An immunoassay for histamine based on monoclonal antibodies

To study the usefulness of urinary 1-methylhistamine and serum tryptase concentration as monitoring parameters in clinical settings, we investigated 32 children with atopic dermatitis and suspected food allergy during oral food challenges with eggs and cow’s milk. Urinary 1-methylhistamine (MH) excretion increased significantly within lh after positive oral food challenges (p < 0.006), but showed considerable variation in negative challenges. MH seems to be a sensitive parameter (92.8%), but its specifity is insufficient (37.7%). In the group of 16 positive oral food challenges serum tryptase concentration increased significantly (p < 0.02) directly after provocation and remained elevated up to 2 h after provocation. No variation was observed in negative challenges or nonatopic controls. Serum tryptase concentration seems to be specific for marked clinical reactions after oral food challenges (100%), but its sensitivity was low (25%) and not superior to evaluation by clinical means. We conclude that, despite positive results for the group of children, MH and serum tryptase concentrations are not useful parameters for monitoring oral food challenges in an individual child due to insufficient sensitivity and specificity.

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“…Urinary MH was determined by a radioimmunoassay (Pharmacia, Uppsala, Sweden) [13]. Detection limit o f the assay was 4 ninol/t.…”

Section: Methodsmentioning

confidence: 99%

Serum Tryptase and Urinary 1-Methylhistamine as Parameters for Monitoring Oral Food Challenges in Children

Beyer¹,

Niggemann²,

Schulze³

et al. 1994

Int Arch Allergy Immunol

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“…Histamines and their metabolites can be determined in body fluids by various sensitive and specific methods; mainly using the immunoassay technique [83]. Detection in plasma or urine is not important for diagnosis of allergic diseases because of the rapid metabolism in organisms and influence of several variables.…”

Section: Diagnostic Methods For Early Diagnosis Of Allergiesmentioning

confidence: 99%

Early detection of allergic diseases in otorhinolaryngology

Klimek

Schendzielorz²

2008

GMS Current Topics in Otorhinolaryngology - Head and Neck Surgery; 7:Doc04; ISSN 1865-1011

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“…Therefore, the present study describes the capability of a given organ to synthesize histamine, but not necessarily the production of biologically active, i.e., extracellularly released histamine. A compelling advantage of this study was the use of HPLC-coupled tandem mass spectrometry, which, in comparison to former methods of histamine quantification (bioassay [8], fluorometry [9], immunoassay [10], GC-MS [11]), is highly sensitive and highly specific. In addition, this method allowed the parallel measurement of N-methylhistamine, the main metabolite of histamine [12], which is generated by the activity of the enzyme histamine-N-methyltransferase.…”

Section: General Aspectsmentioning

confidence: 99%

Systematic analysis of histamine and N-methylhistamine concentrations in organs from two common laboratory mouse strains: C57Bl/6 and Balb/c

et al. 2011

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HPLC-mass spectrometry is a useful method for the highly sensitive and simultaneous detection of histamine and N-methylhistamine. Histamine is present in virtually all organs, not only in those traditionally associated with histamine-mediated disease. Highest concentrations are found in stomach, lymph node, and thymus; medium concentrations in heart, spleen, and kidney; and lowest concentrations detected in intestine, brain, liver, and lung.

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An immunoassay for histamine based on monoclonal antibodies

Cited by 36 publications

References 13 publications

Serum Tryptase and Urinary 1-Methylhistamine as Parameters for Monitoring Oral Food Challenges in Children

Serum Tryptase and Urinary 1-Methylhistamine as Parameters for Monitoring Oral Food Challenges in Children

Early detection of allergic diseases in otorhinolaryngology

Systematic analysis of histamine and N-methylhistamine concentrations in organs from two common laboratory mouse strains: C57Bl/6 and Balb/c

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