An image analysis method for the study of cell adhesion to biomaterials

Dubois, Jean‐Christophe; Souchier, Catherine; Couble, Marie‐Lise; Exbrayat, Patrick; Lissac, M.

doi:10.1016/s0142-9612(99)00082-4

Cited by 29 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar responses to this current study have been observed by Dubois and Souchier (1999) who found enstatite (Mg 2 Si 2 O 3 ) and TCP were shown to support the greatest bone cell adhesion compared with glass after 2 h. Okumura and Goto (2001) demonstrated HA to support a greater cell attachment at 6, 9, 12 and 24 h culture than Ti and glass using Saos-2 human osteosarcoma cells. They also measured Saos-2 cell-areas and found no significant difference between HA, Ti and glass surfaces after 3 h; however cell areas rapidly increased upon HA between 3 and 12 h then reached a plateau.…”

Section: Discussionsupporting

confidence: 89%

Human osteoblast cell spreading and vinculin expression upon biomaterial surfaces

et al. 2007

View full text Add to dashboard Cite

Any biomaterial implanted within the human body is influenced by the interactions that take place between its surface and the surrounding biological milieu. These interactions are known to influence the tissue interface dynamic, and thus act to emphasize the need to study cell-surface interactions as part of any biomaterial design process. The work described here investigates the relationship between human osteoblast attachment, spreading and focal contact formation on selected surfaces using immunostaining and digital image processing for vinculin, a key focal adhesion component. Our observations show that a relationship exists between levels of cell attachment, the degree of vinculin-associated plaque formation and biocompatibility. It also suggests that cell adhesion is not indicative of how supportive a substrate is to cell spreading, and that cell spreading does not correlate with focal contact formation.

show abstract

Section: Discussionsupporting

confidence: 89%

Human osteoblast cell spreading and vinculin expression upon biomaterial surfaces

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Features such as dimensions, surface properties, characteristics of the recipient site, trauma during surgery and movement of the bone/implant interface should be taken into consideration when the efficacy of osseointegration of the material is determined 30 . Histological analysis of the defect areas in the groups receiving the membranes showed little bone formation from the margins of the defect, the absence of bone encasement of the graft, and the marked presence of connective tissue adjacent to the membrane and at the bone/implant interface, decharacterizing osseointegration of the biomaterial as reported in other research 31 .…”

Section: Macroscopic and Radiological Analysismentioning

confidence: 99%

In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

et al. 2017

View full text Add to dashboard Cite

Biomaterials with the hydroxyapatite and biopolymers such as chitosan derived of crustaceans are is an alternative for bone repair. Carbon nanotubes have been a focus of interest because they can ameliorate the biomechanical properties of biomaterials. The objective of this study was to evaluate these materials in the repair of cranial defects in rats. The animals were divided in groups: without implant (G1), implanted with the chitosan/carbon nanotube membrane (G2), and chitosan/nanotube membrane mineralized with hydroxyapatite (G3). The animals were sacrificed 5 weeks after surgery and the skulls were removed for analysis of the defect area. The results showed absence of chronic inflammatory and little bone neoformation in the defect area of all groups. In G2 and G3 there was lack of reabsorption of the biomaterial that were encapsulated by connective tissue. In conclusion, the biomaterials were biocompatible, but their specific physicochemical properties did not indicate a considerable osteoregenerative capacity.

show abstract

“…A QCM system with a micro CCD camera, which can observe the cell structure changes with CCD camera and measure the viscoelastic characteristics of the cell with the QCM measurement simultaneously, was developed to investigate the effects of chemical stressors on the cultured cells. Understanding the characteristics of the cultured cells for both the viscoelastic characteristics of the cultured cell and the cell shape is important because both of them are important factors for the cell activity [99,100] and the cell adhesive function [101,102]. The viscoelastic characteristics of the cultured cell could be analyzed using the resonant frequency and resonant resistance diagram.…”

Section: Extension Of the Quartz Crystal Application Coupled With Othmentioning

confidence: 99%

The principles and applications of nano-diagnosis system for a nano-biosensor

Kim

Chang

Muramatsu

et al. 2011

Korean J. Chem. Eng.

View full text Add to dashboard Cite

In this review, the basic principles and research trends of biosensors are briefly described and a nano-sensing system applying QCM (quartz crystal micro-balance), nano-diagnosis methods by AFM (atomic force microscopy) and SNOAM (scanning near-field/atomic force microscopy) is discussed intensively. The principle, construction, and applications of piezoelectric crystal sensors as a universal sensor are reviewed. This review is focused mainly on liquid phase applications, such as immune-sensors, gelation detecting sensors, and cultured cell monitoring sensors. The principle of nano-diagnosis based on the AFM or SNOAM techniques is described in detail. Finally, the binding affinity of peptide probes to proteins using AFM and the visualizing of a hybridized PNA probe on a DNA molecule using SNOAM are evaluated and discussed.

show abstract

An image analysis method for the study of cell adhesion to biomaterials

Cited by 29 publications

References 43 publications

Human osteoblast cell spreading and vinculin expression upon biomaterial surfaces

Human osteoblast cell spreading and vinculin expression upon biomaterial surfaces

In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

The principles and applications of nano-diagnosis system for a nano-biosensor

Contact Info

Product

Resources

About