An enzyme-linked immunosorbent assay (ELISA) for prostate-specific antigen

Stowell, L. I.; Sharman, Lionel E.; Hamel, Keith M.

doi:10.1016/0379-0738(91)90141-5

Cited by 54 publications

(30 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13,14 The increase in incidence of prostate cancer over a period of the last 10 y was related to the increased use of the prostate-specific antigen (TPSA and FPSA) test in diagnosing and screening for prostate cancer risk patients. [12][13][14][15][16][17][18][19] The TPSA is a serine protease produced by the epithelial cells of normal, hyperplastic, and cancerous prostatic tissues. 20 As the enzyme is not specific for prostate cancer, the TPSA test has a high false-positive rate when used as a screening tool.…”

Section: Introductionmentioning

confidence: 99%

Age-specific reference ranges for prostate-specific antigen (PSA) in Jordanian patients

Battikhi

2003

Prostate Cancer Prostatic Dis

View full text Add to dashboard Cite

In this study, the normal distribution of total prostate-specific antigen (TPSA) and free prostate-specific antigen (FPSA) values and the appropriate reference ranges for prostate-specific antigen (PSA) in Jordanian patients were established; the values were then compared to those of other studies. Serum TPSA and FPSA levels in 1852 men with no diagnostic prostate cancer, as well as in patients whose PSA value was obtained as part of the clinical work-up of symptoms relating to nonneoplastic urologic conditions, were estimated during the period 1993-2001: 1561 (84.3%) were above 40 y of age or older. We studied the data as a function of age to determine the usefulness of measuring TPSA, FPSA, and their ratio as screening tests for prostate cancer risk patients. Using the 95th percentile, the recommended age-specific reference ranges of TPSA and FPSA values were as follows: for the age group 30-34 y, 2.3 and 0.51 ng/ml, respectively; for the age group 35-39 y, 2.9 and 0.59 ng/ml, respectively; for the age group 40-44 y, 3.2 and 0.63 ng/ml, respectively; for the age group 45-49 y, 3.75 and 0.71 ng/ml, respectively; for the age group 50-54 y, 3.8 and 0.83 ng/ml, respectively; for the age group 55-59 y 3.75 and 0.96 ng/ ml, respectively; for the age group 460 y old, 4.3 and 1.26 ng/ml, respectively. There was a continuous increase in TPSA and FPSA means and medians throughout the study period with a significant correlation (Po0.001, Po0.05) for TPSA and FPSA levels), respectively, and advancing age group. With regard to the ratios of FPSAto-TPSA for each age group we found no correlation between them. As a result, the appropriate upper limit of normal 95th percentile for all ratios was 0.23 for men for all ages. The establishment of appropriate reference ranges for TPSA and FPSA as well as ratios will allow the practicing urologist to incorporate these new parameters into diagnostic evaluation of men at risk for early, potentially curable prostate cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Age-specific reference ranges for prostate-specific antigen (PSA) in Jordanian patients

Battikhi

2003

Prostate Cancer Prostatic Dis

View full text Add to dashboard Cite

show abstract

“…Increase in %fPSA is associated with BPH while an increase in the cPSA is associated with Prostate cancer [12]. Plasma creatinine ≥ 133 umol/l (1.5 mgldl) defined renal impairment [13].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Serum Prostate Specific Antigen, Some Renal Indices and Uric Acid Levels in Subjects with Benign Prostatic Hyperplasia at Lokoja, Nigeria

Emeje¹,

Ukibe²,

Onyenekwe³

et al. 2017

J Bioanal Biomed

View full text Add to dashboard Cite

between signs and symptoms of BPH and RI in their population-based sample of 467 white men [9]: there was a significant association between RI and BPH. Studies in Africa have shown that countries like Ghana, Nigeria, and Cameroon have similar prevalence of 25% of Benign Prostatic Hyperplasia in kidney disease [10]. In the United State of America, 13.6% of men with BPH have been reported to have chronic renal failure [11]. The impact of BPH and renal impairment on health should not be underestimated as it accounts for about 10% of hospital admission in Nigeria [6]. The most successful approach to detect BPH is the use of percent fPSA= fPSA × 100/tPSA, where f=free and t=total AbstractBackground: Prostate disorders (prostatitis, BPH and Pca) can contribute to renal impairment. Benign prostatic hyperplasia (BPH) and renal impairment (RI) such as chronic kidney disease are important public health problems in older men. The present study aimed to assess serum levels of prostate specific antigen, urea, creatinine, protein and uric acids in subjects with BPH at Federal Medical Center, Lokoja, Kogi State, Nigeria. A population-based sample of one hundred and ten (110) men aged (51-70) years were conveniently recruited and divided into three groups designation A= BPH with RI, (n=35) B=BPH without RI, (n=35) and C=Control, (n=40).Methods: Blood samples were collected from all the participants and serum separated and stored at -20˚C until analyzed for prostate specific antigen using Enzyme Linked Immunosorbent Assay (ELISA) and colorimetric assay method for creatinine, urea, protein and uric acid. Data were analyzed using SPSS software application (version 17.0). Pearson correlation and Receiving Operating Characteristics of the groups were done. Results:The result showed that urea and creatinine levels were significantly higher in BPH subjects with or without renal impairment when compared with controls (p<0.05 respectively). Similarly, total prostate specific antigen (tPSA), free prostate specific antigen (fPSA), complex prostate specific antigen (cPSA) and percent free prostate specific antigen (%fPSA) were significantly higher in BPH subjects with or without RI when compared with controls (p<0.05 respectively). Urea, creatinine and uric acid were significantly higher while total protein was significantly lower in BPH with RI when compared with BPH without RI (p<0.05 respectively). Conclusion:The significantly higher urea, creatinine and uric acid levels in BPH subjects showed that BPH subjects with RI may have decrease excretion and accumulation of uric acid by the kidney suggesting possible risk of progression to CKD while BPH subjects without RI tends to be more prone to developing renal dysfunction. The significant correlation between %fPSA, creatinine and urea shows an association between BPH and renal diseases. Using receiving operating characteristic (ROC) curves to assess diagnostic performance of various parameters in various groups for the prediction of BPH with or without renal disease, there was evidence tha...

show abstract

“…Serum sarcosine was measured by the Colourimetric method 9 ( BioVision Research Products, 980 Linda Vista Avenue, Mountain View, CA 94043 USA. ).Total and free Prostate Specific Antigen (tPSA,fPSA) was estimated by Elisa method 10 while the remaining PSA derivatives were calculated mathematically.The data were analysed statistically for the diagnostic performance of the parameters in PCa patients using Anova, T-test and ROC curves in SPSS package version 17…”

Section: Methodsmentioning

confidence: 99%

Serum Sarcosine and PSA Levels in Patients With Prostate Cancer in Sokoto, Nigeria.

Nnamah¹,

Anaja²,

Mungadi³

et al. 2016

IOSR

View full text Add to dashboard Cite

show abstract

An enzyme-linked immunosorbent assay (ELISA) for prostate-specific antigen

Cited by 54 publications

References 12 publications

Age-specific reference ranges for prostate-specific antigen (PSA) in Jordanian patients

Age-specific reference ranges for prostate-specific antigen (PSA) in Jordanian patients

Assessment of Serum Prostate Specific Antigen, Some Renal Indices and Uric Acid Levels in Subjects with Benign Prostatic Hyperplasia at Lokoja, Nigeria

Serum Sarcosine and PSA Levels in Patients With Prostate Cancer in Sokoto, Nigeria.

Contact Info

Product

Resources

About