An integrative overview of the molecular and physiological responses of sugarcane under drought conditions

Vital, Camilo Elber; Giordano, Andrea; Soares, Eduardo de Almeida; Williams, Thomas Christopher Rhys; Mesquita, Rosilene Oliveira; Vidigal, Pedro Marcus Pereira; Lopes, Amanda de Santana; Pacheco, Túlio Gomes; Rogalski, Marcelo; Ramos, Humberto Josué de Oliveira; Loureiro, Marcelo Ehlers

doi:10.1007/s11103-017-0611-y

Cited by 34 publications

(20 citation statements)

References 70 publications

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“…acetyl-CoA, propionyl-CoA, and acetoacetate, are potential energy sources for plants (Joshi et al, 2010). The global increase of numerous amino acids likely depicts increased degradation and synthesis of proteins (Vital et al, 2017). Similar observation was reported in crop cultivars, and shown to be linked to impact of osmotic stress on the proteasome (Vital et al, 2017).…”

Section: Early Signs Of Stress Responses In Elodea Nuttalli Treated Wmentioning

confidence: 66%

Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii

Cosio

Renault

2020

Environmental Pollution

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Macrophytes are known to bioaccumulate metals, but a thorough understanding of tolerance strategies and molecular impact of metals in aquatic plants is still lacking. The present study aimed to compare Hg and Cd effects in a representative macrophyte, Elodea nuttallii using physiological endpoints and metabolite profiles in shoots and cytosol. Exposure 24h to methyl-Hg (23 ng•L-1), inorganic Hg (70 ng•L-1) and Cd (281 µg•L-1) did not affect photosynthesis, or antioxidant enzymes despite the significant accumulation of metals, confirming a sublethal stress level. In shoots, Cd resulted in a higher level of regulation of metabolites than MeHg, while MeHg resulted in the largest number of regulated metabolites and IHg treatment regulated no metabolites significantly. In cytosol, Cd regulated more metabolites than IHg and only arginine, histidine and mannose were reduced by MeHg exposure. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of data suggested that exposure to MeHg resulted in biochemical changes including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism, nitrogen metabolism, arginine and proline metabolism, cyanoamino acid metabolism, while the treatment of Cd stress caused significant variations in aminoacyl-tRNA biosynthesis and branched-chain amino acids pathways. Data supports an impact of MeHg on N homeostasis, while Cd resulted in an osmotic stress-like pattern and IHg had a low impact. Marked differences in the responses to MeHg and IHg exposure were evidenced, supporting different molecular toxicity pathways and main impact of MeHg on non-soluble compartment, while main impact of IHg was on soluble compartment. Metabolomics was used for the first time in this species and proved to be very useful to confirm and complement recent knowledge gained by transcriptomics and proteomics, highlighting the high interest of multi-omics approaches to identify early impact of environmental pollution.

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Section: Early Signs Of Stress Responses In Elodea Nuttalli Treated Wmentioning

confidence: 66%

Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii

Cosio

Renault

2020

Environmental Pollution

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“…These studies bring a maximum of 25 proteins differentially accumulated which are mainly related to photosynthesis and homeostasis regulation. Recently, integrated transcriptomics, proteomics, and metabolomics data characterized the molecular responses of sugarcane leaves to drought stress . Although many changes in genes related to photosynthesis, carbohydrate, and amino acid metabolism, the proteome and transcriptome analyses were not always overlapped, which in part could be explained by the utilization of 2D gels.…”

Section: Introductionmentioning

confidence: 99%

Label‐Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

et al. 2019

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Drought is considered the major abiotic stress limiting crop productivity. This study seeks to identify proteins involved in the drought response in sugarcane stems submitted to drought stress. The integration of nuclei enrichment sample preparation with the shotgun proteomic approach results in great coverage of the sugarcane stem proteome with 5381 protein groups identified. A total of 1204 differentially accumulated proteins are detected in response to drought, among which 586 and 618 are increased and reduced in abundance, respectively. A total of 115 exclusive proteins are detected, being 41 exclusives of drought-stressed plants and 74 exclusives of control plants. In the control plants, most of these proteins are related to cell wall metabolism, indicating that drought affects negatively the cell wall metabolism. Also, 37 transcription factors (TFs) are identified, which are low abundant nuclear proteins and are differentially accumulated in response to drought stress. These TFs are associated to protein domains such as leucine-rich (bZIP), C2H2, NAC, C3H, LIM, Myb-related, heat shock factor (HSF) and auxin response factor (ARF). Increased abundance of chromatin remodeling and RNA processing proteins are also observed. It is suggested that these variations result from an imbalance of protein synthesis and degradation processes induced by drought.

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“…The detection of the phosphorylated proteins was carried out by staining the 2DE gels using a Pro-Q ® Diamond Phosphoprotein Gel Stain kit (Invitrogen, Calrsbad, USA), according to the protocol described by Agrawal and Thelen [22]. The gels were then scanned with a FLA 5100 laser scanner and the images were analyzed as described by Vital [23].…”

Section: Gelsmentioning

confidence: 99%

Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

2019

Crop Breed Genet Genom.

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Drought is the major abiotic stress which contributes to the reduction in the productivity of soybeans in Brazil, especially in the savanna regions. Two parental genotypes contrasting in drought tolerance (Embrapa 48tolerant and BR 16-sensitive) were used to study the molecular mechanism underlying this process in soybeans. The hydric potential of the BR 16 reached values of −1.0 and −1.5 MPa, three days before the Embrapa 48, confirming the contrasting drought tolerance. The proteomic, phosphoproteomic and metabolomic profiles were evaluated to detect the metabolic pathways, which were affected by the drought stress. An integrative overview showed that the tolerant plants maintain cell homeostasis and the photosynthetic metabolism was unchanged under the stress condition in contrast to the sensitive genotype that showed several dysregulated pathways. These findings were confirmed by the protein expression and protein regulation by phosphorylation. Furthermore, just small deviations in the metabolic pathways were observed for drought-tolerant plants in comparison to the sensitive genotype. Complex post-translational modification patterns by phosphorylation were detected in some key enzymes, such as carbonic

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An integrative overview of the molecular and physiological responses of sugarcane under drought conditions

Cited by 34 publications

References 70 publications

Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii

Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii

Label‐Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

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