Analgesic properties of extracts and fractions from Erythrina crista-galli (Fabaceae) leaves

Abstract

The present study describes the analgesic activity of extracts and some fractions obtained from Erythrina crista-galli leaves in different in vivo analgesic models, using mice as experimental animals. The results showed that extract E(2) was the most active, inhibiting 48% of the abdominal constrictions when evaluated against the writhing test at 10 mg kg(-1), intraperitoneal. It also caused dose-dependent inhibition in the same model, with a calculated ID(50) value and respective confidence interval of 10 (9-14) mg kg(-1), and was more potent than reference drugs. Administered orally, E(2) caused potent antinociceptive action, with a calculated ID(50) value of 35 (26-47) mg kg(-1). The fractions F(1) and F(2) obtained from E(2) were evaluated against the writhing test at 10 mg kg(-1), causing inhibitions of 41 and 88%, respectively. The most active fraction, F(2), presented ID(50) calculated value of 3 (2-4) mg kg(-1), being about 7-fold more active than the reference drugs (acetyl salicylic acid and acetaminophen). In the formalin test, F(2) inhibited both phases of pain (44%, first phase; 58%, second phase). However, in contrast to the results observed for E(2), it was not active against the hot-plate test. The phytochemical results showed that at least four main components are present in F(2), which show a positive reaction of terpenes with TLC spray reagents.

LC-MS/MS and GC/MS Profiling of Petroselinum sativum Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats

“…Other studies showed the analgesic effect of the ethanolic extract of parsley prepared at 100, 150, and 200 mg/kg acts by decreasing both phases of pain using the formalin test [ 19 ]. Using this assay, there is two distinct biphasic nociceptive responses known as early and late phases [ 66 ]. Molecules that target precisely the central nervous system (CNS) can inhibit both phases by a similar mechanism.…”

Antinociceptive Activity of the Chloroform Fraction of Dioclea virgata (Rich.) Amshoff (Fabaceae) in Mice

“…Among other plant species of the family Fabaceae evaluated in the acetic acid methodology, it may be mentioned that the extract of Erythrina velutina and Erythrina mulungu , the aqueous extract of Desmodium gangeticum DC, as well as the extract and some fractions from Erythrina crista-galli , reduced the number of writhings in rodent studies [8, 45, 46]. …”

Erythrina, a Potential Source of Chemicals from the Neotropics

“…The analgesic properties of extracts and fractions from E. crista-galli leaves were tested by Fischer, et al, (2007) and they found that one of the fractions was the most active, being about 7-fold more active than the reference drugs(acetyl salicylic acid and acetaminophen)…”