An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration

Abstract

Currently, wound infection is an important health problem for the public. Wound infection can not only hinder healing but it can also lead to serious complications. Injectable wound dressings with biocompatible and antibacterial properties can promote wound healing during skin infections and reduce antibiotic use. Here, we used glycidyl methacrylate (GMA) to modify ε-polylysine (ε-PL) and γ-poly(glutamic acid) (γ-PGA) to produce ε-polylysine-glycidyl methacrylate (ε-PL-GMA) and γ-poly(glutamic acid)-glycidyl methacrylate (γ-PGA-GMA). Subsequently, ε-PL-GMAand γ-PGA-GMA-based hydrogels were developed through photopolymerization using visible light. The hydrogels were injectable, could rapidly gelatinize, were biocompatible, and showed a wide spectrum of antibacterial activity. The hydrogels also promoted wound healing. The results show that these hydrogels inhibit bacterial infection and shorten the wound healing time of skin defects in Staphylococcus aureus models. This demonstrates that the hydrogels hold potential for clinical antimicrobial and wound healing therapy.

Development of next-generation antimicrobial hydrogel dressing to combat burn wound infection

“…Using the partial-thickness burn injury model, followed by infection with a clinical strain of a multidrug-resistant gram-negative A. baumannii, Khan et al, demonstrate the ability of the newly developed EPL-hydrogel to significantly reduce in-vivo biofilms in burn wounds (Fig 2) which is most likely attributed to the action of both EPL and function of reactive oxygen species (ROS) and hydrogen peroxide (H2O2) byproducts of catechol oxidation. Indeed, this agrees with previous studies which have also described the use of injectable EPL nanocomposite hydrogel to combat infection and promote tissue healing and regeneration in-vivo [15] however exact mechanisms of actions in burn wounds are yet to be determined. The tunable functionalities of the EPL-catechol biomaterial make it a promising product for future investigation in biomedical applications.…”

The Photoinitiator Lithium Phenyl (2,4,6-Trimethylbenzoyl) Phosphinate with Exposure to 405 nm Light Is Cytotoxic to Mammalian Cells but Not Mutagenic in Bacterial Reverse Mutation Assays

“…LAP in cell culture medium is exposed to the in vitro equivalent of the apical membrane of collecting duct cells. Additionally, the use of this cell model would expand the scope to technologies that could directly contact native tissue, such as tissue adhesives, injectable wound dressings, or hand-held extrusion printers for bioprinting during surgery [ 26 , 27 , 28 ]. Therefore, the objectives of this study were to: (1) characterize light exposure conditions typical of bioprinters, using LEDs as a light source; (2) assess whether LAP with concurrent light exposure enhances cytotoxicity in confluent M-1 cells; and (3) determine if exaggerated exposure conditions associated with high cytotoxicity are mutagenic using bacterial reverse mutation assays.…”

Recent Insights into Emerging Coronavirus: SARS-CoV-2

“… 15 In a genome-wide association study, SARS-CoV-2 was found to have a similarity of 79.5% in genomic sequence to the SARS coronavirus and to be 96% homologous to that of a bat-derived coronavirus, suggesting that bats are the main source of transmission of SARS-CoV-2. 16 Aside from bats, pangolins have been regarded as a possible source of SARS-CoV-2 transmission as well. Lam et al discovered that pangolin-derived coronaviruses were closely associated with two sublineages of SARS-CoV-2-related coronaviruses through metagenomic sequencing.…”