Anaerobic ammonium oxidation (anammox) irreversibly inhibited by methanol

Isaka, Kazuichi; Suwa, Yuichi; Kimura, Yuya; Yamagishi, Takao; Sumino, Tatsuo; Tsuneda, Satoshi

doi:10.1007/s00253-008-1739-0

Cited by 84 publications

(29 citation statements)

References 17 publications

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“…Kuenenia stuttgartiensis’ and ‘ Ca . Jettenia caeni’ cells, in which addition of 5 mM methanol decreased anammox activities by only 29% (Isaka et al ., ). These observations suggest that methanol is not a universal inhibitor of anammox bacteria.…”

Section: Discussionmentioning

confidence: 97%

Physiological characterization of anaerobic ammonium oxidizing bacterium ‘Candidatus Jettenia caeni’

Ali

Oshiki

Awata

et al. 2014

Environmental Microbiology

216

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To date, six candidate genera of anaerobic ammonium-oxidizing (anammox) bacteria have been identified, and numerous studies have been conducted to understand their ecophysiology. In this study, we examined the physiological characteristics of an anammox bacterium in the genus 'Candidatus Jettenia'. Planctomycete KSU-1 was found to be a mesophilic (20-42.5°C) and neutrophilic (pH 6.5-8.5) bacterium with a maximum growth rate of 0.0020 h(-1) . Planctomycete KSU-1 cells showed typical physiological and structural features of anammox bacteria; i.e. (29) N2 gas production by coupling of (15) NH4 (+) and (14) NO2 (-) , accumulation of hydrazine with the consumption of hydroxylamine and the presence of anammoxosome. In addition, the cells were capable of respiratory ammonification with oxidation of acetate. Notably, the cells contained menaquinone-7 as a dominant respiratory quinone. Proteomic analysis was performed to examine underlying core metabolisms, and high expressions of hydrazine synthase, hydrazine dehydrogenase, hydroxylamine dehydrogenase, nitrite/nitrate oxidoreductase and carbon monoxide dehydrogenase/acetyl-CoA synthase were detected. These proteins require iron or copper as a metal cofactor, and both were dominant in planctomycete KSU-1 cells. On the basis of these experimental results, we proposed the name 'Ca. Jettenia caeni' sp. nov. for the bacterial clade of the planctomycete KSU-1.

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Section: Discussionmentioning

confidence: 97%

Physiological characterization of anaerobic ammonium oxidizing bacterium ‘Candidatus Jettenia caeni’

Ali

Oshiki

Awata

et al. 2014

Environmental Microbiology

216

View full text Add to dashboard Cite

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“…Synthetic medium was continuously supplied into the reactor. The composition of the mineral medium and trace element solution fed to the reactor have been described by Isaka . Ammonium and nitrite were added as a mineral medium in the form of NH 4 Cl and NaNO 2 .…”

Section: Methodsmentioning

confidence: 99%

ANAMMOX performance, granulation, and microbial response under COD disturbance

Huang

Ruan

et al. 2014

J. Chem. Technol. Biotechnol.

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BACKGROUND: ANAMMOX (ANaerobic AMMonium OXidation), a promising process for biological nitrogen removal, is usually affected by organic matter. In order to fully understand the effect of chemical oxygen demand (COD) on the ANAMMOX process, reactor performance, granular characteristics and microbial response were evaluated systematically under glucose stimulation. RESULTS: High removal efficiencies of ammonium and total nitrogen (> 90%) were achieved with 100 mg L -1 COD concentration. ANAMMOX contribution was reduced to only 69% as COD concentration was increased to 300 mg L -1 . The granulation, EPS contents, TB-EPS/LB-EPS and protein/carbohydrate were increased at a specific COD, with higher COD disrupting the biofacies structure. The results also showed that EPS distribution and microbial structure were affected by the organic disturbance. CONCLUSION: An appropriate COD concentration enhanced the total nitrogen removal during ANAMMOX process, by enabling a stable synergism between ANAMMOX bacteria and heterotrophic denitrificans. Furthermore, a proper COD stimulation promoted sludge granulation by influencing EPS composition and distribution. However, excessive COD disturbance disrupted the highly efficient biofacies structure and microbial balance, which then decreased the ANAMMOX competitiveness.

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“…Kuenenia stuttgartiensis'' and ''Ca. Jettenia caeni'' cells, where addition of 5 mM methanol decreased only 29% of anammox activities (Isaka et al, 2008b). Nonetheless, practical evidences are required to further nail down ecological niche partitioning of anammox bacteria in various ecosystems and vital to study microbial competition among various anammox bacterial strains during long-term cultivation.…”

Section: Ecophysiology and Niche Partitioning Of Anammox Bacteriamentioning

confidence: 99%