Molecular Detection of Theileria ovis and Theleiria equi in Livestock from Palestine

Azmi, Kifaya; Al-Jawabreh, Amer; Abdeen, Ziad

doi:10.1038/s41598-019-47965-0

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…T. ovis was more prevalent in sheep (38.9%) than in goats (0.8%). This finding is consistent with a study done on livestock in Palestine ( Azmi et al , 2019 ). Ovine theileriosis caused by T. ovis is among the most important infectious diseases affecting small ruminants, leading to significant economic losses to farmers ( Al-Hosary et al , 2021 ).…”

Section: Discussionsupporting

confidence: 93%

Molecular detection of novel Anaplasma sp. and zoonotic hemopathogens in livestock and their hematophagous biting keds (genus Hippobosca) from Laisamis, northern Kenya

Mwaki¹,

Kidambasi²,

Kinyua³

et al. 2022

Open Res Africa

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Background: Livestock are key sources of livelihood among pastoral communities. Livestock productivity is chiefly constrained by pests and diseases. Due to inadequate disease surveillance in northern Kenya, little is known about pathogens circulating within livestock and the role of livestock-associated biting keds (genus Hippobosca) in disease transmission. We aimed to identify the prevalence of selected hemopathogens in livestock and their associated blood-feeding keds. Methods: We randomly collected 389 blood samples from goats (245), sheep (108), and donkeys (36), as well as 235 keds from both goats and sheep (116), donkeys (11), and dogs (108) in Laisamis, Marsabit County, northern Kenya. We screened all samples for selected hemopathogens by high-resolution melting (HRM) analysis and sequencing of PCR products amplified using primers specific to the genera: Anaplasma, Trypanosoma, Clostridium, Ehrlichia, Brucella, Theileria, and Babesia. Results: In goats, we detected Anaplasma ovis (84.5%), a novel Anaplasma sp. (11.8%), Trypanosoma vivax (7.3%), Ehrlichia canis (66.1%), and Theileria ovis (0.8%). We also detected A. ovis (93.5%), E. canis (22.2%), and T. ovis (38.9%) in sheep. In donkeys, we detected ‘Candidatus Anaplasma camelii’ (11.1%), T. vivax (22.2%), E. canis (25%), and Theileria equi (13.9%). In addition, keds carried the following pathogens; goat/sheep keds - T. vivax (29.3%), Trypanosoma evansi (0.86%), Trypanosoma godfreyi (0.86%), and E. canis (51.7%); donkey keds - T. vivax (18.2%) and E. canis (63.6%); and dog keds - T. vivax (15.7%), T. evansi (0.9%), Trypanosoma simiae (0.9%), E. canis (76%), Clostridium perfringens (46.3%), Bartonella schoenbuchensis (76%), and Brucella abortus (5.6%). Conclusions: We found that livestock and their associated ectoparasitic biting keds carry a number of infectious hemopathogens, including the zoonotic B. abortus. Dog keds harbored the most pathogens, suggesting dogs, which closely interact with livestock and humans, as key reservoirs of diseases in Laisamis. These findings can guide policy makers in disease control.

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Section: Discussionsupporting

confidence: 93%

Molecular detection of novel Anaplasma sp. and zoonotic hemopathogens in livestock and their hematophagous biting keds (genus Hippobosca) from Laisamis, northern Kenya

Mwaki¹,

Kidambasi²,

Kinyua³

et al. 2022

Open Res Africa

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“…For example, Iqbal et al [11] reported 32 and a 5% prevalence of piroplasms in sheep and goats from the Punjab and KPK provinces of Pakistan, respectively. Likewise, Azmi et al [59] and Rjeibi et al [61] found higher prevalences of Theileria and piroplasms in sheep compared with goats from Palestine and Tunisia, respectively. Further molecular testing would be required to establish such differences, if any, as a smaller number of ticks were tested in the present study.…”

Section: Discussionmentioning

confidence: 92%

“…However, the prevalence of microorganisms was significantly higher in ticks collected from goats (83.9%) compared with those from sheep (56.5%) as shown in Table 3. Although there have been reports of higher prevalences of ticks [15] and internal parasites [58] in goats compared with sheep, most reports on the prevalence of haemoparasites in small ruminants from Pakistan and elsewhere are contrary to this finding [11,[59][60][61][62]. For example, Iqbal et al [11] reported 32 and a 5% prevalence of piroplasms in sheep and goats from the Punjab and KPK provinces of Pakistan, respectively.…”

Section: Discussionmentioning

confidence: 99%

An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan

et al. 2020

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This study investigated ticks and tick-borne microorganisms of small ruminants from five districts of the Federally Administered Tribal Area (FATA) of Pakistan. Morphological (n = 104) and molecular (n = 54) characterization of the ticks revealed the presence of six ixodid ticks: Rhipicephalus (Rh.) haemaphysaloides, Rh. microplus, Rh. turanicus, Haemaphysalis (Hs.) punctata, Hs. sulcata and Hyalomma anatolicum. Phylogenetic analyses of nucleotide sequence data for two mitochondrial (16S and cytochrome c oxidase 1) and one nuclear (second internal transcribed spacer) DNA regions provided strong support for the grouping of the six tick species identified in this study. Microfluidic real-time PCR, employing multiple pre-validated nuclear and mitochondrial genetic markers, detected 11 potential pathogens and endosymbionts in 72.2% of the ticks (n = 54) tested. Rickettsia (R.) massiliae was the most common pathogen found (42.6% of ticks) followed by Theileria spp. (33.3%), Anaplasma (A.) ovis and R. slovaca (25.9% each). Anaplasma centrale, A. marginale, Ehrlichia spp., R. aeschlimannii, R. conorii and endosymbionts (Francisella- and Coxiella-like) were detected at much lower rates (1.9–22.2%) in ticks. Ticks from goats (83.9%) carried significantly higher microorganisms than those from sheep (56.5%). This study demonstrates that ticks of small ruminants from the FATA are carrying multiple microorganisms of veterinary and medical health significance and provides the basis for future investigations of ticks and tick-borne diseases of animals and humans in this and neighboring regions.

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“…Detection of Babesia and Theileria spp. Two pairs of primers, PIRO-A/B ( 19 ) and BJ1/BN2 ( 3 ), targeting two fragments of the 18S rRNA gene were used to detect Babesia and Theileria spp., amplifying 408- and 487-bp fragments, respectively. A further PCR was performed using 2× Taq PCR Master Mix (Tiangen) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Detection of Babesia spp., Theileria spp., and Anaplasma ovis in Ornithodoros lahorensis from southern Xinjiang, China

Zhang

Zhou

et al. 2023

Journal of Veterinary Research

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Introduction Ticks are blood-sucking arthropods that have negative economic impacts and can spread a variety of diseases through their bites. There are few reports on soft ticks (Acari: Argasidae) and tick-borne pathogens in southern Xinjiang, China. This investigation supplements the available information for this region and is concerned with an argasid tick, apicomplexan parasites of the Babesia and Theileria genera and a bacterium of the Anaplasma genus. Material and Methods In this study, 330 soft ticks were collected from nine sampling sites in southern Xinjiang between 2020 and 2021. The ticks were identified according to their morphological characteristics and confirmed as Ornithodoros lahorensis using mitochondrial 16S rDNA sequences. Babesia and Theileria were identified at the species level based on two fragments of the 18S rRNA gene, and one set of primers targeting the 16S rRNA gene was used to identify the Anaplasma genus. Results Among the 330 samples, one Babesia species (Babesia sp.), two Theileria species (T. ovis and T. annulata), and one Anaplasma (A. ovis) species were detected. Conclusion This study provides fundamental evidence for the occurrence of Babesia, Theileria and Anaplasma spp. in soft ticks. To the best of our knowledge, this is the first report of the detection of Babesia sp. and T. annulata in O. lahorensis. Therefore, the potential threat of soft ticks to livestock and humans should not be ignored.

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Molecular Detection of Theileria ovis and Theleiria equi in Livestock from Palestine

Cited by 11 publications

References 23 publications

Molecular detection of novel Anaplasma sp. and zoonotic hemopathogens in livestock and their hematophagous biting keds (genus Hippobosca) from Laisamis, northern Kenya

Molecular detection of novel Anaplasma sp. and zoonotic hemopathogens in livestock and their hematophagous biting keds (genus Hippobosca) from Laisamis, northern Kenya

An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan

Detection of Babesia spp., Theileria spp., and Anaplasma ovis in Ornithodoros lahorensis from southern Xinjiang, China

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