An Improved Screening Method for Microorganisms Able to Convert Crude Glycerol to 1,3-Propanediol and to Tolerate High Product Concentrations

Ringel, Anne Katrin; Mildner, E.; Willke, Thomas; Böttcher, Dominique; Schubert, M.; Vorlop, Klaus‐Dieter

doi:10.1016/j.jbiotec.2010.09.557

Cited by 2 publications

(4 citation statements)

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“…Compared with other microbial producing method for 1,3-propanediol using crude glycerol [12,13] or two-stage from glucose [14,15], the yield is much lower. To our knowledge, no related study on producing 1,3-propanediol from glucose using a mixed culture of these two species has been reported before.…”

Section: Discussionmentioning

confidence: 97%

“…Compared with chemical synthesis, fermentation has the advantage of mass production at low cost, and has been used in production of various industrial chemicals [3]. For example, the glycerol broth from the yeast fermentation and biodiesel-derived raw glycerol as the substrate have been used for 1,3-propanediol production [7][8][9][10][11][12][13]. In nature, a few microorganisms such as Klebsiella [4], Citrobacters [5], and Clostridium [6] were reported to produce 1,3-propanediol, but no natural microorganism has been found to accumulate 1,3-propanediol directly from the low-cost feedstock.…”

Section: Introductionmentioning

confidence: 99%

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1,3‐propanediol production from glucose by mixed‐culture fermentation of Zygosacharomyces rouxii and Klebsiella pneumonia

Ma¹,

Shentu²,

Bian³

et al. 2012

Engineering in Life Sciences

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1,3‐propanediol is an important chemical widely used in polymer production. In this study, two strains, Zygosacharomyces rouxii JL2011 and Klebsiella pneumoniae S6, were used as a mixed culture for 1,3‐propanediol production directly from glucose. Two important parameters including inoculation time of K. pneumoniae S6 at stage of mixed culture and initial cell ratio of Z. rouxii JL2011 to K. pneumoniae S6 in mixed fermentation were optimized in culture flasks. In those experiments, the best results were obtained with a yield of 6.8 g/L 1,3‐propanediol from glucose when K. pneumoniae S6 was inoculated after 48 h in the culture of Z. rouxii JL2011 by mixed culture of Z. rouxii JL2011 and K. pneumoniae S6 with initial cell ratio of 1:200. In a 7‐L bioreactor, the maximum 1,3‐propanediol production could reach up to 15.2 g/L. Thus, this study presents an effective process for 1,3‐propanediol microbial production from glucose by using mixed culture of Z. rouxii JL2011 and K. pneumoniae S6. This work does not only demonstrate a new way to produce 1,3‐propanediol from a low‐cost feedstock, but may also make a valuable contribution to the development of a cost‐effective fermentation based on renewable resources.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

1,3‐propanediol production from glucose by mixed‐culture fermentation of Zygosacharomyces rouxii and Klebsiella pneumonia

Ma¹,

Shentu²,

Bian³

et al. 2012

Engineering in Life Sciences

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“…The yield and productivity reached 0.40 g g -1 and 0.79 g l -1 h -1 , respectively. Ringel et al isolated two strains of C. butyricum AKR92a and C. butyricum AKR102a [30]. Both strains could tolerate high crude glycerol concentration to 150 g l -1 and produce 80% of the PDO that from pure glycerol under the same concentration.…”

Section: Natural Selectionmentioning

confidence: 99%

“…Klebsiella pneumoniae AJ4 NA 52.59 0.63 2.02 [27] K. pneumoniae TUAC01 NA 66.3 NA 1.0 [28] Citrobacter freundii FMCC-B 294 (VK-19) 170 68.1 0.48 0.79 [29] Clostridium butyricum AKR102a 150 92.9 NA NA [30] Artificial breeding…”

Section: Natural Isolationmentioning

confidence: 99%

Recent developments in the microbial production of 1,3-propanediol

Tang

Liu³

et al. 2013

Biofuels

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1,3-propanediol (PDO) is a valuable chemical monomer to polymerize to polytrimethylene terephthalate with terephthalic acid. Polytrimethylene terephthalate is considered as one kind of prospective new polyester. Production of PDO by microbial fermentation leads to a significantly cost-effective process and decreases nonrenewable resources. This article summarizes the latest developments in the microbial production of PDO, including the selection of high-performance PDO-producing microorganisms, the conversion of crude glycerol derived from biodiesel plants to PDO and different fermentation strategies adopted in the fermentation process. Furthermore, genetic engineering is discussed, a technology that provides a more convenient method to obtain microorganisms with high PDO-producing ability. Microbial production of PDO would directly benefit the environment by reducing the use of fossil fuels and elevating the value of biodiesel production, which has become a hotspot in recent decades.

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An Improved Screening Method for Microorganisms Able to Convert Crude Glycerol to 1,3-Propanediol and to Tolerate High Product Concentrations

Cited by 2 publications

References 0 publications

1,3‐propanediol production from glucose by mixed‐culture fermentation of Zygosacharomyces rouxii and Klebsiella pneumonia

1,3‐propanediol production from glucose by mixed‐culture fermentation of Zygosacharomyces rouxii and Klebsiella pneumonia

Recent developments in the microbial production of 1,3-propanediol

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