An Ice-Solvent Method of Drying Frozen Tissue for Plant Cytology

Myelin 212 Chapter 16. Hematologic Elements and Related Tissues . Blood Smears Blood Tissue Elements and Inclusion Bodies .... Bone Marrow Staining Staining for Fibrin Chapter 17. Pigments and Minerals Testing for Iron Testing for Calcium Hemoglobin Staining Bile Pigment Staining Melanin and Lipofuscin Staining 241 Removal of Pigments Chapter IS. Cytoplasmic Elements Protein Staining The Argentaffin Reaction Fat Staining Golgi Apparatus and Mitochondria Contents xiii Saccharides (carbohydrates) 266 Mucin Staining 273 Amyloid Staining 274 Mast Cell Staining 276 Metachromasia 278 Pituitary and Pancreas 283 Chapter 19. Feulgen and PAS Technics and Related Reactions 292 Feulgen Reaction 293

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“…Meryman (1959,1960); Packer and Scott (1942); Patten and Brown (1958); Simpson (1941); and Woods and Pollister (1955).…”

Section: Ice-solvent (Freeze-substitution) Methodsmentioning

confidence: 99%

Animal tissue techniques

Humason¹

1962

1,796

789

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“…Seeds from a stock with 25 B-chromosomes were also used.' After germination on wet filter paper until the roots were 4-8 mm in length, the root tips were removed and fixed either in 10% neutral formalin or by freezesubstitution (30), or they were placed in isotope for suitable periods of time and then removed and fixed similarly. The root tips were embedded in paraffin and sectioned at 4 or 20 /p.…”

Section: Methodsmentioning

confidence: 99%

An Analysis of Heterochromatin in Maize Root Tips

Himes

1967

The Journal of Cell Biology

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The B chromosomes of maize are condensed in appearance during interphase and are relatively inert genetically; therefore they fulfill the definition of heterochromatin. This heterochromatin was studied in root meristem cells by radioautography following administration of tritiated thymidine and cytidine, and was found to behave in a characteristic way, i.e. it showed asynchronous DNA synthesis and very low, if any, RNA synthesis. A cytochemical comparison of normal maize nuclei with nuclei from isogenic maize stock containing approximately 15-20 B-chromosomes in addition to the normal complement has revealed the following: (a) the DNA and histone contents are greater in nuclei with B chromosomes; (b) the proportion of DNA to histone is identical with that of nuclei containing only normal chromosomes; (c) the amount of nonhistone protein in proportion to DNA in interphase is less in nuclei with B chromosomes than in normal nuclei. In condensed B chromosomes the ratio of nonhistone protein to DNA is similar to that in other condensed chromatin, such as metaphase chromosomes and degenerating nuclei. The B chromosomes appear to have no effect on nucleolar RNA and protein. Replication of B chromosomes is precisely controlled and is comparable to that of the ordinary chromosomes not only in synthesis for mitosis but also in formation of polyploid nuclei of root cap and protoxylem cells.

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“…The animals were sacrificed at specific intervals following hormonal treatment, and their livers were removed and sliced at a thickness of less than 2 mm . These slices were processed by the freeze substitution method of Woods and Pollister (14), with methanol as the dehydrating medium followed by hot methanol (60 °C) treatment for 1 hr . At the end of the fixation periods, the tissues were embedded in paraffin and sectioned at the appropriate thickness required by the method of analysis employed .…”

Section: Methodsmentioning

confidence: 99%