An Evaluation of Different Coating for TiN Microelectrode Chambers Used for Neonatal Cardiomyocytes

Svoboda, O.; Skopalík, Josef; Baiazitova, Larisa; Gabrielová, Eva; Čmiel, Vratislav; Provazník, Ivo; Fohlerová, Zdenka; Hubálek, Jaromír

doi:10.22489/cinc.2016.107-309

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…The SNR for the platform was reported at 11.6 ± 0.5, which the authors reported as standard. Additional examples of ITO metallization for MEAs have been described in literature [99][100][101][102]. [129], with the inset depicting an unprocessed PET layer atop the PCB.…”

Section: Mea Substrates Conductors and Insulation Materialsmentioning

confidence: 99%

Development of in vitro 2D and 3D microelectrode arrays and their role in advancing biomedical research

Didier

Kundu

DeRoo

et al. 2020

J. Micromech. Microeng.

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The development of microelectrode arrays (MEAs) along with complementary advances in electronics, mechanics and software to connect with these arrays has led to the in vitro interfacing and benchtop electrophysiological models of several electrically active cells such as neurons and cardiomyocytes proving vital models and testing of human disease conditions in a dish/on a chip. This topical review deals with the micro/nanofabrication technology development of Microelectrodes Arrays from early silicon based developments to today’s additive manufacturing technologies that have been employed to address bio-micro-electro-mechanical systems tool development in this space. Specifically 2D and 3D MEAs technologies have been reviewed in this paper along with a broad overview of some of the biological applications using these devices that are advancing the very state of biomedical research.

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Section: Mea Substrates Conductors and Insulation Materialsmentioning

confidence: 99%

Development of in vitro 2D and 3D microelectrode arrays and their role in advancing biomedical research

Didier

Kundu

DeRoo

et al. 2020

J. Micromech. Microeng.

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“…The one hundred and twenty electrode 120MEA100/30iR-ITO-gr microelectrode chambers (Multi Channel Systems MCS GmbH) with 12×12 electrode layout ( Figure 1) were coated at 37 °C with 5 µg/ml (Sigma-Aldrich) fibronectin dissolved in 0.02% gelatin from bovine skin (Sigma-Aldrich) at least one hour before cell seeding. The MEA chambers were cleaned and sterilized before use as described earlier [4].…”

Section: Microelectrode Chambersmentioning

confidence: 99%

“…There are some disadvantages of MEA systems, such as complicated preparation of MEA chambers or difficult interpretation of results because of measuring the second signal derivation. The crucial step in MEA experiments is choosing the design of substrate-integrated microelectrodes and coating procedure depending on cell type [4].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Electrical and Fluorescence Recording of HL-1 Cells Electrical Activity in Response to Extracellular Calcium Stimulation

Svoboda

Baiazitova

Čmiel

et al. 2018

2018 Computing in Cardiology Conference (CinC)

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In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model. HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses. Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium (-501±14 µV, 2.1±1.0 Hz). The ASAP1's fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.

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Electrical Stimulation of ASAP1 with a Combination of Microelectrode Array and Fluorescent Techniques

Svoboda

Čmiel

Baiazitova

et al. 2018

2018 41st International Conference on Telecommunications and Signal Processing (TSP)

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An Evaluation of Different Coating for TiN Microelectrode Chambers Used for Neonatal Cardiomyocytes

Abstract: Abstract

Cited by 3 publications

References 8 publications

Development of in vitro 2D and 3D microelectrode arrays and their role in advancing biomedical research

Development of in vitro 2D and 3D microelectrode arrays and their role in advancing biomedical research

Simultaneous Electrical and Fluorescence Recording of HL-1 Cells Electrical Activity in Response to Extracellular Calcium Stimulation

Electrical Stimulation of ASAP1 with a Combination of Microelectrode Array and Fluorescent Techniques

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