An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Turcanu, Mihnea V.; Cochran, Sandy; Moldovan, Alexandru; Vlatakis, Stavros; Vllasaliu, Driton; Thanou, Maya; Näthke, Inke

doi:10.1109/ius46767.2020.9251401

Cited by 3 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is because as soon as a sufficiently large pore is produced in the monolayer, an electric path is created between the two electrodes, reflected by low TEER values. Continuing to insonate might increase the size of the formed hole, but because the electrons already have a free passage to move between the two electrodes, TEER values do not reflect this effect proportionally (Turcanu et al, 2020). However, a larger pore enables more passive diffusion of FD4 from the apical to the basolateral chamber.…”

Section: Discussionmentioning

confidence: 99%

“…INTRODUCTION Ultrasound (US) and microbubbles (MBs) can promote the delivery of therapeutic compounds across epithelial layers (Stewart et al, 2018, Turcanu et al, 2020, which can be used to mark tissue in vivo (Turcanu et al, 2018;Stewart et al, 2021;). However, in vitro experiments to study this effect are often hindered by the inability to deliver buoyant contrast agents in proximity to cell monolayers to adequately control the decrease in barrier function whilst insonating a sufficiently large tissue area.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Manipulating the Barrier Function of a Cell Monolayer Using a High-power Miniature Ultrasonic Transducer

Turcanu

Moldovan

Thanou

et al. 2021

2021 IEEE International Ultrasonics Symposium (IUS)

Self Cite

View full text Add to dashboard Cite

Ultrasound (US) and cavitation agents such as microbubbles (MBs) have been demonstrated to decrease the barrier function of endothelial and epithelial layers. However, in vitro experiments that study this effect are often hindered by the inability to deliver buoyant contrast agents in proximity to cell monolayers in order to adequately control the decrease in barrier function whilst insonating a sufficiently large tissue area. We have addressed this by adapting a cell culture system and fabricating a bespoke high-power miniature unfocused US transducer. The setup was used to control the drop in barrier function and to determine how varying the mechanical index (MI) and the duty cycle affected the barrier function. It was found that buffer solution alone and buffer + MBs did not decrease the transepithelial electrical resistance (TEER) of the cell monolayer. Buffer + US decreased the TEER by ~40%, with 10% TEER recovery 9 min after switching US off. Buffer + MBs + US decreased the TEER by 80%, with little or no recovery following treatment. In the presence of MBs, the barrier function was decreased by a duty cycle = [1% -50%] and by an MI = [0.25 -0.5], without any recovery following treatment. Detectable amounts of fluorescent dextran were delivered across the Caco-2 monolayer only by a combination of US + MBs. These results suggest that our adapted setup and custom-built miniature transducer permits control of the decrease in barrier function for further therapeutic investigations.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Manipulating the Barrier Function of a Cell Monolayer Using a High-power Miniature Ultrasonic Transducer

Turcanu

Moldovan

Thanou

et al. 2021

2021 IEEE International Ultrasonics Symposium (IUS)

Self Cite

View full text Add to dashboard Cite

show abstract

“…Both treatments decreased the barrier to half from their controls. A 13-fold increase in insulin permeation was observed as compared to US+NDs using US+MBs …”

Section: Interaction Of Ultrasound With Biological Systems: Physical ...mentioning

confidence: 93%

“…A 13-fold increase in insulin permeation was observed as compared to US+NDs using US+MBs. 97 Intranasal Applications. Focused ultrasound-mediated intranasal (FUSIN) brain drug delivery may be a promising approach for drug delivery to the brain.…”

Section: Interaction Of Ultrasound Withmentioning

confidence: 99%

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Bansal

Jha

Bhatia

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

The role of ultrasound in medicine and biological sciences is expanding rapidly beyond its use in conventional diagnostic imaging. Numerous studies have reported the effects of ultrasound on cellular and tissue physiology. Advances in instrumentation and electronics have enabled successful in vivo applications of therapeutic ultrasound. Despite path breaking advances in understanding the biophysical and biological mechanisms at both microscopic and macroscopic scales, there remain substantial gaps. With the progression of research in this area, it is important to take stock of the current understanding of the field and to highlight important areas for future work. We present herein key developments in the biological applications of ultrasound especially in the context of nanoparticle delivery, drug delivery, and regenerative medicine. We conclude with a brief perspective on the current promise, limitations, and future directions for interfacing ultrasound technology with biological systems, which could provide guidance for future investigations in this interdisciplinary area.

show abstract

Emerging strategies of engineering retinal organoids and organoid-on-a-chip in modeling intraocular drug delivery: Current progress and future perspectives

Yin

Leng

et al. 2023

Advanced Drug Delivery Reviews

View full text Add to dashboard Cite

An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Cited by 3 publications

References 5 publications

Manipulating the Barrier Function of a Cell Monolayer Using a High-power Miniature Ultrasonic Transducer

Manipulating the Barrier Function of a Cell Monolayer Using a High-power Miniature Ultrasonic Transducer

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Emerging strategies of engineering retinal organoids and organoid-on-a-chip in modeling intraocular drug delivery: Current progress and future perspectives

Contact Info

Product

Resources

About