS supplemented with IBA, 1.0 mg/l. Healthy, green plantlets with well developed roots, flowered normally in the field. Genetic stability of micropropagated plantlets was evaluated using RAPD markers. The amplification products were monomorphic in micropropagated plantlets and similar to those of mother plant revealing the genetic uniformity of plantlets. The regeneration protocol is highly efficient and reproducible so would be useful for mass multiplication, ex situ conservation and genetic transformation of E. alba (L.) Hassk.

show abstract

“…1). The inhibitory effect of BA on shoot growth has also been noted in reports on regeneration of different Asteraceae species such as Saussurea obvallata (Joshi and Dhar, 2003), Eclipta alba (Baskaran and Jayabalan, 2005), Carlina acaulis (Trejgell et al, 2009) and Cirsium pannonicum (Trejgell et al, 2012).…”

mentioning

confidence: 68%

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Trejgell¹,

Chernetskyy²,

Podlasiak³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L -1 BA and 0.05 mg L -1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L -1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species.K Ke ey y w wo or rd ds s: : Taraxacum pieninicum, endangered species, in vitro culture, multiple shoots, benzylaminopurine, hydroponic culture, fertile plants, conservation.

show abstract

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Cited by 101 publications

References 35 publications

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Contact Info

Product

Resources

About