An Overview of Plant Volatile Metabolomics, Sample Treatment and Reporting Considerations with Emphasis on Mechanical Damage and Biological Control of Weeds

Abstract

It is recommended that in addition to a non-damaged treatment, plants be damaged prior to collecting volatiles to provide the greatest diversity of odours. For the model system provided, optimal volatile emission occurred when the leaf was punctured with a needle. Results stored in a database should include basic environmental conditions or treatments.

Duration of emission of volatile organic compounds from mechanically damaged plant leaves

“…Any study to quantitatively measure VOCs emitted by plants should take into consideration the large difference between those with damaged and undamaged leaves (Smith and Beck, 2013;Beck et al, 2014). However, our results suggest that once plants have been damaged, the VOC profiles are stable for at least 2 days.…”

Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application

“…[11] Metabolomics has been used extensively in various fields, such as in the pharmaceutical industry, plant mechanism, and specific biomarker discovery for clinical diagnosis. [12][13][14] The application of metabolomics in cell culture systems has recently started to attract much interest. Metabolomic studies on the cultured cells, [15] especially in a single cell, [16] have been increasingly reported.…”

In‐field volatile analysis employing a hand‐held portable GC‐MS: emission profiles differentiate damaged and undamaged yellow starthistle flower heads

“…Recent technology has improved the ability to collect and analyse plant‐emitted odours, and help understand their influential role with other plants, insects and microbes (Beck et al ., ). In particular there have been efforts towards development of portable gas chromatography‐mass spectrometry (GC‐MS) systems for the separation and detection of complex volatile bouquets produced by plant–plant, plant–insect or plant–microbe interactions (Miresmailli et al ., ; Schott et al ., ; Aksenov et al ., ).…”