Analysing the effect of dietary prebiotics and probiotics on gut bacterial richness and diversity of Asian snakehead fingerlings using T-RFLP method

Abstract

This study was designed to evaluate the effects of three prebiotics (β-glucan, galacto-oligosaccharide [GOS], mannan-oligosaccharide [MOS]) and two probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus) on the microbiome of snakehead during growth of fingerlings. In addition, the experiment evaluated the capacity of Channa striata fingerlings to retain the benefits derived from these supplements after withdrawal. Throughout the study, it was observed that supplementation with dietary prebiotics and probiotics led to significant (p < 0.05) change in gut bacterial profile and improvement in gut morphology. Terminal restriction fragment length polymorphism (T-RFLP) was used for the comparative analysis of gut communities and all 46 of the T-RFLP detected phylotypes were present in the Lactobacillus supplemented fish, while significantly fewer were detected in controls and other experimental supplement regimes. Histological studies and electron microscopy revealed that both the prebiotic and probiotic treated fish had significantly longer and wider villi and deeper crypts compared to the controls. The microvilli length, as evaluated with electron microscopy, was also longer in all treated fish compared to controls.Furthermore, this study is the first to report the absence of differences in sustaining the efficacies attained after intake of β-glucan, GOS, MOS and live yeast upon postfeeding with an unsupplemented feed, over a prolonged period.

Autochthonous bacterium Lactobacillus plantarum as probiotic supplementation for productive performance and sanitary improvements on clownfish Amphiprion ocellaris

“…In the present study, intestinal modulation through bacterial colonization and changes in intestinal morphology explain the superior performance. The presence of probiotic bacteria in the intestinal tract during an unbroken feed period promotes the growth of villi and consequently greater nutrient absorption (Sayes et al, 2017;Munir et al, 2018). Thus, higher weight gain or better specific growth rate represent some of the benefits of autochthonous bacteria that have been reported in the literature and observed in clownfish.…”

Multi‐species probiotics enhance growth of Nile tilapia ( Oreochromis niloticus ) through upgrading gut, liver and muscle health

“…It has been reported that probiotics boosted growth, enzymatic contribution to nutrition, suppression of adhesion and colonization of harmful bacteria in the digestive tract, regulation of the gut microbiota and increased haematological parameters and immunological response of shellfish (Ringø, 2020). Dietary probiotics ( Saccharomyces cerevisiae , Lactobacillus acidophilus ) supplementation resulted in a significant improvement in the gut microbial profile and improvement in gut morphology of Asian snakehead ( Channa striata ) fingerlings (Munir et al, 2018). Probiotics added to water during the early stages of growth had the most beneficial effects in the survival of yellow perch, Perca flavescens (Shaheen et al, 2014).…”

Assessing the influence of the inclusion of Bacillus subtilis AQUA‐GROW ® as feed additive on the growth performance, feed utilization, immunological responses and body composition of the Pacific white shrimp, Litopenaeus vannamei

“…Dietary probiotics are considered as a bioremediation and environmental friendly tool for the aquaculture development and sustainability (Ahmad et al, 2017; Eissa et al, 2022; Zibiene & Zibas, 2019), as they have proven to be a great substitute for antibiotics developing the beneficial microflora in the digestive truck of the fish (Huerta‐Rábago et al, 2019; Kavitha et al, 2018; Mamun et al, 2019; Munir, Terence, et al, 2018; Wang et al, 2020). Therefore, fish fed with dietary probiotic supplement exhibited the enhancement of growth performance and health status of several studied fishes like Malaysian mahseer (Sharifah Lia et al, 2022), Asian snakehead (Munir, Roshada, Abdul‐Manaf, & Azizah, 2016; Munir, Roshada, et al, 2018; Munir, Roshada, Yam, et al, 2016; Munir, Terence, et al, 2018; Talpur et al, 2014), common carp and grass carp (Sahandi et al, 2012), tilapia fish (Mohamed et al, 2013).…”