An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement

Alt, Volker; Bechert, T.; Steinrücke, Peter; Wagener, Michael; Seidel, Peter; Dingeldein, E.; Domann, Eugen; Schnettler, Reinhard

doi:10.1016/j.biomaterials.2003.10.078

Cited by 873 publications

(587 citation statements)

References 35 publications

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“…However, in this study, neither the 0.6%, nor the 1% silver cement had any effect in the prevention of infection. In spite of the previous promising reports, 24,26 which showed good efficacy against antibiotic-sensitive and -resistant bacteria both in vitro and in vivo, the silver cement scored on no outcome parameter significantly different from the plain cement. On the primary outcome of ''infection/no infection,'' it even showed a 100% infection rate.…”

Section: Discussioncontrasting

confidence: 63%

“…Although the Staphylococcus aureus Wood 46, used in this study, has often been used in infection research, 1,27 it could have had a higher virulence compared to the antibioticsensitive and -resistant coagulase-negative Staphylococci and Staphylococcus aureus used by Alt et al, 24 Finally, small differences in how the surgical procedure was exactly performed in the two studies could be an explanation. Unfortunately, this is difficult to determine, as the procedure performed by Alt et al in their in vivo study is not described in detail.…”

Section: Discussionmentioning

confidence: 88%

“…The cement contained a type of nanoparticulate metallic silver, called NanoSilver. 24,25 This silver has a much higher active surface and the nanoparticulate silver particles form small aggregates. Therefore, the nanoparticulate silver has a much higher porosity compared to commercial silver powder.…”

mentioning

confidence: 99%

“…This silver, which was added to a not commercially available PMMA bone cement, showed good in vitro results against Staphylococcus epidermidis, MRSA, and MRSE, and showed no in vitro cytotoxicity against osteoblasts. 24 In addition, it was also effective in preventing infection with MRSA in an experimental rabbit infection model. 26 For this silver cement, the main antimicrobial activity is claimed to be a surface phenomenon, which prevents the presence of viable bacteria at the implant surface.…”

mentioning

confidence: 99%

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No efficacy of silver bone cement in the prevention of methicillin‐sensitive Staphylococcal infections in a rabbit contaminated implant bed model

Moojen

Charles

Fleer

et al. 2009

Journal Orthopaedic Research

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Data from literature showed that a new type of metallic silver PMMA cement had good results in infection prophylaxis. This study investigated the in vivo efficacy of silver cement in the prevention of methicillin-sensitive Staphylococcal infections, compared to plain and tobramycin-containing cement. In 48 rabbits, 0.6% silver, 1% silver, plain, or tobramycin PMMA cement was injected into the femoral medullary canal after contamination with 10 5 , 10 6 , or 10 7 colony forming units (CFU) Staphylococcus aureus. After 14 days, bone was collected for bacteriology and histopathology. All plain and silver cement rabbits were infected, whereas only two tobra rabbits were infected (p < 0.001). The number of bacteria cultured ( 10 logCFU) from bone adjacent to the cement, was 6.4 AE 0.3 and 6.1 AE 0.3 for the 0.6% and 1% silver rabbits. For the rabbits with plain and tobra cement, this was 6.2 AE 0.2 (p > 0.95) and 0.0 AE 0.0 (p < 0.001), respectively. Two tobra rabbits had a positive culture of a distal bone sample. Histological sections of plain, 0.6%, and 1% silver rabbits all showed signs of infection; these signs were absent in the tobra rabbits. Silver and plain cement were not effective in preventing infection, whereas tobra cement was effective. As silver cement predominantly exhibits an antimicrobial effect at the direct cement surface, this cement seems less useful in situations where there are bacteria present in surrounding tissues, like revision surgery. Whether silver cement has relevance in the prevention of bacterial colonization of cement remains to be determined. ß

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Section: Discussioncontrasting

confidence: 63%

Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

mentioning

confidence: 99%

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No efficacy of silver bone cement in the prevention of methicillin‐sensitive Staphylococcal infections in a rabbit contaminated implant bed model

Moojen

Charles

Fleer

et al. 2009

Journal Orthopaedic Research

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“…AgNPs are utilized in water and air filtration and several medical applications [9]. Although various chemical and biochemical methods are being explored for silver NPs (AgNP) production [10], microbes, plants are also very effective in this process.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Metal Nanoparticles Synthesized From Hibiscus Rosa Sinesis and Their Antibacterial Activity Estimation Against Nine Pathogenic Bacteria

Tyagi

Kumar

Tyagi

2017

Asian J Pharm Clin Res

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Objective: This study demonstrates a simple, cost-effective protocol for biosynthesis of stable silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) from Hibiscus rosa-sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: AgNPs and AuNPs were biosynthesized from H. rosa-sinesis. The antibacterial activities of biosynthesized AgNPs (bio-AgNPs) and biosynthesized AuNPs (bio-AuNPs) were evaluated against 9 pathogenic bacterial species Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Biological synthesized AgNP/AuNPs were characterized by an ultraviolet-visible (UV-Vis) spectrophotometer, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The existence of silver and gold ions in the extracts was conformed (primary confirmation) through UV-Vis and the maximum plasmon peak was observed at 402 and 530 nm of bio-AgNPs and bio-AuNPs, respectively. Bio-AgNPs were obtained in 13.01-28.14 nm size range, whereas bio-AuNPs were in 6.32-18.19 nm size range after analysis of TEM images. The results of FTIR spectra indicate that the bio-AuNPs were bound to amine groups and bio-AgNPs to carboxylate ion groups. The antibacterial activities of AgNP/AuNP, the zone of inhibition significantly increased with the increases of concentrations of bio-AgNPs in all pathogenic bacterial species except in the case of S. epidermidis at 50%, Streptococcus aerogenes and A. hydrophila at 70%, whereas in the case of bio-AuNPs antibacterial activities were displayed only against B. subtilis at 20% and 100% concentration. Conclusion:This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from H. rosa-sinensis leaf extract and insights to their potential applicability as an alternative antibacterial agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria.

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Computational Design and Prediction of Interesting Not-Yet-Synthesized Structures of Inorganic Materials by Using Building Unit Concepts

et al. 2002

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An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement

Cited by 873 publications

References 35 publications

No efficacy of silver bone cement in the prevention of methicillin‐sensitive Staphylococcal infections in a rabbit contaminated implant bed model

No efficacy of silver bone cement in the prevention of methicillin‐sensitive Staphylococcal infections in a rabbit contaminated implant bed model

Comparative Analysis of Metal Nanoparticles Synthesized From Hibiscus Rosa Sinesis and Their Antibacterial Activity Estimation Against Nine Pathogenic Bacteria

Computational Design and Prediction of Interesting Not-Yet-Synthesized Structures of Inorganic Materials by Using Building Unit Concepts

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