An overview of the microbial -amylase family

Reddy, N. S.; Nimmagadda, Annapoorna; Rao, K. R. S. Sambasiva

doi:10.5897/ajb2003.000-1119

Cited by 113 publications

(13 citation statements)

References 34 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Given their multiple critical properties, including the use of mild reaction conditions, low toxicity, good selectivity and lack of secondary reactions, they are used more frquenty than other traditional chemical processes 1,2 . α-Amylase (1,4-D-glucan glucanohydrolase) catalyses the hydrolysis of the α-D-(1,4) glycosidic linkages of glycogen, starch, and other oligosaccharides, and is found in microorganisms, mammalian tissues and plants 3,4 . These enzymes play important roles in industry and are used many fields, including paper, textiles, food and baking, and others 5,6 .…”

Section: Introductionmentioning

confidence: 99%

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Al-Najada

Almulaiky

Aldhahri

et al. 2019

Sci Rep

View full text Add to dashboard Cite

In this study, amidrazone acrylic fabric was applied as an immobilising support for α-amylase. The immobilised α-amylase was characterised by Fourier transform infrared spectroscopy and scanning electron microscopy. Furthermore, the optimum conditions for immobilisation efficiency, immobilisation time, reusability, kinetic parameters and pH, for the immobilisation process were examined. The study demonstrated that with 4% cyanuric chloride, and a pH of 7.0, the highest immobilization efficiency of 81% was obtained. Around 65% of the initial activity was maintained after storage at 4 °C for 8 weeks. The immobilised enzyme retained 53% of its original activity after being reused 15 times and exhibited improved stability compared with the free enzyme in relation to heavy metal ions, pH, temperature and inhibitors. The immobilised enzyme presented kinetic parameters of 2.6 mg starch and 0.65 µmol maltose/mL for K m and V max respectively, compared with 3.7 mg starch and 0.83 µmol maltose/ mL for the free enzyme. The improvements in the enzyme’s catalytic properties, stability and reusability obtained from immobilisation make amidrazone acrylic fabric support a good promising candidate for bio-industrial applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Al-Najada

Almulaiky

Aldhahri

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A number of carbohydrates noticeably improved bacterial growth, but did not influence bacteriocin production. Most likely, the microorganism is capable of producing a sufficient amount of carbohydrates, since it synthesizes α-amylases and other enzymes, which degrade large sugar polymers into monomers (Reddy et al 2003;Gangadharan et al 2006;Sutyak et al 2008). In addition to carbohydrates, compounds inhibiting bacterial growth, such as ammonium citrate and sodium acetate, were also eliminated.…”

Section: Discussionmentioning

confidence: 98%

“…Several enzymes produced by Bacillus spp. are used in brewing and distilling industries (Reddy et al 2003;Gangadharan et al 2006). In addition, α-amylases have been used in clinical, medical, and analytical chemistries and are safe for human consumption (Reddy et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Subtilosin A production by Bacillus subtilis KATMIRA1933 and colony morphology are influenced by the growth medium

Nikiforova

Klykov²,

Volski

et al. 2015

Ann Microbiol

View full text Add to dashboard Cite

Bacillus subtilis KATMIRA1933, cultured in modified MRS (de Man, Rogosa, and Sharpe) broth without peptone (animal-free [AF]-MRS), produced subtilosin A at levels similar to, or even higher, than when cultured in MRS broth with peptone. AF-MRS medium contained 2.5 % (w/v) Martone or 2.5 % (w/v) cottonseed hydrolysate instead of peptone and 0.25 % (w/v) bacteriological grade yeast extract instead of normal yeast extract. An increase in cell numbers, accompanied by an increase in subtilosin activity, was recorded when cells were grown in AF-MRS supplemented with 0.4 % (w/v) K 2 HPO 4 and 0.02 % (w/v) MgSO 4 . Subtilosin production increased from 30 arbitrary units (AU) mL −1 in a static culture to 320 AU mL −1 when cells were agitated on an orbital shaker at 300 rpm. A further increase in subtilosin production, from 150 AU mL −1 to 240 AU mL −1 , was recorded when cells were cultured in AF-MRS supplemented with 2.0 % (w/v) amylopectin or 2.0 % (w/v) maltodextrin. Slightly higher cell densities were recorded in the presence of maltodextrin. Two colony types, one with a flat (Btypical^), sprawling morphology and the other elevated (Batypical^), were isolated from cells grown in AF-MRS broth. Higher subtilosin levels (213 AU mL −1 ) were recorded from cells grown in AF-MRS broth supplemented with MgSO 4 as compared to cells grown in the absence of MgSO 4 (150 AU mL −1 ).

show abstract

“…The habitat of amyllytic bacteria can be fresh water such as lakes, rivers and ponds. The widely known genus of amyllytic bacteria is Bacillus, Bacterriods, Lactobacillus, Clostridium, Micrococcus, Thermus, and Actinomycetes (Reddy, Nimmagadda, & Rao, 2003).…”

Section: Characterization Of Microbial Morphology From Food and Beverage Samplesmentioning

confidence: 99%

Isolation and Characterization of Amylase-Producing Microbes From the Digestive Tract of Tilapia

et al. 2021

View full text Add to dashboard Cite

Enzymes are natural products that play an important role in various industrial applications, from food processing to chemical products. One of the most widely used enzymes is amylase. Amylase is an enzyme that plays a role in starch hydrolysis. Amylolytic bacteria can be isolated in the digestive tract of fish. This is most bacteria that occupy the digestive tract have high activity. This study aims to obtain a potential isolate producing amylase bacteria whose activity can be determined through morphological characterization and activity test of enzyme-producing bacteria. The results obtained from this study were ten isolates with the potential to produce amylase enzymes, which were marked with a positive test after the addition of iodine.

show abstract

An overview of the microbial -amylase family

Cited by 113 publications

References 34 publications

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Subtilosin A production by Bacillus subtilis KATMIRA1933 and colony morphology are influenced by the growth medium

Isolation and Characterization of Amylase-Producing Microbes From the Digestive Tract of Tilapia

Contact Info

Product

Resources

About