An optical biosensor-based immunoassay for the determination of bovine serum albumin in milk and milk products

Indyk, Harvey E.; Gill, Brendon D.; Woollard, David C

doi:10.1016/j.idairyj.2015.02.011

Cited by 21 publications

(8 citation statements)

References 45 publications

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“…The proteotypic peptide LVNELTEFAK were used to quantify BSA in raw bovine milk. On the basis of the proteotypic peptide, the concentration of bovine serum albumin in tenfold-diluted raw bovine milk was 16.93 ± 0.80 mg/kg, similar to the BSA contents reported by Indyk et al 26 Method validation of proteotypic peptides in BSA quantification was reported as follows.…”

Section: Resultssupporting

confidence: 84%

Peptide Selection for Accurate Targeted Protein Quantification via a Dimethylation High-Resolution Mass Spectrum Strategy with a Peptide Release Kinetic Model

et al. 2020

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A crucial step in accurate targeted protein quantification using targeted proteomics is to determine optimal proteotypic peptides representing targeted proteins. In this study, a workflow of peptide selection to determine proteotypic peptides using a dimethylation high-resolution mass spectrum strategy with a peptide release kinetic model was investigated and applied in peptide selection of bovine serum albumin. After specificity, digestibility, recovery, and stability evaluation of tryptic peptides in bovine serum albumin, the optimal proteotypic peptide was selected as LVNELTEFAK. The quantification method using LVNELTEFAK gave a linear range of 1−100 ppm with the coefficient greater than 0.9990, and the detection limit of bovine serum albumin in milk was 0.78 mg/kg. Compared with the proteotypic peptides selected by Skyline, the method showed a better performance in method validation. The workflow exhibited high comprehensiveness and efficiency in peptide selection, facilitating accurate targeted protein quantification in the food matrix, which lack protein standards.

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Section: Resultssupporting

confidence: 84%

Peptide Selection for Accurate Targeted Protein Quantification via a Dimethylation High-Resolution Mass Spectrum Strategy with a Peptide Release Kinetic Model

et al. 2020

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“…Thus, a receptor such as an antibody could be immobilized on the surface of the metal. Compared with other techniques, SPR technology permits a label-free and real-time analysis [9,10]. Recently, SPR was widely used in the study of interactions of various biological molecules, ranging from proteins, oligonucleotides and lipids, to small particles [11].…”

Section: Introductionmentioning

confidence: 99%

Kinetic and thermodynamic analysis of ultra-high pressure and heat-induced denaturation of bovine serum albumin by surface plasmon resonance

Wang¹,

Zhu²,

Chen³

et al. 2017

Trop. J. Pharm Res

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“…The combination of the high selectivity of SMIP based on the strong recognition effect between SMIP and BSA and the highly sensitive CL determination method made the proposed biosensor perform excellently in the determination of BSA. Based on this, future work will 1.4 Â 10 À5 Optical biosensor 3 1.0 Â 10 À5 -1.0 Â 10 À3 2.5 Â 10 À6 Flow injection analysis 24 0.0-28.0 0.76 focus on the preparation of synthetic receptor materials as SMIP elements with higher adsorption capacity and selectivity for the fabrication of biomimetic CL biosensors.…”

Section: Discussionmentioning

confidence: 99%

“…2 The level of BSA in bovine milk is used as a marker of the health of the mammary gland and of milk quality. 3 Analytical approaches for the determination of bovine serum albumin that have been proposed include chemometrics, 2 optical biosensors, 3 etc. Cheap, convenient and sensitive chemiluminescence (CL) 4 biosensors for selective determination of BSA have been intentionally developed, but specic molecular recognition ability is required.…”

Section: Introductionmentioning

confidence: 99%

β-Cyclodextrin/chitosan–magnetic graphene oxide–surface molecularly imprinted polymer nanocomplex coupled with chemiluminescence biosensing of bovine serum albumin

Duan

Wang

et al. 2015

RSC Adv.

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8In this report, a sensitive and selective chemiluminescence (CL) biosensor for 9 bovine serum albumin (BSA) coupled with surface molecularly imprinted polymer 10 nanocomplex using β-cyclodextrin/chitosan-magnetic graphene oxide as backbone 11 material (β-CD/Cs-MGO-SMIP) was promoted. Then, the material β-CD/Cs-MGO in 12 which β-cyclodextrin, chitosan and graphene oxide was used to provide 13 multi-imprinting sites and large surface area was characterized by SEM, XRD and 14 FTIR, and then it was found that β-CD/Cs-MGO-SMIP followed Langmuir isotherm 15 equation and pseudo-second order sorption kinetics when binding the template. It 16 demonstrated fast mass transfer, promoted rate of removal of the biomacromolecule 17 and excellent recognition and adsorption ability for the imprinting cavities situated at 18 the surface of the β-CD/Cs-MGO, which enabled easy access to BSA. Subsequently, a 19high sensitive CL biosensor to BSA based on the strong recognition effect between 20 β-CD/Cs-MGO-SMIP and BSA which decided the high selectivity has been proposed 21 and the proposed biosensor could be assay in the range of 5.0 2 mg/mL with a detection limit of 1.1 × 10 -7 mg/mL. The obtained recoveries were 23 between 94% and 106% when determining samples. 24 Keywords: chemiluminescence biosensor; bovine serum albumin; magnetic graphene oxide; 25 β-cyclodextrin; chitosan; surface molecularly imprinted polymer 26 42 materials that could improve the selectivity and adsorbing capacity to target 43 biomacromolecule was particularly important [6]. Recently, chitosan (Cs) has attached considerable attention [7] as one of the most 45 promising materials due to its biodegradability, biocompatibility and non-toxicity [8]. 46 With a natural mucopolysaccharide with similar structural characteristics to cellulose, 47 Cs has been applied in preparation of molecularly imprinted resin [9], 48 integrated-optical sensors [10] and porous membrane [11]. On account of the 49 abundant hydroxyl and amino group, when preparing surface molecular imprinting 50 polymer (SMIP), Cs was full of the advantages of multiple imprinting sites which 51 would accelerate the imprinting process, improve the selectivity and adsorbing 52 capacity [12].53 β-cyclodextrin (β-CD), with a lipophilic inner cavity with hydrophilic outer 54 surfaces which was able to interact with a large variety of guest molecules forming 55 non-covalent inclusion complexes [13], had already demonstrated its potential in 56 separation and analytical application since it could bind angstrom-sized guests 57 through apolar interaction in protic media [14]. As a supramolecular host compound, 58 β-CD was willing to be chosen as functional monomer to prepare MIP to achieve high 59 selectivity and great adsorption capacity [15]. 60 In current century, as a fascinating new carbon materials with honeycomb and 61 one-atom-thick structure, graphene oxiede (GO) has attracted worldwide attention 62 [16]. Due to its large specific surface area, good biocompatibility and chemical 63 stability, GO was us...

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An optical biosensor-based immunoassay for the determination of bovine serum albumin in milk and milk products

Cited by 21 publications

References 45 publications

Peptide Selection for Accurate Targeted Protein Quantification via a Dimethylation High-Resolution Mass Spectrum Strategy with a Peptide Release Kinetic Model

Peptide Selection for Accurate Targeted Protein Quantification via a Dimethylation High-Resolution Mass Spectrum Strategy with a Peptide Release Kinetic Model

Kinetic and thermodynamic analysis of ultra-high pressure and heat-induced denaturation of bovine serum albumin by surface plasmon resonance

β-Cyclodextrin/chitosan–magnetic graphene oxide–surface molecularly imprinted polymer nanocomplex coupled with chemiluminescence biosensing of bovine serum albumin

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