An experimental ovine Theileriosis: The effect of Theileria lestoquardi infection on cardiovascular system in sheep

Yaghfoori, Saeed; Razmi, Gholam Reza; Mohri, Mehrdad; Razavizadeh, A R Taghavi; Movassaghi, Ahmad Reza

doi:10.1016/j.actatropica.2016.05.014

Cited by 11 publications

(12 citation statements)

References 38 publications

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“…Similar findings have been reported in cattle (24). Increased cTnI concentrations have also been identified in other ruminant species with myocardial damage including goats (11,23) and sheep (3,12). In a study of histopathologically confirmed and morphometrically graded monensin toxicity in cattle, cTnI concentrations ranged from 0.4 to 39.0 ng/mL (median: 16.0 ng/mL) (24).…”

Section: Discussionsupporting

confidence: 74%

“…Cardiac troponin I (cTnI) is a sensitive and specific biomarker for the detection of myocardial injury in humans. In recent years cTnI has been evaluated in ruminant species with cardiac diseases such as pericarditis ( 1 ), endocarditis ( 2 , 3 ), as well as in neonates with congenital cardiac defects ( 4 , 5 ), non-cardiac disorders ( 6 , 7 ), nutritional muscular dystrophy ( 8 , 9 ), myocarditis ( 10 – 12 ), umbilical abscessation ( 13 ), envenomation ( 14 ), pregnancy toxemia ( 7 , 15 , 16 ), ruminal acidosis ( 17 , 18 ), and endotoxemia ( 19 ). Similarly, infection with Theileria annulata in cattle is associated with increased circulating cTnI ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury

Smith

Varga²,

Schober

2020

Front. Vet. Sci.

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Background: Multiple cardiac troponin I (cTnI) immunoassays are commercially available. Overall, assays have not been standardized, and inter-assay differences in the detection of the analyte cardiac troponin I can be clinically relevant. Objective: To compare the diagnostic accuracy of the commercially available Abbott i-STAT ® 1 cTnI immunoassay (i-STAT) and the previously validated ADVIA Centaur TnI-Ultra immunoassay (Centaur) in cattle. Hypothesis: There will be significant differences in bovine serum cTnI results measured by the Centaur and i-STAT methods. Animals: Ten dairy cows with experimentally induced myocardial injury due to monensin administration. Thirty apparently healthy dairy cows with no history of monensin exposure served as controls. Methods: Blood was collected at various time points after administration of a single dose of monensin (20 to 50 mg/kg) via orogastric tube. A total of 112 blood samples were collected. Cardiac TnI concentration was analyzed with the two methods and the association between methods analyzed via linear regression. Bland-Altman analysis to evaluate agreement between methods was performed on samples divided into groups (cTnI < 1.0 ng/mL and cTnI ≥ 1.0 ng/mL). Results: Analyzer results were linearly correlated with each other (R 2 = 0.931). Samples with cTnI concentrations <1.0 ng/mL had a bias of −0.13 ± 0.20 ng/mL and samples with cTnI concentrations >1.0 ng/mL had a bias of −9.81 ± 13.26 ng/mL. Conclusions and clinical importance: The results of this study reveal that cTnI concentrations determined with the i-STAT are systematically lower compared to the concentrations determined by the Centaur.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury

Smith

Varga²,

Schober

2020

Front. Vet. Sci.

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“…However, there is little information on infectious agents in livestock in Palestine, and the epidemiological aspects of theileriosis are not clearly understood, even though animal production is an important source of income in this country. So far, in a study done by Azmi and colleagues on ticks as hosts of pathogens in Palestine 8,9 , ovine species of Theileria were found in 5.4% of the ticks and were significantly associated with ticks from sheep and with the tick species Rhipicephalus turanicus . The aim of this study was to determine the prevalence of piroplasmid pathogens in domestic ruminants and equids from Palestine.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Theileria ovis and Theleiria equi in Livestock from Palestine

Azmi

Al-Jawabreh

Abdeen

2019

Sci Rep

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Theileria and Babesia are intracellular protozoan parasites infecting a wide range of animals. In Palestine, there is limited information on the prevalence of Theileria and Babesia spp. in livestock. We used PCR of the 18S ribosomal RNA gene followed by DNA sequencing to detect and identify parasite DNA in blood samples from sheep (n = 49), goats (n = 48), horses (n = 40), camels (n = 34), donkeys (n = 28) and mules (n = 2) from four districts of Palestine. DNA of T . ovis and T . equi was detected in 19 and 2 ovine blood samples, respectively. None of the camels, donkeys, and goats were positive for T . ovis . Sheep had a significantly higher rate of infection than other animals (P < 0.05). Theileria ovis is highly prevalent in sheep, while T . equi DNA was detected in a small proportion of the equids in Palestine.

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“…In this study, seven Baluchi sheep (4 females, 3 males) aged between 6 and 8 months were bought from a farm which had no history of theileriosis. The sheep were experimentally infected with T. lestoquardi as performed previously (Yaghfoori et al 2016, 2017). Briefly, infected adult H. anatolicum with T. lestoquardi were prepared at the Parasitology Department, Faculty of Veterinary Medicine of the Ferdowsi University of Mashhad, Iran.…”

Section: Methodsmentioning

confidence: 99%

The haematological, proinflammatory cytokines and IgG changes during an ovine experimental theileriosis

Razmi

Yaghfoori

Mohri

et al. 2019

Onderstepoort Journal of Veterinary Research

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Malignant ovine theileriosis is caused by Theileria lestoquardi , which is highly pathogenic in sheep. Theileriosis involves different organs in ruminants. Little is known about the role of proinflammatory cytokines in the pathogenesis of T. lestoquardi infection. The aim of this study was to measure concentration changes of proinflammatory cytokines and immunoglobulin G (IgG) during an ovine experimental theileriosis and correlate it with clinical and haematological parameters. During an experimental study, seven healthy Baluchi sheep (four females and three males) about 6–8 months old were infected with T. lestoquardi by feeding of infected unfed ticks on the sheep’s ears. The infected sheep were clinically examined during the study and blood samples were collected on days 0, 2, 5, 7, 10, 12, 14, 17 and 21. The haematological parameters were analysed by an automatic veterinary haematology cell counter and the inflammatory cytokines interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and IgG were measured by enzyme-linked immunosorbent assay. All infected sheep had temperatures above 40 °C on days 3–4 post infection (PI). The maximum temperature was noted on day 7, and it remained high until day 21. The parasitaemia of T. lestoquardi infection increased from 0.01% (day 7 PI) to 3.3% (day 21 PI). The mean white blood cell (WBC), red blood cell (RBC), lymphocyte, neutrophil and platelet values slightly increased on day 2 PI and decreased by day 17 and day 21 PI. The percentage parasitaemia and fever had a negative correlation with the numbers of WBCs, RBCs, lymphocytes, neutrophils and platelets. The serum concentration of IL-6, TNF-α and IFN-γ cytokines increased and peaked on day 12 and thereafter decreased to levels lower than 0. Out of all tested cytokines, the concentration of IL-6 was significantly higher, as early as day 2 PI. No significant changes were observed for the IgG levels during the course of disease. A significant and strong correlation was observed between IL-6, TNF-α and IFN-γ values and a moderate correlation between IL-6 and the numbers of lymphocytes in the present study. A strong correlation was determined between the percentage parasitaemia and haematological parameters in T. lestoquardi -infected sheep. In addition, preliminary results indicate that the measurement of the serum concentrations of IL-6 in combination with haematological parameters could be considered a good marker to estimate the pathogenicity of T. lestoquardi strain.

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An experimental ovine Theileriosis: The effect of Theileria lestoquardi infection on cardiovascular system in sheep

Cited by 11 publications

References 38 publications

Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury

Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury

Molecular Detection of Theileria ovis and Theleiria equi in Livestock from Palestine

The haematological, proinflammatory cytokines and IgG changes during an ovine experimental theileriosis

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