An evaluation of an InPouch TV culture method for diagnosing Trichomonas vaginalis infection.

Borchardt, Kenneth A.; Smith, Rodney F.

doi:10.1136/sti.67.2.149

Cited by 39 publications

(36 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Even when broth cultures are mixed with antibiotics to decrease contamination by normal flora of the swabbing sites, the major problem in cultivation of trichomonads is control of the contamination by the bacteria. Cultivation of trichomonads are the most effective method to making right epidemiology of trichomonads species, but the culture wasn't easily available in many center, to resolve these issue, they developed the system like In-pouch TV culture (BioMed Diagnostics, USA) where they can inoculated the sample in a two chambered bag, that allow the examiner to use the pouches as wet mount preparation and immediate observation of trichomonads through the pouch bags that use as slide after inoculation with the sample, or can incubation the inoculated pouches to diagnosis the trichomonads by cultivation (Borchardt and Smith., 1991).…”

Section: Resultsmentioning

confidence: 99%

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Hamad¹,

Hassan²

2017

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

Article InfoThis research was conducted to study the spread of an important unicellular protozoan Trichomonas gallinae isolated from the mouth and esophagus of three groups of domestic pigeon Colomba livia domestica by wet mount preparation. A total of 609 domestic pigeons were tested in Kirkuk city, Iraq from August 2015 to April 2016, the total infectious rate of the prevalence were 49.26%, distributed to 64.92%, 50.98% and 30.41% in Alzajel, pakistani and zubaeri pigeon respectively. The present study compared three different stains to characterize the shape of T. gallinae namely Giemz, Gram and for first time Field's stain, the Giemza stain showed efficient to stain T. gallinae more than other two stains, although Field's stain requires less time for staining compared to Giemza and Gram's stain. The parasites are cultivated in three prepared culture media (In-tube culture system), which are modified Diamond media (TYM), Cysteine Peptone Maltose media (CPLM) and Trichomonas CM0161 media (CM 161) with the initial inoculation of 2X105 cell/ml, the fourth media was In-pouch culture system that is commercially available with the initial inoculation of 105 cell/ml. In-pouch TV culture media is used for the first time to diagnose and to grow T. gallinae which is a special media for diagnosis of the vaginal trichomoniasis caused by Trichomonas vaginalis. The result showed the best growth of T. gallinae in In-pouch media compared with In-tube culture system, modified diamond media and CM 161 gave the high growth to 48 hours while CPLM gave the high growth from 24 hours, the CM 161 media showed acute decrease in the number of T. gallinae after 48 hour.

show abstract

Section: Resultsmentioning

confidence: 99%

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Hamad¹,

Hassan²

2017

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

show abstract

“…Reported symptoms of urethral discharge and dysuria were recorded, as well as the number of polymorphonuclear leukocytes on a urethral Gram stain. Two urethral swabs, one for PCR and one for on-site inoculation, were collected into TVInPouch culture medium (BioMed Diagnostics, San Jose, Calif.) (1). A first-fraction voided urine specimen was collected and processed on-site.…”

Section: Methodsmentioning

confidence: 99%

Improved Detection by DNA Amplification of Trichomonas vaginalis in Males

Schwebke

Lawing

2002

J Clin Microbiol

View full text Add to dashboard Cite

Trichomoniasis is a sexually transmitted infection that is highly prevalent worldwide and has been linked to preterm birth and human immunodeficiency virus acquisition. In females, trichomoniasis causes vaginitis, while in males, it is frequently asymptomatic but can be a cause of urethritis. Control efforts have been hampered by the lack of a sensitive diagnostic technique for this infection in males. Men attending a sexually transmitted disease (STD) clinic for a new complaint were screened for Trichomonas vaginalis by culture and by PCR analysis of urine and urethral-swab specimens. The prevalence of Trichomonas determined by culture was 5% (15 of 300 specimens), compared to 17% (52 of 300) determined by PCR. Urine specimens yielded a greater number of positive results by PCR than did urethral-swab specimens. The sensitivity of PCR analysis of urine specimens in comparison to that of culture was 100%. The use of PCR techniques in urine specimenbased detection of T. vaginalis was highly sensitive and revealed a prevalence of infection more than three times that revealed by culture for men at high risk for STDs.Bacterial sexually transmitted diseases (STDs), such as syphilis, gonorrhea, and chlamydia, are declining in the United States; however, infections caused by Trichomonas vaginalis have not evidenced similar declines. Despite the fact that vaginal trichomoniasis has been linked to preterm birth and the acquisition of human immunodeficiency virus (HIV) (3, 11), increased screening efforts have not been made. For women, the most commonly used diagnostic test for Trichomonas is a direct microscopic examination of the vaginal fluid. Although it is highly specific, the sensitivity of this technique in comparison to that of culture ranges from 50 to 80% (9). Culture of vaginal specimens for the organism is the current "gold standard"; however, the use of PCR techniques for detection in females has been studied, with varied results, and current techniques do not appear to have greater sensitivity than culture does (4,12,13,15). Diagnostic techniques for Trichomonas in males are not routinely used, as the current gold standard (culture of urine and urethral swabs) (10) is cumbersome and likely suboptimal. Few studies have addressed the utility of PCR testing for Trichomonas in males. We compared PCR results for urethral and urine specimens to those of culture techniques for the diagnosis of Trichomonas in men attending an STD clinic. MATERIALS AND METHODSHeterosexual males attending the Jefferson County Health Department STD Clinic in Birmingham, Ala., for STD screening or the treatment of its symptoms, were invited to participate in this diagnostic study. The study was approved by the Institutional Review Boards of the Jefferson County Health Department and the University of Alabama at Birmingham. Men were excluded from participation if they had urinated in the previous hour or had taken antibiotics during the preceding 14 days. Reported symptoms of urethral discharge and dysuria were recorded, as well as the nu...

show abstract

“…Different staining techniques, including those using Gram stain, Giemsa stain, Papanicolaou (Pap) smear, and acridine orange (1,26), and diverse molecularly based diagnostic methods, such as hybridization assay and PCR, have been employed to detect T. vaginalis but vary widely in their sensitivities and specificities (5,15,(17)(18)(19)21). Thus, broth culture is still considered the "gold standard" for diagnosis of trichomoniasis (1,24). For this purpose, various liquid culture media have been developed (1,2,10,24,25).…”

mentioning

confidence: 99%

“…Thus, broth culture is still considered the "gold standard" for diagnosis of trichomoniasis (1,24). For this purpose, various liquid culture media have been developed (1,2,10,24,25). However, identification of positive samples requires frequent microscopic observations for up to 7 days.…”

mentioning

confidence: 99%

Detection of Trichomonas vaginalis on Modified Columbia Agar in the Routine Laboratory

2002

View full text Add to dashboard Cite

An evaluation of an InPouch TV culture method for diagnosing Trichomonas vaginalis infection.

Cited by 39 publications

References 7 publications

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Improved Detection by DNA Amplification of Trichomonas vaginalis in Males

Detection of Trichomonas vaginalis on Modified Columbia Agar in the Routine Laboratory

Contact Info

Product

Resources

About