Myelin 212 Chapter 16. Hematologic Elements and Related Tissues . Blood Smears Blood Tissue Elements and Inclusion Bodies .... Bone Marrow Staining Staining for Fibrin Chapter 17. Pigments and Minerals Testing for Iron Testing for Calcium Hemoglobin Staining Bile Pigment Staining Melanin and Lipofuscin Staining 241 Removal of Pigments Chapter IS. Cytoplasmic Elements Protein Staining The Argentaffin Reaction Fat Staining Golgi Apparatus and Mitochondria Contents xiii Saccharides (carbohydrates) 266 Mucin Staining 273 Amyloid Staining 274 Mast Cell Staining 276 Metachromasia 278 Pituitary and Pancreas 283 Chapter 19. Feulgen and PAS Technics and Related Reactions 292 Feulgen Reaction 293

Histological sections of colons from 69 tamarins (46 Saguinus oedipus and 23 Saguinus fuscicollis illigeri) and 27 marmosets (Callithrix jacchus) that died between 1979 and 1984 were examined for colitis. Evaluated biological factors were species, age at death, source of animals, manner of death, presence of colon cancer, and time after importation. Most normal colons were found in young animals (dead at less than 1 years of age). Nearly all (approximately 96%) animals had colitis; 70-80% of most groups were graded as chronic colitis. Usually, one grade adequately described the condition of the entire colon. The strongest observed correlation of factors (P less than 0.05) was between acute colitis and colon cancer in S. oedipus. A higher percentage of S. oedipus had acute colitis than did the other two species. When colitis incidence data were adjusted for S. oedipus with colon cancer, there were no observed species differences between colons of colony-born and imported animals nor between those that died naturally and those that were euthanized. In an additional group of 18 S. oedipus that were imported in 1975, acute colitis was found in 60% of those dying immediately after importation (less than 1 year of colony age) and those that survived greater than 3 years. At this time, no causative agent has been identified in marmoset colitis.

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