An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria

Kim, Ryan Jin Young; Kim, Myung Ook; Lee, Ki Sun; Lee, Dong Hoon; Shin, Joo Hee

doi:10.1016/j.archoralbio.2015.07.014

Cited by 55 publications

(49 citation statements)

References 47 publications

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“…In the present work, no antimicrobial activity was observed in the ProRoot MTA when in contact with water or blood in the agar diffusion test. This result is in accordance to previous studies 10 30 although no fluids were used to age the materials in the mentioned studies. However, because of the limitations of this technique these results lack reliability.…”

Section: Discussionsupporting

confidence: 93%

“…The antimicrobial properties of ProRoot MTA have been evaluated against different bacteria including E. faecalis obtaining controversial results. Some authors have reported a limited activity 30 whereas it was effective in other studies 30 31 32 33 . However, this is the first study to evaluate the effect of different fluids on the antimicrobial properties of a MTA-based material.…”

Section: Discussionmentioning

confidence: 98%

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Antimicrobial activity of ProRoot MTA in contact with blood

Farrugia

Baca

Camilleri

et al. 2017

Sci Rep

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Dental materials based on Portland cement, which is used in the construction industry have gained popularity for clinical use due to their hydraulic properties, the interaction with tooth tissue and their antimicrobial properties. The antimicrobial properties are optimal in vitro. However in clinical use contact with blood may affect the antimicrobial properties. This study aims to assess whether antimicrobial properties of the Portland cement-based dental cements such as mineral trioxide aggregate (MTA) are also affected by contact with blood present in clinical situations. ProRoot MTA, a Portland cement-based dental cement was characterized following contact with water, or heparinized blood after 1 day and 7 days aging. The antimicrobial activity under the mentioned conditions was assessed using 3 antimicrobial tests: agar diffusion test, direct contact test and intratubular infection test. MTA in contact with blood was severely discoloured, exhibited an additional phosphorus peak in elemental analysis, no calcium hydroxide peaks and no areas of bacterial inhibition growth in the agar diffusion test were demonstrated. ProRoot MTA showed limited antimicrobial activity, in both the direct contact test and intratubular infection test. When aged in water ProRoot MTA showed higher antimicrobial activity than when aged in blood. Antimicrobial activity reduced significantly after 7 days. Further assessment is required to investigate behaviour in clinical situations.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Antimicrobial activity of ProRoot MTA in contact with blood

Farrugia

Baca

Camilleri

et al. 2017

Sci Rep

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“…P. gingivalis was selected for the present study as it is one of the most frequently detected anaerobic microorganisms in subgingival plaque samples from periodontal-endodontic combined lesions and necrotic pulp [ 6 ]. S. mutans , S. gordonii , S. sobrinus and L. lactis were used as are the most frequently detected microorganisms in cariogenic plaque [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

“…Microorganisms remained after canal treatment will impair periapical healing and will facilitate developing apical lesions [ 5 ]. Porphyromonas gingivalis ( Pg ) is a major etiologic agent not only in the recurrent infections after endodontic treatment [ 6 ], but also in the development and progression of periapical lesions and periodontitis [ 7 ]. Biomaterials design leading to minimize the incidence of persistent or recurrent infections of the root canal system and apical periodontitis would also be desirable [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Modified Polymeric Nanoparticles Exert In Vitro Antimicrobial Activity Against Oral Bacteria

et al. 2018

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Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis, Lactobacillus lactis, Streptoccocus mutans, gordonii and sobrinus were grown and the number of bacteria was determined by optical density. Nanoparticles were suspended in phosphate-buffered saline (PBS) at 10, 1 and 0.1 mg/mL and incubated with 1.0 mL of each bacterial suspension for 3, 12, and 24 h. The bacterial viability was assessed by determining their ability to cleave the tetrazolium salt to a formazan dye. Data were analyzed by ANOVA and Scheffe’s F (p < 0.05). Doxycycline doping efficacy was 70%. A burst liberation effect was produced during the first 7 days. After 21 days, a sustained release above 6 µg/mL, was observed. Calcium and zinc liberation were about 1 and 0.02 µg/mL respectively. The most effective antibacterial material was found to be the Dox-Nanoparticles (60% to 99% reduction) followed by Ca-Nanoparticles or Zn-Nanoparticles (30% to 70% reduction) and finally the non-doped nanoparticles (7% to 35% reduction). P. gingivalis, S. mutans and L. lactis were the most susceptible bacteria, being S. gordonii and S. sobrinus the most resistant to the tested nanoparticles.

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“…All materials tested in our study had the highest antibacterial effects against S. aureus and the lowest against E. faecalis, which is in accordance with results of some previous studies 10,22 . In addition, the MTA based materials may also fail to inhibit the growth of E. faecalis 26 , but inhibited the growth of caries-associated bacteria 27,28 . An increase in inhibition zones exceeding 10%, between 1h and 24h, was observed in the case of CH and MTA + against E. faecalis ATCC 14506, and in the case of CH, MTA + and CS against Rothia dentocariosa.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial effects of new endodontic materials based on calcium silicates

Trišić¹,

Ćetenović

Zdravković

et al. 2019

VOJNOSANIT PREGL

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Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.

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An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria

Cited by 55 publications

References 47 publications

Antimicrobial activity of ProRoot MTA in contact with blood

Antimicrobial activity of ProRoot MTA in contact with blood

Modified Polymeric Nanoparticles Exert In Vitro Antimicrobial Activity Against Oral Bacteria

Antibacterial effects of new endodontic materials based on calcium silicates

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