An In Vitro Bladder Model for Studying Catheter-Associated Urinary Tract Infection and Associated Analysis of Biofilms

Nzakizwanayo, Jonathan; Pelling, H.; Milo, Scarlet; Jones, Brian V.

doi:10.1007/978-1-4939-9601-8_14

Cited by 16 publications

(18 citation statements)

References 30 publications

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“…Other uropathogenic strains that can be identified with the biofilm associated with the urinary catheter can also be tested. New quantification and microbial analysis methods will meet practical needs regarding the presence of bacteria in the urinary tract [50].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

Vamanu

Dinu

Luntraru³

et al. 2021

Applied Sciences

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Bioactive compounds and phenolic compounds are viable alternatives to antibiotics in recurrent urinary tract infections. This study aimed to use a natural functional product, based on the bioactive compounds’ composition, to inhibit the uropathogenic strains of Escherichia coli. E. coli ATCC 25922 was used to characterize the IVCM (new in vitro catheterization model). As support for reducing bacterial proliferation, the cytotoxicity against a strain of Candida albicans was also determined (over 75% at 1 mg/mL). The results were correlated with the analysis of the distribution of biologically active compounds (trans-ferulic acid-268.44 ± 0.001 mg/100 g extract and an equal quantity of Trans-p-coumaric acid and rosmarinic acid). A pronounced inhibitory effect against the uropathogenic strain E. coli 317 (4 log copy no./mL after 72 h) was determined. The results showed a targeted response to the product for tested bacterial strains. The importance of research resulted from the easy and fast characterization of the functional product with antimicrobial effect against uropathogenic strains of E. coli. This study demonstrated that the proposed in vitro model was a valuable tool for assessing urinary tract infections with E. coli.

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Section: Discussionmentioning

confidence: 99%

In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

Vamanu

Dinu

Luntraru³

et al. 2021

Applied Sciences

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“…In vitro bladder model testing. The efficacy of 2-MA in a clinically representative system was assessed using in vitro bladder models, as previously described 56 . Physiological conditions (including temperature and urine flow rate) were maintained within the bladder models, which were inoculated with P. mirabilis and E. coli at 10 8 colony forming units (CFU)/mL to model a late stage CAUTI.…”

Section: Qm Modelling Of S Pasteurii Urease Active Site An Inherentmentioning

confidence: 99%

“…In vitro bladder models and analysis. Bladder models and artificial urine were prepared according to Nzakizwanayo et al 56 (Tables 2 and 3).…”

Section: Composition Of Stuart's Broth (Sb)mentioning

confidence: 99%

A small-molecular inhibitor against Proteus mirabilis urease to treat catheter-associated urinary tract infections

Milo

Heylen

Glancy

et al. 2021

Sci Rep

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Infection and blockage of indwelling urinary catheters is significant owing to its high incidence rate and severe medical consequences. Bacterial enzymes are employed as targets for small molecular intervention in human bacterial infections. Urease is a metalloenzyme known to play a crucial role in the pathogenesis and virulence of catheter-associated Proteus mirabilis infection. Targeting urease as a therapeutic candidate facilitates the disarming of bacterial virulence without affecting bacterial fitness, thereby limiting the selective pressure placed on the invading population and lowering the rate at which it will acquire resistance. We describe the design, synthesis, and in vitro evaluation of the small molecular enzyme inhibitor 2-mercaptoacetamide (2-MA), which can prevent encrustation and blockage of urinary catheters in a physiologically representative in vitro model of the catheterized urinary tract. 2-MA is a structural analogue of urea, showing promising competitive activity against urease. In silico docking experiments demonstrated 2-MA’s competitive inhibition, whilst further quantum level modelling suggests two possible binding mechanisms.

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“…All of the equipment was assembled to simulate the human urinary bladder system. Some of the selected literature established in vitro bladder models based on the same models or with some modifications [two bladder models (13,15) were modified from the model reported by Abbott et al (21); eight bladder models (16,17,19,30,32,33,35,38) were modified from the model reported by Stickler et al (49); two bladder models (32,39) were modified from the model reported by Gaonkar et al (43); one bladder model (28) was modified from the model reported by Fu et al (36); two bladder models (31,34) was modified from the model reported by Andersen et al (37); one bladder model (45) was modified from the model reported by Stickler et al (55); one bladder model (48) was modified from the model reported by Getliffe et al (59); six bladder models (19,41,(50)(51)(52)(53) were modified from the model reported by Stickler et al (58); one bladder model ( 62) was modified from the model reported by Stickler et al (64); 11 bladder models (63,(65)(66)(67)(68)(69)(70)72,73,…”

Section: Model Construction and Analysismentioning

confidence: 99%

“…Of these, 11 studies(13,(21)(22)(23)44,68,69,72,78,85,86) were combined with stirring or rotating instruments and were set to a certain flow rate (15 µL/min to 50 mL/min). Meanwhile, 18 studies(13,15,16,21,23,24,30,53,57,60,61,68,69,(70)(71)(72)(73)75)…”

mentioning

confidence: 99%

In vitro dynamic bladder models for studying urinary tract infections: a narrative review

Xiong¹,

Xie²,

Liu³

2021

Ann Palliat Med

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Experimental models of the bladder are key to studying the pathogenic mechanism of catheterrelated bacterial biofilm infection. Although numerous studies have reported multiple models, these model designs were heterogeneous. This study aimed to review the status quo and explore the problems associated with in vitro dynamic bladder models for studying urinary tract infections (UTIs). The PubMed and SinoMed databases were searched from their inception to February 2020. Studies regarding in vitro bladder models related to UTIs were reviewed based on a bibliometric evaluation of their basic characteristics and model analysis. A total of 74 papers and 44 bladder models were included in this study. The results were as follows: (I) urine transmission devices: 10 studies applied the gravity effect of culture media, while the others used peristaltic pumps, and 11 of them combined stirring or rotating forces. The flow rates in all studies ranged from 15 µL/min to 50 mL/min. (II) Bladder model: two studies reported on simulating the bladder using plastic bags, while the others reported on glass cylinders or fermenters with a capacity of 200 to 700 mL. E. coli and P. mirabilis were the main bacterial strains. (III) Infection carrier: six studies reported planktonic bacteria as their infection carrier, while 45 studies reported silica gel, rubber, polyurethane, silicone, polytetrafluoroethylene, or perfusion bag. (IV) Infection medium: 25 studies reported the culture medium. Thirty-two studies reported artificial urine, while 17 studies reported human urine. (V) Research analysis: 45 studies investigated the bacterial biofilm formation in the bladder model. Thirty-six studies compared the effects of various drug coatings, diverse material surfaces, or different materials. Only five studies compared distinct bladder models. The included studies' main defects were the single simulation of bladder urodynamics, divers parameter settings, and non-standard experimental modeling. Our analysis showed for the first time that in vitro dynamic bladder models could provide new ideas for exploring the mechanism and prevention of bacterial biofilm infection in urinary implanted biomaterials. Due to the limitations of the included studies, more high-quality studies are needed to verify the conclusions above further.

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An In Vitro Bladder Model for Studying Catheter-Associated Urinary Tract Infection and Associated Analysis of Biofilms

Cited by 16 publications

References 30 publications

In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

A small-molecular inhibitor against Proteus mirabilis urease to treat catheter-associated urinary tract infections

In vitro dynamic bladder models for studying urinary tract infections: a narrative review

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