An evaluation of the effects of probiotics on tumoral necrosis factor (TNF-α) signaling and gene expression

Vincenzi, Angélica; Goettert, Márcia Inês; Souza, Cláucia Fernanda Volken de

doi:10.1016/j.cytogfr.2020.10.004

Cited by 46 publications

(25 citation statements)

References 85 publications

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“…damage. These findings are in agreement with conclusion reported by Liu et al [53]; Kanmani et al [54]; Vincenzi et al [55] about the action of probiotics in suppress TNF-α release. On the other hand, the three strains and their mixture showed significant increase in the IL-10, the best key anti-inflammatory cytokine in the immune response [56] as they released IL-10 as 281.5-229.5 pg/mg compared to the ulcerative group which showed low level of IL-10 as 121 ±4.0 pg/mg.…”

Section: Plos Onesupporting

confidence: 93%

Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota

et al. 2021

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Our aim was to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9. 150 human fecal samples were collected and used for isolation of vitamin producers—probiotics. 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes. As a result, three isolates were selected and their production of the B2 and B9 were confirmed by HPLC. The three isolates were identified on species level by sequencing their 16S rRNA gene which showed 100% identical to strains of Pediococcus acidilactici. Thus, they were named as P. acidilactici WNYM01, P. acidilactici WNYM02, P. acidilactici WNYM03 and submitted to the Genbank database with accession numbers. They met the probiotic criteria by expressing 90–95% survival rate at pH (2.0–9.0) and bile salt up to 2% for 3 h in addition to their antimicrobial activity against gram positive and negative microorganisms. They also showed no hemolytic activity and common pattern for antibiotic susceptibility. Our three strains were tested individually or in mixture in vivo on rat colitis model compared to ulcerative group. The strains were administrated orally to rats in daily dose containing CFU 109 for 14 days then followed by induction of colitis using acetic acid then the oral administration was continued for more four days. The histology results, the anti-inflammatory and anti-oxidative stress biomarkers showed the protective role of the strains compared to the ulcerative group. As a conclusion, we introduce novel three probiotic candidates for pharmaceutical preparations and health applications.

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Section: Plos Onesupporting

confidence: 93%

Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota

et al. 2021

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“…LPTC were activated with anti-CD3 and anti-CD28 antibodies, and we found that cell proliferation, cytokine secretion and NF-κB pathway activation were suppressed upon exposure to L. kefiri. Collectively, different strains of Lactobacillus and Lactococcus have shown to modulate the inflammatory activity through multiple mechanisms, but especially by inhibiting the translocation of the nuclear transcription factor NF-κB (Vincenzi et al,. 2020;Zeng et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD

et al. 2021

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Ulcerative colitis and Crohn’s disease, the two main forms of inflammatory bowel disease (IBD), are immunologically mediated disorders. Several therapies are focused on activated T cells as key targets. Although Lactobacillus kefiri has shown anti-inflammatory effects in animal models, few studies were done using human mucosal T cells. The aim of this work was to investigate the immunomodulatory effects of this bacterium on intestinal T cells from patients with active IBD. Mucosal biopsies and surgical samples from IBD adult patients (n = 19) or healthy donors (HC; n = 5) were used. Lamina propria mononuclear cells were isolated by enzymatic tissue digestion, and entero-adhesive Escherichia coli-specific lamina propria T cells (LPTC) were expanded. The immunomodulatory properties of L. kefiri CIDCA 8348 strain were evaluated on biopsies and on anti-CD3/CD28-activated LPTC. Secreted cytokines were quantified by ELISA, and cell proliferation and viability were assessed by flow cytometry. We found that L. kefiri reduced spontaneous release of IL-6 and IL-8 from inflamed biopsies ex vivo. Activated LPTC from IBD patients showed low proliferative rates and reduced secretion of TNF-α, IL-6, IFN-γ and IL-13 in the presence of L. kefiri. In addition, L. kefiri induced an increased frequency of CD4+FOXP3+ LPTC along with high levels of IL-10. This is the first report showing an immunomodulatory effect of L. kefiri CIDCA 8348 on human intestinal cells from IBD patients. Understanding the mechanisms of interaction between probiotics and immune mucosal cells may open new avenues for treatment and prevention of IBD.

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“…Probiotics may aid the modulation of the hosts' inflammatory status by reducing the cytokine secretion levels. The downregulation of proinflammatory cytokines such as IL-6 [45] and TNF-α [46,47] by probiotics has been reported on several occasions. Studies have shown that some peptides such as p40 and p75 secreted by Lactobaccillus rhamnosus GG (LGG) may prevent cytokine-induced apoptosis, increase heat-shock proteins, and could lead to the activation of mitogen-activated protein kinase (MAPK) [48].…”

Section: The Role Probiotics In Inflammatory Homeostasismentioning

confidence: 99%

Prebiotics, Probiotics and Synbiotic for Bone Health

Ilesanmi-Oyelere¹,

Kruger²

2022

Prebiotics and Probiotics - From Food to Health

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Prebiotics, probiotics and synbiotics has been shown to enhance calcium absorption, gut and bone health. Probiotics are also known to ferment prebiotics to produce the fermentative substrates such as short chain fatty acids (SCFAs), mainly acetate, butyrate and propionate with the help of beneficial micro-organisms in the gut. The expression of these SCFAs has been associated with the inhibition of osteoclast differentiation and bone resorption both in vitro and in vivo. In this review, we discuss the benefits of SCFAs and ways in which prebiotics and probiotics affect bone health by the reduction of inflammation in the gut and the bone.

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An evaluation of the effects of probiotics on tumoral necrosis factor (TNF-α) signaling and gene expression

Cited by 46 publications

References 85 publications

Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota

Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota

Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD

Prebiotics, Probiotics and Synbiotic for Bone Health

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