An impedimetric biosensor for COVID-19 serology test and modification of sensor performance via dielectrophoresis force

Zeng, Jie; Duarte, Pedro Augusto Silva; Ma, Yuhao; Savchenko, Oleksandra; Shoute, Lian C. T.; Khaniani, Yeganeh; Babiuk, Shawn; Zhuo, Ran; Abdelrasoul, Gaser N.; Charlton, Carmen; Kanji, Jamil N; Babiuk, Lorne; Edward, Cole; Chen, Jie

doi:10.1016/j.bios.2022.114476

Cited by 31 publications

(18 citation statements)

References 52 publications

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“…Advanced optical sensor technologies (Table 3) such as photonic [27] and surface plasmon resonance sensors [28,29] are able to provide results within 10 min, whereas more traditional approaches require more time (LFA, ELISA, 15-60 min) [30,31].On the other hand, electrochemical sensors require a mean response time of approximately 30 min [32][33][34][35][36]. Taking into account the above-mentioned, our biosensor is able to detect SARS-CoV-2 antibodies in an ultra-rapid manner (3 min) and at quite low concentrations (5 ng/mL).…”

Section: Discussionmentioning

confidence: 99%

Ultra-Fast and Sensitive Screening for Antibodies against the SARS-CoV-2 S1 Spike Antigen with a Portable Bioelectric Biosensor

et al. 2022

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As a consequence of the progress of the global vaccination against the COVID-19 disease, fast, accurate and affordable assays are needed for monitoring the efficiency of developing immunity against the coronavirus at the population level. In this context, we herewith report the proof-of-concept development of an innovative bioelectric biosensor for the ultra-detection (in less than three minutes) of IgG antibodies against the SARS-CoV-2 S1 spike antigen. The biosensor comprises a disposable set of screen-printed electrodes upon which are immobilized cells engineered to bear the S1 protein on their surface. When anti-S1 antibodies are presented to the engineered cell population, a rapid, specific, and selective change of the cell membrane potential occurs; this is in turn recorded by a bespoke portable potentiometer. End results are communicated via Bluetooth to a smartphone equipped with a customized user interface. By using the novel biosensor, anti-S1 antibodies could be detected at concentrations as low as 5 ng/mL. In a preliminary clinical trial, positive results were derived from patients vaccinated or previously infected by the virus. Selectivity over other respiratory viruses was demonstrated by the lack of cross-reactivity to antibodies against rhinovirus. After further clinical validation and extension to also screen IgM, IgA and possible neutralizing antibodies, our approach is intended to facilitate the mass and reliable detection of antibodies in the early stages following vaccination and to monitor the duration and level of acquired immunity both in a clinical and self-testing environment.

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Section: Discussionmentioning

confidence: 99%

Ultra-Fast and Sensitive Screening for Antibodies against the SARS-CoV-2 S1 Spike Antigen with a Portable Bioelectric Biosensor

et al. 2022

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“…biosensors are theoretically favoured for POC diagnostics and device integration because they can directly detect biorecognition events by measuring the non-faradaic resistance and capacitance properties of the sensing electrode; however, their practical implementation suffers from non-specific binding of non-target compounds, which leads to low sensitivity and selectivity. In addition, although impedimetric biosensors have been greatly improved during the COVID-19 pandemic, for example, using molecular imprinting technology to fabricate virusimprinted impedimetric biosensors for sensitive detection of whole virus particles 22 or by applying a dielectrophoresis force to improve the detection sensitivity of impedimetric immunosensors fabricated on Au micro-interdigitated electrodes 23 , their proof-of-concept performance is often only demonstrated using artificial physiological samples instead of clinically relevant samples.…”

Section: Review Articlementioning

confidence: 99%

Device integration of electrochemical biosensors

et al. 2023

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Electrochemical biosensors incorporate a recognition element and an electronic transducer for the highly sensitive detection of analytes in body fluids. Importantly, they can provide rapid readouts and they can be integrated into portable, wearable and implantable devices for point-of-care diagnostics; for example, the personal glucose meter enables at-home assessment of blood glucose levels, greatly improving the management of diabetes. In this Review, we discuss the principles of electrochemical biosensing and the design of electrochemical biosensor devices for health monitoring and disease diagnostics, with a particular focus on device integration into wearable, portable and implantable systems. Finally, we outline the key engineering challenges that need to be addressed to improve sensing accuracy, enable multiplexing and one-step processes, and integrate electrochemical biosensing devices in digital health-care pathways.

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“…5B shows the concepts, electrode modification, immunosensor, and the corresponding The biosensor comprised gold micro-interdigitated electrodes and viral Spike-S proteins from the virus that were coated between the electrode digits to trap anti-S antibodies in the samples. 146 The work introduced two approaches to improve the sensitivity of the impedimetric sensor, including the use of secondary antibody-gold nanoparticles (AuNPs) and dielectrophoresis force to concentrate the target antibody. The AuNP approach reached a LoD of 200 ng mL −1 , while the DEP method had a LoD of 2 μg mL −1 but had a faster testing time of only 30 minutes.…”

Section: Electrochemical Impedance Biosensor Applicationsmentioning

confidence: 99%

Concepts, electrode configuration, characterization, and data analytics of electric and electrochemical microfluidic platforms: a review

Nguyen

Thi

et al. 2023

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An impedimetric biosensor for COVID-19 serology test and modification of sensor performance via dielectrophoresis force

Cited by 31 publications

References 52 publications

Ultra-Fast and Sensitive Screening for Antibodies against the SARS-CoV-2 S1 Spike Antigen with a Portable Bioelectric Biosensor

Ultra-Fast and Sensitive Screening for Antibodies against the SARS-CoV-2 S1 Spike Antigen with a Portable Bioelectric Biosensor

Device integration of electrochemical biosensors

Concepts, electrode configuration, characterization, and data analytics of electric and electrochemical microfluidic platforms: a review

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