An efficient temporary immersion system for micropropagation of hybrid hazelnut

Latawa, Jyoti; Shukla, Mukund R.; Saxena, Praveen K.

doi:10.1139/cjb-2015-0111

Cited by 35 publications

(21 citation statements)

References 27 publications

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“…Acclimatization of cherry birch resulted in a 37% survival rate, which is low compared with other woody species such as American elm (90%) (Shukla et al 2012), hazelnut (80%) (Latawa et al 2016), and Betula pendula (95%) (Simola 1985). While the survival rate of micropropagated cherry birch plants was low, the plants that survived were healthy, vigorous, and developed into typical cherry birch plants.…”

Section: Discussionmentioning

confidence: 90%

“…Among the cytokinins tested, BA was the most effective in cherry birch for shoot elongation and multiplication. Similarly, BA was more effective for shoot proliferation in a variety of other woody plants including American elm (Shukla et al 2012), hazelnut (Latawa et al 2016), and Betula grossa (Ide 1987). In contrast, shoots of Betula pendula had greater elongation in the presence of 2-IP (Jamison and Renfroe 1990), whereas cherry birch failed to proliferate in response to this cytokinin.…”

Section: Discussionmentioning

confidence: 99%

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In vitro propagation of cherry birch (Betula lentaL.)

Rathwell¹,

Shukla²,

Jones³

et al. 2016

Can. J. Plant Sci.

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An efficient procedure for the micropropagation and conservation of cherry birch (Betula lenta L.), an endangered species in Canada, is reported. The model utilizes in vitro proliferation of fresh and dormant buds from both greenhouse and mature trees. Various factors were evaluated to optimize the protocol, including plant growth regulators, type and concentration of carbohydrates, and the composition of basal salts. 6-benzylaminopurine was the most effective cytokinin for shoot multiplication, with an optimal concentration of 5 μM. Sucrose was a more effective carbohydrate source than glucose, with the highest multiplication rate occurring at 3% sucrose (w/v). Shoot multiplication was similar on Murashige and Skoog (MS) and Driver and Kuniyuki Walnut (DKW) basal salts, but significantly lower on Woody Plant medium (WPM). Culture media with half-strength DKW basal salts and 20 μM indole-3-butyric acid was found to be the best for rooting (80%). Rooted plantlets were acclimatized to the greenhouse environment with a 37% survival rate. The micropropagation technology developed in the study offers excellent opportunities and tools for rapid replenishment of cherry birch trees in their natural environment, long-term conservation, and provides a platform for further research of this nationally endangered species.Key words: micropropagation, Betula lenta, cherry birch, conservation.Résumé : Les auteurs présentent une méthode efficace pour la micropropagation et la préservation du bouleau flexible (Betula lenta L.), une espèce menacée d'extinction au Canada. Le modèle fait appel à la multiplication in vitro de bourgeons frais et dormants prélevés sur des arbres cultivés en serre et matures. Plusieurs facteurs ont été évalués de manière à optimiser le protocole, notamment les hormones de croissance végétales, la nature et la concentration des hydrates de carbone, ainsi que la composition des sels de base. La 6-benzylaminopurine est la meilleure cytokinine pour multiplier les pousses et sa concentration optimale est de 5 μM. Le sucrose est plus efficace que le glucose comme source d'hydrates de carbone, le taux de multiplication le plus élevé ayant été enregistré à la concentration de 3 % (p/v). Les sels de base Murashige and Skoog (MS) et Driver and Kuniyuki Walnut (DKW) permettent une multiplication similaire des pousses, mais celle-ci est significativement plus faible quand on utilise le milieu de culture Woody Plant (WPM). Le milieu de culture contenant la moitié de la dose recommandée de DKW et 20 μM d'acide indole-3-butyrique est le meilleur pour l'enracinement (80 %). Trente-sept pour cent des plantules enracinées ont survécu en serre après acclimatation. La technique de la micropropagation élaborée dans le cadre de cette étude présente d'excellentes possibilités et offre des outils intéressants pour rétablir rapidement la population de bouleaux flexibles dans leur milieu naturel, pour la conservation à long terme et pour des recherches plus poussées sur cette espèce en voie de disparition au pays. [Tra...

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

In vitro propagation of cherry birch (Betula lentaL.)

Rathwell¹,

Shukla²,

Jones³

et al. 2016

Can. J. Plant Sci.

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“…2 was developed to be compatible with both semi-solid culture and liquid based rocker systems [24–28], as well as being a suitable size to integrate commercially available RGB LED strips. One of the major limitations of FDM 3D printing with respect to plant tissue culture is that most materials currently used have relatively low melting points and are not suited to heat sterilization or autoclaving.…”

Section: Resultsmentioning

confidence: 99%

Application of 3D printing to prototype and develop novel plant tissue culture systems

et al. 2017

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BackgroundDue to the complex process of designing and manufacturing new plant tissue culture vessels through conventional means there have been limited efforts to innovate improved designs. Further, development and availability of low cost, energy efficient LEDs of various spectra has made it a promising light source for plant growth in controlled environments. However, direct replacement of conventional lighting sources with LEDs does not address problems with uniformity, spectral control, or the challenges in conducting statistically valid experiments to assess the effects of light. Prototyping using 3D printing and LED based light sources could help overcome these limitations and lead to improved culture systems.ResultsA modular culture vessel design in which the fluence rate and spectrum of light are independently controlled was designed, prototyped using 3D printing, and evaluated for plant growth. This design is compatible with semi-solid and liquid based culture systems. Observations on morphology, chlorophyll content, and chlorophyll fluorescence based stress parameters from in vitro plants cultured under different light spectra with similar overall fluence rate indicated different responses in Nicotiana tabacum and Artemisia annua plantlets. This experiment validates the utility of 3D printing to design and test functional vessels and demonstrated that optimal light spectra for in vitro plant growth is species-specific.Conclusions3D printing was successfully used to prototype novel culture vessels with independently controlled variable fluence rate/spectra LED lighting. This system addresses several limitations associated with current lighting systems, providing more uniform lighting and allowing proper replication/randomization for experimental plant biology while increasing energy efficiency. A complete procedure including the design and prototyping of a culture vessel using 3D printing, commercial scale injection molding of the prototype, and conducting a properly replicated experiment are discussed. This open source design has the scope for further improvement and adaptation and demonstrates the power of 3D printing to improve the design of culture systems.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-017-0156-8) contains supplementary material, which is available to authorized users.

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“…By this approach, as outlined before, the liquid medium does not reach the explants forced by air entering the flask but by mechanical movement of the bioreactor (Figure 4). Hazelnut and cedar shoots proliferated more by TIS than by SS, showing higher content of photosynthetic pigments [41], as well as larger leaves and more vigorous shoots [41,42]. Although hazelnut shoots rooted better when adventitious rooting was induced in SS than by TIS without ventilation [41], the rocker system did enhance root formation and acclimation (up to 98%) in the case of Cedrela odorata [42].…”

Section: Axillary Shoots Cultured By Temporary Immersionmentioning

confidence: 93%

Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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An efficient temporary immersion system for micropropagation of hybrid hazelnut

Cited by 35 publications

References 27 publications

In vitro propagation of cherry birch (Betula lentaL.)

In vitro propagation of cherry birch (Betula lentaL.)

Application of 3D printing to prototype and develop novel plant tissue culture systems

Use of bioreactor systems in the propagation of forest trees

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