An enzyme-linked oligonucleotide assay

Drolet, Daniel; Moon-McDermott, Lotus; Romig, Timothy S

doi:10.1038/nbt0896-1021

Cited by 317 publications

(207 citation statements)

References 42 publications

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“…Drolet et al 26 reported the first enzyme-linked aptamer assay (ELAA) consisting of a mixed ELISA/ELAA sandwich to detect human vascular endothelial growth factor on a microtiter plate. Since then, similar assays have been used with either labeled or immobilized nucleic acid molecules as capturing or detecting agents.…”

Section: Discussionmentioning

confidence: 99%

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Ramos¹,

Piñeiro²,

Soto

et al. 2007

Laboratory Investigation

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Section: Discussionmentioning

confidence: 99%

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Ramos¹,

Piñeiro²,

Soto

et al. 2007

Laboratory Investigation

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“…[60][61][62] Target binding to immobilized aptamers may also increase the mass or refractive index nearby the surface ( Figure 3A). [63][64][65] However, simple binding based assays require extensive washing.…”

Section: Biosensor Design Strategiesmentioning

confidence: 99%

Aptamer-based biosensors for biomedical diagnostics

Zhou

Huang

Ding

et al. 2014

Analyst

475

290

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Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any molecule of choice, can be readily modified at arbitrary positions and they possess predictable secondary structures, this platform technology shows great promise in biosensor development. Over the past two decades, more than one thousand papers have been published on aptamer-based biosensors. Given this progress, the application of aptamer technology in biomedical diagnosis is still in a quite preliminary stage. Most previous work involves only a few model aptamers to demonstrate the sensing concept with limited biomedical impact. This Critical Review aims to summarize progresses that might enable practical applications of aptamer for biological samples. First, general sensing strategies based on the unique properties of aptamers are summarized. Each strategy can be coupled to various signaling methods. Among these, a few detection methods including fluorescence lifetime, flow cytometry, upconverting nanoparticles, nanoflare technology, magnetic resonance imaging, electronic aptamer-based sensors, and lateral flow devices have been discussed in more detail since they are more likely to work in a complex sample matrix. The current limitations of this field include the lack of high quality aptamers for clinically important targets. In addition, the aptamer technology has to be extensively tested in a clinical sample matrix to establish reliability and accuracy. Future directions are also speculated to overcome these challenges.3

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“…DNA15-coated beads were used to detect α-thrombin in human serum. The detection method (a sandwich immunoassay) was analogous to that described by Drolet et al 26 for the enzyme-linked immunosorbent assay (ELISA)-based detection of vascular endothelial growth factor (VEGF) in a serum sample. The thrombin assay was both specific and sensitive, allowing detection of α-thrombin in a serum dilution as low as 0.125%.…”

Section: Discussionmentioning

confidence: 99%

Using Aptamers as Capture Reagents in Bead-Based Assay Systems for Diagnostics and Hit Identification

Porschewski¹,

Grättinger²,

Klenzke³

et al. 2006

SLAS Discovery

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Most applications of xMAP™ (Luminex ® ) bead-based assay technology in diagnostics and drug discovery use immobilized antigens or antibodies. Here the authors describe the development of novel assay systems in which synthetic oligonucleotides that specifically bind and inhibit other biomolecules-so-called aptamers-are directly immobilized on beads. The robustness, specificity, and sensitivity of aptamer-based assays were demonstrated in a test system that detected human α-thrombin in serum samples. xMAP technology was also adapted to competitive screening formats where an aptamer/protein complex was disrupted by a functionally analogous competitor. The results indicate that such assays are excellently suited for diagnostic applications or drug screening, where aptamers serve as competitive binding probes for the identification of small-molecule hits. These methods should be transferable to a large number of applications because specific aptamers can be rapidly generated for almost any protein target. (Journal of Biomolecular Screening 2006:773-781)

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An enzyme-linked oligonucleotide assay

Cited by 317 publications

References 42 publications

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Aptamer-based biosensors for biomedical diagnostics

Using Aptamers as Capture Reagents in Bead-Based Assay Systems for Diagnostics and Hit Identification

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