Anaerobic degradation of halogenated benzoic acids by photoheterotrophic bacteria

Woude, Bernard J. van der; Boer, M. de; Put, N.M.J. van der; Geld, F.M. van der; Prins, R. A.; Gottschal, Jan C.

doi:10.1111/j.1574-6968.1994.tb06889.x

Cited by 38 publications

(15 citation statements)

References 25 publications

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“…Several strains of R. palustris with the ability to metabolize halogenated aromatic compounds have been isolated from a range of environments, including soils, aquatic sediments, and sewage sludge (Kamal and Wyndham , van der Woude et al. , Egland et al. ).…”

Section: Discussionmentioning

confidence: 99%

Urban stream microbial communities show resistance to pharmaceutical exposure

et al. 2018

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Abstract. Residues of pharmaceuticals are increasingly detected in surface waters throughout the world.In four streams in Baltimore, Maryland, USA, we detected analgesics, stimulants, antihistamines, and antibiotics using passive organic samplers. We exposed biofilm communities in these streams to the common drugs caffeine, cimetidine, ciprofloxacin, and diphenhydramine. Respiration rates in the least urban stream were suppressed when exposed to these drugs, but biofilm functioning in the most urban stream was resistant to drug exposure. Exposure to the antibiotic ciprofloxacin altered bacterial community composition at all sites, with the greatest change occurring in the most urban stream. These results indicated that continuous exposure to drugs in urban streams may select for sub-populations of highly resistant bacteria that maintain community function in response to urban contaminants.

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Section: Discussionmentioning

confidence: 99%

Urban stream microbial communities show resistance to pharmaceutical exposure

et al. 2018

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Section: Methodsmentioning

confidence: 99%

“…In this study the phototrophic purple non‐sulfur bacterium R. palustris strain DCP3 [31] was used as a model organism. Low‐chloride minimal (LCM) medium [31] was used for both aerobic and anaerobic cultivation. After autoclaving of the basal LCM medium, 2 ml of filter‐sterilized vitamin solution was added per liter [31] and 25 ml of 1 M autoclaved K(NH 4 )PO 4 solution (pH 7.0) was added per liter resulting in a final concentration of 25 mM.…”

Section: Methodsmentioning

confidence: 99%

“…R. palustris is known for its ability to utilize a very wide range of growth substrates [27–29], including halogenated aromatic compounds [30,31]. For example, R. palustris strain DCP3 is capable of growth on chlorinated benzoates [31] and hence may play a role in dechlorination of aromatic compounds in aquatic sediments. However, very little is known about the actual abundance and spatial distribution of this and related strains in such environments.…”

Section: Introductionmentioning

confidence: 99%

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Influence of growth rate and starvation on fluorescent in situ hybridization of Rhodopseudomonas palustris

Oda

Slagman

Meijer

et al. 2000

FEMS Microbiology Ecology

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In situ hybridization with a fluorescently labeled 16S rRNA-targeted probe was examined using Rhodopseudomonas palustris as a model organism, which had been grown at different rates and under different conditions of growth and starvation. The specific growth rate did not affect the percentage of hybridized cells in aerobically grown R. palustris cultures. However, significant changes in the percentage of hybridized cells occurred during extended periods of starvation. These changes were observed both in batch cultures grown and starved aerobically in the dark, and in cultures grown phototrophically and starved anaerobically in the dark. Aerobic growth in batch culture and subsequent starvation resulted in a complete lack of detectable hybridization after 20 days of starvation. In contrast, even after 30 days of starvation, 50% of all cells were still detectable in cultures grown aerobically at growth rates <0.06 h(-1) and then starved aerobically in the dark. The same was true for phototrophically grown cells that were starved anaerobically in the light. During starvation there was a clear, though non-linear, positive correlation between the percentage of hybridized cells and the RNA content. In contrast, no direct correlation was observed between the number of hybridized cells in a culture and the viability of this culture. Thus, in habitats with growing, non-growing, and starving bacteria, data on quantitative detection of populations based on 16S rRNA-targeted probing should be used with extreme caution as the detectability of the individual cells is strongly influenced by their physiological history and current physiological state.

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“…Batch cultures were routinely incubated under saturating light conditions at 30°C in screw‐cap tubes (16 ml) containing anoxically prepared low‐chloride minimal (LCM) medium (with 0.01% of yeast extract) [4] and an N 2 gas phase. After autoclaving of the basal LCM medium, 25 ml of 1 M autoclaved K(NH 4 )PO 4 solution (pH 7.0) was added per liter to a final concentration of 25 mM and 2 ml of filter‐sterilized vitamin solution [4] was added per liter to the medium. Carbon sources were added from separately autoclaved stock solutions at the concentrations indicated in the text.…”

Section: Methodsmentioning

confidence: 99%

Acquisition of the ability for Rhodopseudomonas palustris to degrade chlorinated benzoic acids as the sole carbon source

Oda

Vries

Forney

et al. 2001

FEMS Microbiology Ecology

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Three strains of Rhodopseudomonas palustris were isolated from phototrophic enrichment cultures containing 3‐chlorobenzoate (3‐CBA) and benzoate (BA). These new strains as well as several previously described strains of R. palustris were tested in this study and shown to degrade 3‐CBA if grown in media that contained BA as a co‐substrate. All of the pure cultures that originally required BA for the degradation of 3‐CBA acquired the ability to degrade 3‐CBA as the sole carbon source after long periods of incubation that ranged from 1 to 3 months. After this adaptation period, the 3‐CBA‐degrading capabilities of all variants were stable, and the rates of 3‐CBA degradation were significantly enhanced as compared to the parental strains. Furthermore, the variants had also acquired the ability to metabolize 2‐ and 4‐CBA as sole carbon sources indicating that the enhanced ability to metabolize 3‐CBA was accompanied by an expanded ability to metabolize chlorinated benzoates. These data indicate that acquisition of the ability to degrade 3‐CBA may be rather common among strains of R. palustris and mutations that confer the ability to metabolize 3‐CBA may provide a selective advantage to R. palustris under specific environmental conditions.

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Anaerobic degradation of halogenated benzoic acids by photoheterotrophic bacteria

Cited by 38 publications

References 25 publications

Urban stream microbial communities show resistance to pharmaceutical exposure

Urban stream microbial communities show resistance to pharmaceutical exposure

Influence of growth rate and starvation on fluorescent in situ hybridization of Rhodopseudomonas palustris

Acquisition of the ability for Rhodopseudomonas palustris to degrade chlorinated benzoic acids as the sole carbon source

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