An SPRi-based biosensor pilot study: Analysis of multiple circulating extracellular vesicles and hippocampal volume in Alzheimer’s disease

Picciolini, Silvia; Gualerzi, Alice; Carlomagno, Cristiano; Cabinio, Monia; Sorrentino, Stefano; Baglio, Francesca; Bedoni, Marzia

doi:10.1016/j.jpba.2020.113649

Cited by 25 publications

(21 citation statements)

References 27 publications

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“…The main spectral differences between the two groups are depicted by blue Raman spectra that is obtained by subtraction between PD-derived EVs Raman spectra and healthy control group EVs Raman spectra (adapted with permission from [ 248 ]). (D) Characterization of brain-derived EVs tau oligomers by AFM; (D1) AFM images of EVs associated tau oligomers isolated from AD and prodromal AD (pAD) brains; (D2) graph shows size distribution of Aβ oligomers (adapted with permission from [ 251 ]).…”

Section: Applicationsmentioning

confidence: 99%

Recent advances in optical label-free characterization of extracellular vesicles

et al. 2022

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Extracellular vesicles (EVs) are complex biological nanoparticles endogenously secreted by all eukaryotic cells. EVs carry a specific molecular cargo of proteins, lipids, and nucleic acids derived from cells of origin and play a significant role in the physiology and pathology of cells, organs, and organisms. Upon release, they may be found in different body fluids that can be easily accessed via noninvasive methodologies. Due to the unique information encoded in their molecular cargo, they may reflect the state of the parent cell and therefore EVs are recognized as a rich source of biomarkers for early diagnostics involving liquid biopsy. However, body fluids contain a mixture of EVs released by different types of healthy and diseased cells, making the detection of the EVs of interest very challenging. Recent research efforts have been focused on the detection and characterization of diagnostically relevant subpopulations of EVs, with emphasis on label-free methods that simplify sample preparation and are free of interfering signals. Therefore, in this paper, we review the recent progress of the label-free optical methods employed for the detection, counting, and morphological and chemical characterization of EVs. We will first briefly discuss the biology and functions of EVs, and then introduce different optical label-free techniques for rapid, precise, and nondestructive characterization of EVs such as nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy, surface plasmon resonance spectroscopy, Raman spectroscopy, and SERS spectroscopy. In the end, we will discuss their applications in the detection of neurodegenerative diseases and cancer and provide an outlook on the future impact and challenges of these technologies to the field of liquid biopsy via EVs.

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Section: Applicationsmentioning

confidence: 99%

Recent advances in optical label-free characterization of extracellular vesicles

et al. 2022

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“…(e) Secondary structure prediction of ex-50 aptamer and its shortened version (ex-50.T). In the ELONA-based detection system, plates are coated with EVs and incubated with biotinylated aptamers; signals are detected with streptavidin-HRP [33].…”

Section: Advances In Ev Isolation and Detectionmentioning

confidence: 99%

“…In the search for a reliable, easy and specific method to distinguish cancer cell-derived EVs, nucleic-acid aptamers have been proposed as a promising class of molecules that serve as high-affinity ligands of disease-associated proteins, despite possible non-specific binding risks. For example, the SELEX strategy was developed and validated to isolate aptamers that are specific for EVs [33]. The successful application of the exo-SELEX platform allowed the optimization of a high-affinity aptamer for breast cancer-derived EVs to be used as both an early diagnostic and a prognostic biomarker, but also as a therapeutic target.…”

Section: Advances In Ev Characterization and Biomarker Discoverymentioning

confidence: 99%

“…The successful application of the exo-SELEX platform allowed the optimization of a high-affinity aptamer for breast cancer-derived EVs to be used as both an early diagnostic and a prognostic biomarker, but also as a therapeutic target. Indeed, it was found that the selected sequence (aptamer ex-50.T) inhibited the exosome's cellular uptake, thus antagonizing cancer exosome-induced cell migration (OD14.07) [33]. Specific aptamers were also used for the functionalization of a platform based on metal organic framework, proposed and tested to isolate EVs from breast cancer cell lines with a specific membrane protein.…”

Section: Advances In Ev Characterization and Biomarker Discoverymentioning

confidence: 99%

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Advances in the Field of Micro- and Nanotechnologies Applied to Extracellular Vesicle Research: Take-Home Message from ISEV2021

et al. 2021

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Extracellular Vesicles (EVs) are naturally secreted nanoparticles with a plethora of functions in the human body and remarkable potential as diagnostic and therapeutic tools. Starting from their discovery, EV nanoscale dimensions have hampered and slowed new discoveries in the field, sometimes generating confusion and controversies among experts. Microtechnological and especially nanotechnological advances have sped up biomedical research dealing with EVs, but efforts are needed to further clarify doubts and knowledge gaps. In the present review, we summarize some of the most interesting data presented in the Annual Meeting of the International Society for Extracellular Vesicles (ISEV), ISEV2021, to stimulate discussion and to share knowledge with experts from all fields of research. Indeed, EV research requires a multidisciplinary knowledge exchange and effort. EVs have demonstrated their importance and significant biological role; still, further technological achievements are crucial to avoid artifacts and misleading conclusions in order to enable outstanding discoveries.

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“…Some authors studied the link between EVs and Alzheimer’s disease by comparing EVs values with hippocampal volume as an indicator of neuronal injury. Picciolini et al found a partial correlation between a specific neural EV population (microglia/macrophages—IB4, CD11b) identified by surface plasmon resonance imaging (SPRi) technology and total hippocampal volume detected by MRI analysis [ 93 ]. In particular, the SPRi intensity CD11b/IB4 ratio could be considered as a potential new biomarker of severity, progression, and treatment response in patients with Alzheimer’s disease.…”

Section: Neuroradiology and Evsmentioning

confidence: 99%

Diagnostic Impact of Radiological Findings and Extracellular Vesicles: Are We Close to Radiovesicolomics?

Serafini

Lanuti

Pizzi

et al. 2021

Biology

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Currently, several pathologies have corresponding and specific diagnostic and therapeutic branches of interest focused on early and correct detection, as well as the best therapeutic approach. Radiology never ceases to develop newer technologies in order to give patients a clear, safe, early, and precise diagnosis; furthermore, in the last few years diagnostic imaging panoramas have been extended to the field of artificial intelligence (AI) and machine learning. On the other hand, clinical and laboratory tests, like flow cytometry and the techniques found in the “omics” sciences, aim to detect microscopic elements, like extracellular vesicles, with the highest specificity and sensibility for disease detection. If these scientific branches started to cooperate, playing a conjugated role in pathology diagnosis, what could be the results? Our review seeks to give a quick overview of recent state of the art research which investigates correlations between extracellular vesicles and the known radiological features useful for diagnosis.

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An SPRi-based biosensor pilot study: Analysis of multiple circulating extracellular vesicles and hippocampal volume in Alzheimer’s disease

Cited by 25 publications

References 27 publications

Recent advances in optical label-free characterization of extracellular vesicles

Recent advances in optical label-free characterization of extracellular vesicles

Advances in the Field of Micro- and Nanotechnologies Applied to Extracellular Vesicle Research: Take-Home Message from ISEV2021

Diagnostic Impact of Radiological Findings and Extracellular Vesicles: Are We Close to Radiovesicolomics?

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