Animal tissue techniques

Humason, Gretchen L.

doi:10.5962/bhl.title.5890

Cited by 1,796 publications

(869 citation statements)

References 46 publications

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“…Histology The histology sections of the kidneys of male rats were taken by adopting the procedure as described by Humason (1972). The tissues were isolated and gently rinsed with saline solution to remove mucus and other debris adhering to them.…”

Section: Methodsmentioning

confidence: 99%

Ameliorating activity of ginger (Zingiber officinale) extract against lead induced renal toxicity in male rats

et al. 2011

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Lead poisoning has been known to be associated with structural and functional abnormalities of multiple organ systems of human body. The aim of this investigation was to study the renal protective effects of ginger (Zingiber officinale) extract in lead induced toxicity rats. In this study renal glutathione (GSH) level, glutathione peroxidase (GPX), glutathione-s-transferase (GST), and catalase enzymes were measured in lead nitrate (300 mg/kg BW), and lead nitrate plus ginger extract (150 mg/kg BW) treated rat groups for 1 week and 3 weeks respectively. The glutathione level and GSH dependent antioxidant enzymes such as glutathione peroxidase, glutathione-stransferase, and catalase significantly (P<0.05) increased in ginger extract treated rat groups. In addition, histological studies showed lesser renal changes in lead plus ginger extract treated rat groups than that of lead alone treated rat groups. These results indicate that ginger extract alleviated lead toxic effects by enhancing the levels of glutathione, glutathione peroxidase, glutathiones-transferase and catalase.

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Section: Methodsmentioning

confidence: 99%

Ameliorating activity of ginger (Zingiber officinale) extract against lead induced renal toxicity in male rats

et al. 2011

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“…The experimental limbs that developed at the site of transplantation were excised and fixed in Zenker's fixative for 2 hr or overnight depending on the size of the tissue. The fixed limbs were embedded in paraffin, serially sectioned at 6 pm and stained with Feulgen stain (Humason, 1972). Feulgen staining allowed the identification of quail from chick cells and tracing of the fate of the quail cells in the resulting limb.…”

Section: Histologymentioning

confidence: 99%

Cellular contribution of the different regions of the somatopleure to the developing limb

Geduspan

Solursh

1992

Developmental Dynamics

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Regionalization of the presumptive limb region was examined before and at the onset of limb development by means of a variety of transplantation experiments between quail and chick embryos in ovo. The results demonstrate a two-step process, the first of which is the designation of the region of the somatopleure that would become part of the limb, followed by specification of dorsal and ventral regions of the limb. The medial half of the somatic mesoderm is the region which gives rise to the limb with only a smaller cellular contribution from the lateral half of the somatic mesoderm. The cellular contribution of the medial region of the somatopleure appeared to determine the type of limb formed (i.e,. wing or leg). The second process relates to changes in the ability of the somatic ectoderm to undergo extensive lateral displacement with development. Starting at stage 14, the medial and lateral somatic ectoderms maintain their position after transplantation, in contrast to earlier stage limb or flank ectoderms which undergo extensive lateral displacement with development. The positional determination of the dorsal and ventral properties of the medial and lateral ectoderms of the prospective limb region and their distal displacement during limb outgrowth may be important morphogenetic events in limb development.C 1993 Wiley-Liss, Inc.

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“…Each specimen was oriented and embedded in Paraplast Plus within a Peel-a-way paraffin embedding molds (Fisher Scientific, Pittsburgh, PA), and the wax was allowed to harden. Specimens were then sectioned at either 10 m thickness for hematoxylin staining or 20 m for better visualization of in situ staining, and the sections were mounted on albumin-coated slides (Humason, 1972).…”

Section: Histological Preparationsmentioning

confidence: 99%

“…The slides were stained by 3-sec immersion in Harris' hematoxylin (Fisher Scientific, Pittsburgh, PA), and the stain was cured in running tap water and Scott's solution (after Humason, 1972). After staining, the slides were dehydrated through graded ethanol washes to final xylene washes, after which coverslips were mounted using Permount medium (Sigma).…”

Section: Histological Preparationsmentioning

confidence: 99%

Isolation and characterization of a novel gene, xMADML, involved in Xenopus laevis eye development

Elkins

Henry

2006

Developmental Dynamics

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We have identified Xenopus MADM-like (xMADML), a Xenopus laevis gene related to the murine MADM and the human NRBP genes. xMADML is expressed throughout early development and is expressed most strongly in the developing lens and more weakly in the retina and other anterior tissues. We demonstrate that disruption of xMADML translation by means of morpholino injection results in impaired retina and lens development. Reciprocal transplantation of the presumptive lens ectoderm between morpholinoinjected embryos and those injected solely with a dextran lineage tracer demonstrates that xMADML is necessary in both the lens and the retina for correct development of these eye tissues. Analysis of gene expression after knockdown of xMADML revealed significant alterations in the expression of some genes, including Pax6, xSix3, Sox2, and Sox3, suggesting that xMADML plays a role in regulating gene expression during development of the eye. This investigation is the first in vivo study examining the developmental role of this novel gene and reveals an important role of xMADML in eye tissue development and differentiation.

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Animal tissue techniques

Cited by 1,796 publications

References 46 publications

Ameliorating activity of ginger (Zingiber officinale) extract against lead induced renal toxicity in male rats

Ameliorating activity of ginger (Zingiber officinale) extract against lead induced renal toxicity in male rats

Cellular contribution of the different regions of the somatopleure to the developing limb

Isolation and characterization of a novel gene, xMADML, involved in Xenopus laevis eye development

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