Analyse von Globulinen mit der Gelfiltration

Wirth, K; Ullmann, U.; Brand, K.; Huth, K; Hess, Bettina

doi:10.1007/bf01485114

Cited by 17 publications

(2 citation statements)

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“…Gel filtration was performed on Sephadex G-200 (Pharmacia, Uppsala). Column size was 45 x 2.5 cm using the method of Wirth et al [25]. 2 ml of serum were applied to the column for each chromatography estimation.…”

Section: Clinical Studiesmentioning

confidence: 99%

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

1972

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γ-GT serum levels have been compared in various liver diseases with AP and LAP, cholestatic markers ; with GOT and GPT, the increases of which are related to cellular injury. γ-GT levels have also been assayed in serum and in hepatic tissue of rats after poisoning with CCl₄ and after bile duct ligation. The results showed in all cases a significant correlation between γ-GT and AP, and γ-GT and LAP. A significant increase of γ-GT was found in plasma and in hepatic tissue of rats after bile duct ligation; on the other hand normal levels were found in the plasma of rats after treatment with CCl₄. γ-GT gelfiltration on Sephadex G-200 showed one peak only of activity in all cases considered, eluted with the first protein fraction. The enzymes seem to be rather homogeneous with a molecular weight of about 200,000.

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Section: Clinical Studiesmentioning

confidence: 99%

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

1972

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“…Serum fractionation on Sephadex G 200 (Pharmacia, Uppsala, Sweden) columms was performed according to Wirth et al [28]. Aliquots of 2 ml were collected in separate tubes.…”

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confidence: 99%

Heterogeneity of Serum γ-Glutamyl Transpeptidase in Different Internal Diseases Studied by Starch Gel Electrophoresis and Sephadex Filtration

Kokot¹,

J²

1968

Enzymol Biol Clin

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Summary: γ-glutamyl transpeptidase of human serum studied by starch gel electrophoresis and Sephadex filtration is not homogenous. Excepting liver cirrhosis no distinct distribution pattern of activity specific to any hepatobiliary disease or other internal diseases were found. Studies of GGTP heterogeneity seems to be of no great clinical significance in the differential diagnosis of hepatic or extrahepatic diseases.

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Materials and Methods

Determann¹

1969

Gel Chromatography

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Analyse von Globulinen mit der Gelfiltration

Cited by 17 publications

References 3 publications

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

Heterogeneity of Serum γ-Glutamyl Transpeptidase in Different Internal Diseases Studied by Starch Gel Electrophoresis and Sephadex Filtration

Materials and Methods

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