An electrochemical impedance model for integrated bacterial biofilms

Ben‐Yoav, Hadar; Freeman, Amihay; Sternheim, Marek; Shacham‐Diamand, Yosi

doi:10.1016/j.electacta.2010.12.025

Cited by 55 publications

(35 citation statements)

References 39 publications

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“…One of the emerging methods for biofilm detection is the use of electrochemical impedance spectroscopy (EIS) measures by employing polymeric sensors. [12][13][14][15][16][17] In EIS, 4 the electrochemical impedance across the electrode-electrolyte interface is measured over a wide range of frequencies to elicit information about the properties of the interface. In the case of biofilms, successful detection is based on changes linked to charge transfer resistance and capacitance corresponding to the maturing stages of biofilm development.…”

Section: Introductionmentioning

confidence: 99%

Predicting Adsorption Energies Using Multifidelity Data

Tian

Rangarajan

2019

J. Chem. Theory Comput.

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Composite i-PP/PEDOT films made of isotactic polypropylene (i-PP), which is frequently used for the fabrication of implantable medical devices for internal use, and chemically synthesized poly(3,4-ethylendioxythiophene) (PEDOT) nanoparticles, which are electroactive and biocompatible, have been prepared and used to detect biofilm infection. After chemical and morphological characterization, the properties (interfacial, mechanical, thermal and electrochemical) and biocompatibility of i-PP/PEDOT have been examined. Besides, carbon screen-printed electrodes coated with i-PP/PEDOT have been found to detect the growth of Gram-positive and Gram-negative bacteria through the oxidation of nicotinamide adenine dinucleotide (NADH), which comes from the bacteria metabolism (i.e. the respiration). Thus, as outer bacterial membranes are permeable to cytosolic NADH, this metabolite has been found to be an appropriated target for the detection of growing bacterial infections (biofilms). In addition, the sensor does not respond towards eukaryotic cells. This is because the major NADH pool in eukaryotic cells is located at the mitochondria and, therefore, the concentration of in the medium is not high enough to be detected since the inner mitochondrial membrane is impermeable to NADH or NAD+.

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Section: Introductionmentioning

confidence: 99%

Predicting Adsorption Energies Using Multifidelity Data

Tian

Rangarajan

2019

J. Chem. Theory Comput.

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“…The schematic bio-hybrid electrode architecture in Figure 5a) shows one possible impedance model circuit for our protein-semiconductor assembly (following a suggestion of Ben-Yoav et al [22]), along with an energy diagram for the solid state processes and the processes in the electrolyte. The water splitting reaction is based on electron holes h + which come from the hematite photoanode: 2 H 2 O +4 h + → O 2 ↑ +4H + .…”

Section: Charge Transfer In Pc-melanin Coated Hematite Filmsmentioning

confidence: 99%

Charge transfer between photosynthetic proteins and hematite in bio-hybrid photoelectrodes for solar water splitting cells

et al. 2015

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Functionalization of the hematite photoanode with the photosynthetic light antenna protein C-phycocyanin (PC) can yield substantial enhancement of the photocurrent density. Photoelectrochemical cells with bio-hybrid electrodes from photosynthetic proteins and inorganic semiconductors have thus potential for the use in artificial photosynthesis. We investigate here processing routes for the functionalization of hematite photoanodes with PC, including in situ co-polymerization of PC with enzymatically-produced melanin, and using a recombinant PC genetically engineered to carry a hexa-histidine tag (αHisPC). First, the effect of the immobilisation of PC on the electrode morphology and photocurrent production is evaluated. Then, the electronic charge transfer in dark and light conditions is assessed with electrochemical impedance spectroscopy and valence band (VB) X-ray photoemission spectroscopy. The relative shift of the VB spectrum towards the Fermi energy E F upon illumination is smaller for the more complex processed coating and virtually disappears for αHisPC immobilised with a melanin film. Optimal conditions for protein immobilisation are determined and the dark currents benefit most from the most advanced protein coating processes.

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“…Basing on the literature [9,14,28,29,32], the measured impedance spectra and knowledge about the physiochemical properties of biofilm [5,6,8,16], the electrical equivalent circuits (EEC) were obtained and are shown in Fig. 5.…”

Section: Electrical Equivalent Circuitsmentioning

confidence: 99%

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

Chabowski¹,

Junka²,

Piasecki³

et al. 2017

Metrology and Measurement Systems

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The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor's surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.

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An electrochemical impedance model for integrated bacterial biofilms

Cited by 55 publications

References 39 publications

Predicting Adsorption Energies Using Multifidelity Data

Predicting Adsorption Energies Using Multifidelity Data

Charge transfer between photosynthetic proteins and hematite in bio-hybrid photoelectrodes for solar water splitting cells

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

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