An immunohistochemical study of the distribution of biotin in tissues of pigs and chickens

Cooper, K; Kennedy, Susan; McConnell, S.; Kennedy, Denise; Frigg, M.

doi:10.1016/s0034-5288(97)90024-2

Cited by 20 publications

(19 citation statements)

References 21 publications

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“…Endocrine islets show different distributions, locations, and characteristics among avian species [1] . A high number of alpha-, beta-, and somatostatin-releasing cells, and a small number of gastrin-, serotonin-, substance P-, neuropeptide Y-, and cholecystokinin-8-releasing cells are located in the pancreatic endocrine islets of some avian species [3,5,6,[12][13][14]16] . Therefore, the present study aimed to investigate the distribution and location of endocrine cells in the pancreas of the sparrowhawk.…”

Section: Discussionmentioning

confidence: 99%

“…The distributions of the different types of cells in the pancreas of avian species have been demonstrated by immunohistochemical methods in mynah [12] , chickens [13] , falcons [7] , ducks [3] , geese [14] , and young Japanese quails [5] . To our knowledge, there is no study showing the histological distribution of endocrine cells in the pancreas of the sparrowhawk (Accipiter nisus).…”

Section: Introductionmentioning

confidence: 99%

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Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Kara¹,

Tekiner²,

Şimşek³

et al. 2014

Kafkas Univ Vet Fak Derg

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The distribution and location of endocrine cells were determined in the dorsal, ventral, and splenic lobes of sparrowhawk pancreas using immunohistochemical methods. In this study, pancreatic tissues were stained with anti-insulin, anti-glucagon, anti-somatostatin, anti-gastrin, and anti-serotonin primary antibodies using the streptavidin-biotin-peroxidase method. The results showed that the numbers of glucagon-(alpha cell), insulin-(beta cell), and somatostatin-(delta cell) releasing cells were high and located in the splenic lobe of the pancreas. These endocrine cells were grouped into alpha, beta, and mixed islets. Alpha islets were mainly composed of alpha and delta cells and also occasionally beta cells. Beta islets contained numerous beta cells and a few delta and alpha cells. Furthermore, in the exocrine tissue were showed as only one cell or 2-3 gastrin immunopositive cell groups, whereas serotonin immunopositive cells were not found anywhere in the exocrine and endocrine pancreas. In conclusion, the endocrine islet types, endocrine cell localizations and lobe numbers of pancreas in sparrowhawks are similar to predator bird species, but are determined different to domestic fowls. Keywords: Insulin, Glucagon, Somatostatin, Gastrin, Pancreas, Sparrowhawk Atmaca (Accipiter nisus) Pankreas Dokusu Endokrin Hücrelerin Dağılımı ve Lokalizasyonu ÖzetBu çalışmada, atmaca pankreasının dorsal, ventral ve splenik loblarında bulunan endokrin hücrelerin dağılımı immunhistokimysal metotlarla belirlenmiştir. Pankreas dokusu anti-glukagon, anti-insulin, anti-gastrin ve anti-somatostatin primer antikorları kullanılarak streptavidin-biotin peroxidase metodu ile boyandı. Çalışmada, glukagon (alfa hücresi), insülin (beta hücresi) ve somatostatin (delta hücresi) salgılayan hücrelerin splenik lobda sayısının en fazla olduğu saptandı. Atmalarda pankreasın endokrin bölümlerinin alfa, beta ve miks adacıklar halinde gruplaştıkları saptandı. Beta adacıklarının çok sayıda beta hücresi, az sayıda delta ve alfa hücrelerini içerdiği belirlendi. Alfa adacıkları, genellikle alfa ve delta hücrelerinden nadiren de beta hücrelerinden oluşuyordu. Ayrıca, gastrin pozitif hücreler, sadece ekzokrin pankreasta 1 ya da 2-3 adet hücre grubu halinde bulunurken, seratonin pozitif hücrelere ise ekzokrin ve endokrin pankreasın herhangi bir bölümünde rastlanmadı. Sonuç olarak, atmaca pankreasının lop sayısı, endokrin adacık tipleri ve endokrin hücre lokalizasyonu açısından etçil kuşlarla benzerliği, evcil kuş türleriyle de farklılıkları belirlenmiştir.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Kara¹,

Tekiner²,

Şimşek³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…These studies identified a few oligodendrocytes in tract systems (LeVine and Macklin, 1988) and intermittent labeling of neurons in hippocampus (Wang and Pevsner, 1999). Biotin labeling has also been reported in cerebellar Purkinje cells, although this was in pigs fed a biotin-supplemented diet (Cooper et al, 1997). These latter results are not easily compared with endogenous biotin in rats maintained on a normal diet, as artificial increases in biotin (as exemplified by ventricular injection of biocytin) leads to widespread labeling of cells that are ordinarily biotin-negative (McDonald et al, 1992;Ma, 1994).…”

Section: Biotin Is Selectively Distributed In Cnsmentioning

confidence: 99%

Biotin is endogenously expressed in select regions of the rat central nervous system

McKay

Molineux

Turner

2004

J of Comparative Neurology

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The vitamin biotin is an endogenous molecule that acts as an important cofactor for several carboxylases in the citric acid cycle. Disorders of biotin metabolism produce neurological symptoms that range from ataxia to sensory loss, suggesting the presence of biotin in specific functional systems of the CNS. Although biotin has been described in some cells of nonmammalian nervous systems, the distribution of biotin in mammalian CNS is virtually unknown. We report the presence of biotin in select regions of rat CNS, as revealed with a monoclonal antibody directed against biotin and with avidin- and streptavidin-conjugated labels. Detectable levels of biotin were primarily found caudal to the diencephalon, with greatest expression in the cerebellar motor system and several brainstem auditory nuclei. Biotin was found as a somatic label in cerebellar Purkinje cells, in cell bodies and proximal dendrites of cerebellar deep nuclear neurons, and in red nuclear neurons. Biotin was detected in cells of the spiral ganglion, somata and proximal dendrites of cells in the cochlear nuclei, superior olivary nuclei, medial nucleus of the trapezoid body, and nucleus of the lateral lemniscus. Biotin was further found in pontine nuclei and fiber tracts, the substantia nigra pars reticulata, lateral mammillary nucleus, and a small number of hippocampal interneurons. Biotin was detected in glial cells of major tract systems throughout the brain but was most prominent in tracts of the hindbrain. Biotin is thus expressed in select regions of rat CNS with a distribution that correlates to the known clinical sequelae associated with biotin deficiencies.

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“…With the exception of a single report that biotinidase localizes to the nucleus [31], most other studies have neglected to examine the nucleus for biotinidase activity even though there is strong evidence that biotin localizes to the nucleus in biotin-deficient states [32]. It was not until our report that biotinidase can biotinylate histones [7], which has been recently confirmed by others [33,34], that a possible role for biotinidase in the nucleus was considered.…”

Section: The Cell Biology Of Biotinidasementioning

confidence: 63%

Biotinidase: its role in biotinidase deficiency and biotin metabolism

Wolf

2005

The Journal of Nutritional Biochemistry

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An immunohistochemical study of the distribution of biotin in tissues of pigs and chickens

Cited by 20 publications

References 21 publications

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Biotin is endogenously expressed in select regions of the rat central nervous system

Biotinidase: its role in biotinidase deficiency and biotin metabolism

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